<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Phytochemical constituents of plants extracts have been well investigated as leads for drug discovery and development. However, aside their immense medicinal properties, their safety is also of paramount importance, therefore evaluating the toxicity of plants extracts and their phytochemicals is an important aspect of quality measures to meet the requirements for consumption.This study was done to identify the volatile phytochemical compounds present in n-hexane extract of <italic>Uvaria chamae</italic> stems (UCSH) with the aid of gas chromatography-mass spectrometry (GC-MS) and to evaluate the effects of this non-polar extract on DNA using diphenylamine (DPA) and agarose gel electrophoresis assays, also to investigate the haematological parameters using an auto Haematology Analyzer.The GC-MS analysis of UCSH identified some compounds which include; squalene, lupeol, stigmasterol, β-sitosterol, and Benzyl benzoate with excellent medicinal properties. Genotoxicity assays results showed significant reductions in DNA fragmentation in liver and testis cells of mice as against the DNA biomarker and vehicle control group. Haematological results showed significant increase in white blood cells (WBC), red blood cell (RBC), platelets (PLT) and lymphocytes (LYMP) but significant reduction in mean corpuscular - volume (MCV), haemoglobin (MCH), and haemoglobin concentration (MCHC).The present study showed that the phytochemicals present in the n-Hexane extract of <italic>Uvaria chamae</italic> stems may play protective role in the genomic DNA of liver and testis tissues and also may induce hematological changes which may enhance the immune system.</p></abstract>

Phytochemical constituents of plants extracts have been well investigated as leads for drug discovery and development. However, aside their immense medicinal properties, their safety is also of paramount importance, therefore evaluating the toxicity of plants extracts and their phytochemicals is an important aspect of quality measures to meet the requirements for consumption.This study was done to identify the volatile phytochemical compounds present in n-hexane extract of Uvaria chamae stems (UCSH) with the aid of gas chromatography-mass spectrometry (GC-MS) and to evaluate the effects of this non-polar extract on DNA using diphenylamine (DPA) and agarose gel electrophoresis assays, also to investigate the haematological parameters using an auto Haematology Analyzer.The GC-MS analysis of UCSH identified some compounds which include; squalene, lupeol, stigmasterol, β-sitosterol, and Benzyl benzoate with excellent medicinal properties. Genotoxicity assays results showed significant reductions in DNA fragmentation in liver and testis cells of mice as against the DNA biomarker and vehicle control group. Haematological results showed significant increase in white blood cells (WBC), red blood cell (RBC), platelets (PLT) and lymphocytes (LYMP) but significant reduction in mean corpuscular - volume (MCV), haemoglobin (MCH), and haemoglobin concentration (MCHC).The present study showed that the phytochemicals present in the n-Hexane extract of Uvaria chamae stems may play protective role in the genomic DNA of liver and testis tissues and also may induce hematological changes which may enhance the immune system.

Haematological and Genotoxicity Evaluations of Phytochemical Compounds from n-Hexane Extract of <italic>Uvaria chamae</italic> Stem on Selected Organs in Mice

Department of Pharmaceutical Chemistry, Faculty of Pharmacy

Annals of Science and Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Haematological and Genotoxicity Evaluations of Phytochemical Compounds from n-Hexane Extract of  Stem on Selected Organs in Mice

Phytochemical constituents of plants extracts have been well investigated as leads for drug discovery and development. However, aside their immense medicinal...

Haematological and Genotoxicity Evaluations of Phytochemical Compounds from n-Hexane Extract of Uvaria chamae Stem on Selected Organs in Mice

Published Online: Aug 29, 2018

Page range: 28 - 34

Received: Apr 13, 2018

Accepted: Jun 06, 2018

DOI: https://doi.org/10.2478/ast-2018-0012

Keywords
Liver, Testis, Genotoxicity, Haematology

© 2018 Oladipupo R. Akolade et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Haematological and Genotoxicity Evaluations of Phytochemical Compounds from n-Hexane Extract of Uvaria chamae Stem on Selected Organs in Mice

Published Online: Aug 29, 2018

Page range: 28 - 34

Received: Apr 13, 2018

Accepted: Jun 06, 2018

DOI: https://doi.org/10.2478/ast-2018-0012

KeywordsLiver, Testis, Genotoxicity, Haematology

© 2018 Oladipupo R. Akolade et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Liver, Testis, Genotoxicity, Haematology