<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aerial parts of Tamarix gallica L. taken from three different climatic stages in Algeria (Oum El Bouaghi: Semi-arid, ElTaref: Humid, and Ouargla: Arid) were extracted using boiling distilled water. The crude extracts were subjected to total phenolics and flavonoids quantifications in addition to anti-proliferative assessment against two tumor cell lines namely rat brain tumor (C6) and human cervix carcinoma (HeLa) using BrdU (bromo-deoxyuridine) ELISA(Enzyme-linked immune-sorbent assay) and xCELLigence assay. The total phenolics yield was found to range between 16.14 and 39.32 mg GAE (Gallic acid equivalent)/g of extract and a flavonoids yield ranging between 16.51 and 20.35 mg QE(quercetin equivalent)/g of extract. The various phenolics were identified using HPLC-TOF/MS to highlight hesperidin and rosmarinic Acid as major components. Moreover, the extracts exhibited different levels of antitumor potency against C6 and HeLa cell lines depending upon the climatic stage and the concentration. A good cytotoxic effect was recorded with the species collected from the humid region at 250 μg/mL. On the contrary, the other extracts revealed a weak activity for both tests.</p></abstract>

The aerial parts of Tamarix gallica L. taken from three different climatic stages in Algeria (Oum El Bouaghi: Semi-arid, ElTaref: Humid, and Ouargla: Arid) were extracted using boiling distilled water. The crude extracts were subjected to total phenolics and flavonoids quantifications in addition to anti-proliferative assessment against two tumor cell lines namely rat brain tumor (C6) and human cervix carcinoma (HeLa) using BrdU (bromo-deoxyuridine) ELISA(Enzyme-linked immune-sorbent assay) and xCELLigence assay. The total phenolics yield was found to range between 16.14 and 39.32 mg GAE (Gallic acid equivalent)/g of extract and a flavonoids yield ranging between 16.51 and 20.35 mg QE(quercetin equivalent)/g of extract. The various phenolics were identified using HPLC-TOF/MS to highlight hesperidin and rosmarinic Acid as major components. Moreover, the extracts exhibited different levels of antitumor potency against C6 and HeLa cell lines depending upon the climatic stage and the concentration. A good cytotoxic effect was recorded with the species collected from the humid region at 250 μg/mL. On the contrary, the other extracts revealed a weak activity for both tests.

Water Extract of <italic>Tamarix gallica</italic> L. as effective Agents against C6 and HeLa tumor cell lines

Faculty of Sciences, Department of Natural and Life sciences

Laboratory of Natural Resources and Management of Sensitive Environments

Laboratory of Organic Synthesis, Modeling and Optimization of Chemical Processes

Department of Chemistry, Faculty of Science

Laboratory of Biomolecules and Plant Breeding

Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature

Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry

Department of food technology, Nutrition and food science, Faculty of veterinary sciences

Acta Scientifica Naturalis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Water Extract of  L. as effective Agents against C6 and HeLa tumor cell lines

The aerial parts of Tamarix gallica L. taken from three different climatic stages in Algeria (Oum El Bouaghi: Semi-arid, ElTaref: Humid, and Ouargla: Arid)...

Water Extract of Tamarix gallica L. as effective Agents against C6 and HeLa tumor cell lines

Published Online: Nov 08, 2019

Page range: 1 - 13

DOI: https://doi.org/10.2478/asn-2019-0015

Keywords
Tamarixgallica, phenolics, flavonoids, antiproliferative activity, C6, HeLa

© 2019 Ouroud Fellah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Water Extract of Tamarix gallica L. as effective Agents against C6 and HeLa tumor cell lines

Published Online: Nov 08, 2019

Page range: 1 - 13

DOI: https://doi.org/10.2478/asn-2019-0015

KeywordsTamarixgallica, phenolics, flavonoids, antiproliferative activity, C6, HeLa

© 2019 Ouroud Fellah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Tamarixgallica, phenolics, flavonoids, antiproliferative activity, C6, HeLa