<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to evaluate antioxidant, antibacterial, and anticholinesterase activities of different extracts of an uninvestigated endemic species Bellevalia mauritanica Pomel, and identification of its phenolic and flavonoid content by a comprehensive and validated LC-MS/MS method using 37 phytochemical standards. Moreover, the phytochemical profiling of the studied extracts was accomplished by LC-MS-IT-TOF technique. Among all the tested extracts, ethyl acetate (EtOAc) of BRCE (bulbs-roots crude extract) was the most active one. It exhibited a medium antibacterial activity, good inhibition of acetylcholinesterase and a good antioxidant activity in ABTS•+ (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonique), DPPH• (1,1’-diphenyl-2-picrylhydrazyl radical), and superoxyde DMSO (dimethyl sulfoxide) alkaline scavenging assays. LC-MS/MS analysis revealed that EtOAc extract had caffeic acid (1240.7 µg/g extract) and chloroform extract had vanillin (2048.5 µg/g extract) in considerable amounts. They also contained good amounts of certain phytochemicals such as protocatechuic acid (274.3 and 145.2 µg/g extract), p-coumaric acid (382.8 and 310.9 µg/g extract), ferulic acid (361.2 and 969.4 µg/g extract) and kaempferol (146.7 and 150.6 µg/g extract) respectively. LC-MS-IT-TOF screening revealed the presence of 91 major phytochemicals in the three extracts derived from bulb-roots crude extract.</abstract>

The aim of this study was to evaluate antioxidant, antibacterial, and anticholinesterase activities of different extracts of an uninvestigated endemic species Bellevalia mauritanica Pomel, and identification of its phenolic and flavonoid content by a comprehensive and validated LC-MS/MS method using 37 phytochemical standards. Moreover, the phytochemical profiling of the studied extracts was accomplished by LC-MS-IT-TOF technique. Among all the tested extracts, ethyl acetate (EtOAc) of BRCE (bulbs-roots crude extract) was the most active one. It exhibited a medium antibacterial activity, good inhibition of acetylcholinesterase and a good antioxidant activity in ABTS•+ (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonique), DPPH• (1,1’-diphenyl-2-picrylhydrazyl radical), and superoxyde DMSO (dimethyl sulfoxide) alkaline scavenging assays. LC-MS/MS analysis revealed that EtOAc extract had caffeic acid (1240.7 µg/g extract) and chloroform extract had vanillin (2048.5 µg/g extract) in considerable amounts. They also contained good amounts of certain phytochemicals such as protocatechuic acid (274.3 and 145.2 µg/g extract), p-coumaric acid (382.8 and 310.9 µg/g extract), ferulic acid (361.2 and 969.4 µg/g extract) and kaempferol (146.7 and 150.6 µg/g extract) respectively. LC-MS-IT-TOF screening revealed the presence of 91 major phytochemicals in the three extracts derived from bulb-roots crude extract.

Chemical profiling and <italic>in vitro</italic> antioxidant, antibacterial and anticholinesterase activities of an endemic North African species <italic>Bellevalia mauritanica</italic> Pomel

Laboratoire de Génétique, Biochimie et Biotechnologies Végétales GBBV, faculté des Sciences de la nature et de la vie

Université Frères Mentouri Constantine1

Centre National de Recherches en biotechnologies CRBt. Ali Mendjli Nouvelle Ville

Dicle University Science and Technology Research and Application Center, Dicle University

Université Frères Mentouri, Constantine 1

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chemical profiling and  antioxidant, antibacterial and anticholinesterase activities of an endemic North African species  Pomel

The aim of this study was to evaluate antioxidant, antibacterial, and anticholinesterase activities of different extracts of an uninvestigated endemic species...

Chemical profiling and in vitro antioxidant, antibacterial and anticholinesterase activities of an endemic North African species Bellevalia mauritanica Pomel

Published Online: Dec 08, 2020

Page range: 26 - 45

DOI: https://doi.org/10.2478/asn-2020-0031

Keywords
Pomel, antioxidant, acetylcholinesterase, antibacterial, LC-MS/MS, LC-MS-IT-TOF

© 2020 Rayene Ouelbani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chemical profiling and in vitro antioxidant, antibacterial and anticholinesterase activities of an endemic North African species Bellevalia mauritanica Pomel

Published Online: Dec 08, 2020

Page range: 26 - 45

DOI: https://doi.org/10.2478/asn-2020-0031

KeywordsPomel, antioxidant, acetylcholinesterase, antibacterial, LC-MS/MS, LC-MS-IT-TOF

© 2020 Rayene Ouelbani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Pomel, antioxidant, acetylcholinesterase, antibacterial, LC-MS/MS, LC-MS-IT-TOF