<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Fenugreek is an herb that has the ability to resist multiple environmental insults, pests, diseases and many pathogens through the synthesis of substances allowing this plant protection, these substances are called phytochemical compounds or secondary metabolites. The aim of this study is to characterize these secondary metabolites in the extracts of fenugreek seeds grown in Algeria and compare the phytochemical profile and antioxidant activity of the aqueous extracts obtained by maceration and decoction. We carried out a “phytochemical screening” on both types of extracts in order to highlight the different classes of secondary metabolites and assaying total polyphenols by the method of Follin ciocaltchu, total alkaloids and tannins contained in these aqueous extracts and appreciate their antioxidant activity using the FRAP technique. A characterization of phytochemical molecules of the two types of Fenugreek extracts was done by chromatographic method with HPLC. Several phytochemical groups were identified in the extracts of fenugreek: tannins, saponins, flavonoids, terpenes and reducing nitrogen compounds. Chromatographic analysis of the samples show that 4 phytochemical molecules were identified in the aqueous maceration extract: kaempferol, genistein, vanillin and myrecitine and 3 molecules for the aqueous extract of decoction are: rutin, kaempferol and vanillin. The aqueous extract of decoction was characterized by the total polyphenols content (31.7 ± 0.031 mg GAE / 100 g of extract), total alkaloids (2.12 ± 0.015%) and tannins (9.75 ± 0.06%) that are higher than those obtained in the aqueous extract of maceration: total polyphenols (18.9 ± 0.12 mg GAE / 100g of extract), total alkaloids (1.71 ± 0.02%) and 8.69 ± 0.02% for the tannins. A non-significant difference was marked for antioxidant activity found in both types of extracts (56.90 ± 0.013 mg AAE / 100g decoction extract against 56.37 ± 0.24 mg AAE / 100g maceration extract). In the end we conclude that fenugreek may be a promising plant genetic resource responsible for an important biological activity, except its nutritional and therapeutic properties due to their phytochemical compounds.</p></abstract>

Fenugreek is an herb that has the ability to resist multiple environmental insults, pests, diseases and many pathogens through the synthesis of substances allowing this plant protection, these substances are called phytochemical compounds or secondary metabolites. The aim of this study is to characterize these secondary metabolites in the extracts of fenugreek seeds grown in Algeria and compare the phytochemical profile and antioxidant activity of the aqueous extracts obtained by maceration and decoction. We carried out a “phytochemical screening” on both types of extracts in order to highlight the different classes of secondary metabolites and assaying total polyphenols by the method of Follin ciocaltchu, total alkaloids and tannins contained in these aqueous extracts and appreciate their antioxidant activity using the FRAP technique. A characterization of phytochemical molecules of the two types of Fenugreek extracts was done by chromatographic method with HPLC. Several phytochemical groups were identified in the extracts of fenugreek: tannins, saponins, flavonoids, terpenes and reducing nitrogen compounds. Chromatographic analysis of the samples show that 4 phytochemical molecules were identified in the aqueous maceration extract: kaempferol, genistein, vanillin and myrecitine and 3 molecules for the aqueous extract of decoction are: rutin, kaempferol and vanillin. The aqueous extract of decoction was characterized by the total polyphenols content (31.7 ± 0.031 mg GAE / 100 g of extract), total alkaloids (2.12 ± 0.015%) and tannins (9.75 ± 0.06%) that are higher than those obtained in the aqueous extract of maceration: total polyphenols (18.9 ± 0.12 mg GAE / 100g of extract), total alkaloids (1.71 ± 0.02%) and 8.69 ± 0.02% for the tannins. A non-significant difference was marked for antioxidant activity found in both types of extracts (56.90 ± 0.013 mg AAE / 100g decoction extract against 56.37 ± 0.24 mg AAE / 100g maceration extract). In the end we conclude that fenugreek may be a promising plant genetic resource responsible for an important biological activity, except its nutritional and therapeutic properties due to their phytochemical compounds.

Phytochemistry, HPLC profile and antioxidant activity of aqueous extracts of fenugreek (<italic>Trigonella foenum graecum</italic> L.) seeds grown in arid zones of Algeria

Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Zones

Laboratory of Plant Physiology Applied to Soilless Crops

Laboratory of Hygiene and Animal Pathology

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phytochemistry, HPLC profile and antioxidant activity of aqueous extracts of fenugreek ( L.) seeds grown in arid zones of Algeria

Fenugreek is an herb that has the ability to resist multiple environmental insults, pests, diseases and many pathogens through the synthesis of substances...

Phytochemistry, HPLC profile and antioxidant activity of aqueous extracts of fenugreek (Trigonella foenum graecum L.) seeds grown in arid zones of Algeria

Published Online: Nov 08, 2019

Page range: 71 - 87

DOI: https://doi.org/10.2478/asn-2019-0020

Keywords
L., aqueous extract, phytochemistry, antioxidant activity, HPLC, Algeria

© 2019 Mohammed Nasir Assam Benziane et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phytochemistry, HPLC profile and antioxidant activity of aqueous extracts of fenugreek (Trigonella foenum graecum L.) seeds grown in arid zones of Algeria

Published Online: Nov 08, 2019

Page range: 71 - 87

DOI: https://doi.org/10.2478/asn-2019-0020

KeywordsL., aqueous extract, phytochemistry, antioxidant activity, HPLC, Algeria

© 2019 Mohammed Nasir Assam Benziane et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
L., aqueous extract, phytochemistry, antioxidant activity, HPLC, Algeria