<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Campylobacter</italic> is highly diverse genetically and also undergoes frequent intraspecific recombination. A major source of campylobacteriosis, which is transmitted to humans is found in poultry. The assessment of the genetic diversity among <italic>Campylobacter</italic> population is critical to our understanding of the epidemiology. The genetic diversity of <italic>Campylobacter jejuni</italic> isolates in broilers and their environment were investigated by <italic>fla</italic>A-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). The study revealed that 92.3% of the examined broiler flocks were contaminated with <italic>Campylobacter</italic> spp. A total number of 35 different <italic>fla</italic>A types defined by <italic>fla</italic>A-RFLP were found in 448 <italic>C. jejuni</italic> isolates originated from broilers, litter, puddles, zones, anteroom and wild birds. The most dominant <italic>fla</italic>A type was XXV. MLST defined 20 sequence types (STs) belonging to 10 clonal complexes (CCs). Among all the STs 9 isolates (15%) were consigned to 2 different STs (ST-7413 and ST-4800), which could not be assigned. The most common CCs were ST-21 and ST-179. The ST-21 CC was common in broilers and environment (puddle water and concentric zones) and the ST-179 CC was specific to wild birds, but also was found in puddle water and concentric zones.</p></abstract>

Campylobacter is highly diverse genetically and also undergoes frequent intraspecific recombination. A major source of campylobacteriosis, which is transmitted to humans is found in poultry. The assessment of the genetic diversity among Campylobacter population is critical to our understanding of the epidemiology. The genetic diversity of Campylobacter jejuni isolates in broilers and their environment were investigated by flaA-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). The study revealed that 92.3% of the examined broiler flocks were contaminated with Campylobacter spp. A total number of 35 different flaA types defined by flaA-RFLP were found in 448 C. jejuni isolates originated from broilers, litter, puddles, zones, anteroom and wild birds. The most dominant flaA type was XXV. MLST defined 20 sequence types (STs) belonging to 10 clonal complexes (CCs). Among all the STs 9 isolates (15%) were consigned to 2 different STs (ST-7413 and ST-4800), which could not be assigned. The most common CCs were ST-21 and ST-179. The ST-21 CC was common in broilers and environment (puddle water and concentric zones) and the ST-179 CC was specific to wild birds, but also was found in puddle water and concentric zones.

Prevalence and Genetic Diversity of <italic>C. Jejuni</italic> Isolated from Broilers and their Environment Using <italic>fla</italic>A-RFLP Typing and MLST Analysis

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence and Genetic Diversity of  Isolated from Broilers and their Environment Using A-RFLP Typing and MLST Analysis

Campylobacter is highly diverse genetically and also undergoes frequent intraspecific recombination. A major source of campylobacteriosis, which is transmitted...

Prevalence and Genetic Diversity of C. Jejuni Isolated from Broilers and their Environment Using flaA-RFLP Typing and MLST Analysis

Published Online: May 04, 2020

Page range: 485 - 501

Received: Nov 06, 2018

Accepted: Jan 03, 2020

DOI: https://doi.org/10.2478/aoas-2020-0008

Keywords
poultry, MLST, RFLP, campylobacteriosis

© 2020 Viktorija Lėgaudaitė Lydekaitienė et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence and Genetic Diversity of C. Jejuni Isolated from Broilers and their Environment Using flaA-RFLP Typing and MLST Analysis

Published Online: May 04, 2020

Page range: 485 - 501

Received: Nov 06, 2018

Accepted: Jan 03, 2020

DOI: https://doi.org/10.2478/aoas-2020-0008

Keywordspoultry, MLST, RFLP, campylobacteriosis

© 2020 Viktorija Lėgaudaitė Lydekaitienė et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
poultry, MLST, RFLP, campylobacteriosis