<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The objective of this study was to determine the relationship between milk β-hydroxybutyrate (BHB), acetone (ACE) as well as parity and lactation stage and milk freezing point (MFP) in Polish Holstein-Friesian cows in early lactation. Additionally, we studied the relationship between milk ketone bodies and daily milk yield (DMY), fat (MF) and protein (MP) content in milk. The data obtained from the Polish Federation of Cattle Breeders and Dairy Farmers, comprised 749,894 test day milk samples, collected between 6 and 60 days in milk (DIM) from 521,049 lactations of 514,066 cows. Milk BHB and ACE were determined using the Fourier transform infrared (FTIR) technology. Four classes of parities were created: first, second, third, and fourth to seventh and two classes of lactation stage: 5–21 and 22–60 DIM. BHB was grouped into five classes: ≤0.05, 0.06–0.10, 0.11–0.20, 0.21–0.50 and >0.50 mmol/L, and ACE was also classified into five classes: ≤0.05, 0.06–0.10, 0.11–0.15, 0.16–0.30 and >0.30 mmol/L. Data on MFP, DMY, and MF and MP content were analyzed using the MIXED procedure of SAS and a linear model in which effects of parity, lactation stage, BHB and ACE classes were included, together with interactions between lactation stage and BHB classes, parity and BHB classes, lactation stage and ACE classes, and parity and ACE classes. The differences among parity, lactation stages, BHB and ACE classes in MFP, DMY, MF and MP were highly significant. There was a clear tendency for decreasing of MFP with increasing of BHB. Such a trend did not occur in case of ACE. DMY and MP decreased and MF increased with increasing BHB or ACE. In conclusion, since MFP can be measured relatively easily and is well related to milk BHB content, it may be used in the prediction of diagnostic models of ketosis based on milk composition.</p></abstract>

The objective of this study was to determine the relationship between milk β-hydroxybutyrate (BHB), acetone (ACE) as well as parity and lactation stage and milk freezing point (MFP) in Polish Holstein-Friesian cows in early lactation. Additionally, we studied the relationship between milk ketone bodies and daily milk yield (DMY), fat (MF) and protein (MP) content in milk. The data obtained from the Polish Federation of Cattle Breeders and Dairy Farmers, comprised 749,894 test day milk samples, collected between 6 and 60 days in milk (DIM) from 521,049 lactations of 514,066 cows. Milk BHB and ACE were determined using the Fourier transform infrared (FTIR) technology. Four classes of parities were created: first, second, third, and fourth to seventh and two classes of lactation stage: 5–21 and 22–60 DIM. BHB was grouped into five classes: ≤0.05, 0.06–0.10, 0.11–0.20, 0.21–0.50 and >0.50 mmol/L, and ACE was also classified into five classes: ≤0.05, 0.06–0.10, 0.11–0.15, 0.16–0.30 and >0.30 mmol/L. Data on MFP, DMY, and MF and MP content were analyzed using the MIXED procedure of SAS and a linear model in which effects of parity, lactation stage, BHB and ACE classes were included, together with interactions between lactation stage and BHB classes, parity and BHB classes, lactation stage and ACE classes, and parity and ACE classes. The differences among parity, lactation stages, BHB and ACE classes in MFP, DMY, MF and MP were highly significant. There was a clear tendency for decreasing of MFP with increasing of BHB. Such a trend did not occur in case of ACE. DMY and MP decreased and MF increased with increasing BHB or ACE. In conclusion, since MFP can be measured relatively easily and is well related to milk BHB content, it may be used in the prediction of diagnostic models of ketosis based on milk composition.

Relationship Between Content of Ketone Bodies in Milk and Milk Freezing Point of Polish Holstein-Friesian Cows in Early Lactation

Department of Genetics, Animal Breeding and Ethology

Department of Animal Nutrition and Biotechnology, and Fisheries

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The objective of this study was to determine the relationship between milk β-hydroxybutyrate (BHB), acetone (ACE) as well as parity and lactation stage and...

Relationship Between Content of Ketone Bodies in Milk and Milk Freezing Point of Polish Holstein-Friesian Cows in Early Lactation

Published Online: May 04, 2020

Page range: 693 - 707

Received: Sep 04, 2019

Accepted: Dec 12, 2019

DOI: https://doi.org/10.2478/aoas-2020-0003

Keywords
acetone, β-hydroxybutyrate, dairy cow, ketosis

© 2020 Agnieszka Otwinowska-Mindur et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Relationship Between Content of Ketone Bodies in Milk and Milk Freezing Point of Polish Holstein-Friesian Cows in Early Lactation

Published Online: May 04, 2020

Page range: 693 - 707

Received: Sep 04, 2019

Accepted: Dec 12, 2019

DOI: https://doi.org/10.2478/aoas-2020-0003

Keywordsacetone, β-hydroxybutyrate, dairy cow, ketosis

© 2020 Agnieszka Otwinowska-Mindur et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
acetone, β-hydroxybutyrate, dairy cow, ketosis