<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to evaluate the effects of different levels of dietary <italic>Lactobacillus plantarum</italic> as probiotic (Pro) with bovine lactoferrin (LF) and xylooligosacharide (XOS) on growth performance, hemato-immune response, body composition, digestive enzymes activity and expression of immune-related and growth-related genes of sobaity (<italic>Sparidentex hasta</italic>) for 8 weeks. Fish were fed with feed including: control diet (no LF, XOS and pro inclusion), diet 1 (400 mg kg−1 LF + 5000 mg kg−1 XOS), diet 2 (400 mg kg−1 LF + 10000 mg kg −1 XOS), diet 3 (400 mg kg−1 LF + 1 × 106 gr−1 Pro (<italic>L. plantarum</italic>)), diet 4 (800 mg kg−1 LF + 5000 mg kg −1 XOS), diet 5 (800 mg kg−1 LF + 10000 mg kg−1 XOS), diet 6 (800 mg kg−1 LF + 1 × 106 gr−1 Pro (<italic>L. plantarum</italic>)). Growth performance, hematological parameters (except for white blood cell counts), body composition and immune-related gene expression were not affected by different experimental groups (p>0.05). Nonetheless, non-specific immune response (except for total immunoglobulin) and growth-related gene expression of treatments and control group significantly varied (P&lt;0.05). Digestive enzymes activity including total protease and amylase increased by supplementing diets with different combinations of immunostimulants (P&lt;0.05). Our results suggest that diets supplemented with selected levels of LF, XOS and <italic>L. plantarum</italic> could not improve growth performance, body composition and hemato-immune response, but improved digestive enzyme activities in <italic>S. hasta</italic> fingerlings.</abstract>

The aim of this study was to evaluate the effects of different levels of dietary Lactobacillus plantarum as probiotic (Pro) with bovine lactoferrin (LF) and xylooligosacharide (XOS) on growth performance, hemato-immune response, body composition, digestive enzymes activity and expression of immune-related and growth-related genes of sobaity (Sparidentex hasta) for 8 weeks. Fish were fed with feed including: control diet (no LF, XOS and pro inclusion), diet 1 (400 mg kg−1 LF + 5000 mg kg−1 XOS), diet 2 (400 mg kg−1 LF + 10000 mg kg −1 XOS), diet 3 (400 mg kg−1 LF + 1 × 106 gr−1 Pro (L. plantarum)), diet 4 (800 mg kg−1 LF + 5000 mg kg −1 XOS), diet 5 (800 mg kg−1 LF + 10000 mg kg−1 XOS), diet 6 (800 mg kg−1 LF + 1 × 106 gr−1 Pro (L. plantarum)). Growth performance, hematological parameters (except for white blood cell counts), body composition and immune-related gene expression were not affected by different experimental groups (p>0.05). Nonetheless, non-specific immune response (except for total immunoglobulin) and growth-related gene expression of treatments and control group significantly varied (P<0.05). Digestive enzymes activity including total protease and amylase increased by supplementing diets with different combinations of immunostimulants (P<0.05). Our results suggest that diets supplemented with selected levels of LF, XOS and L. plantarum could not improve growth performance, body composition and hemato-immune response, but improved digestive enzyme activities in S. hasta fingerlings.

Effects of Single and Combined Supplementation of Dietary Probiotic with Bovine Lactoferrin and Xylooligosaccharide on Hemato-Immunological and Digestive Enzymes of Silvery-Black Porgy (<italic>Sparidentex hasta</italic>) Fingerlings

Department of Aquaculture, Artemia and Urmia Lake Research Institute

Agriculture Research, Education and Extension, South Iran Aquaculture Research Center

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of Single and Combined Supplementation of Dietary Probiotic with Bovine Lactoferrin and Xylooligosaccharide on Hemato-Immunological and Digestive Enzymes of Silvery-Black Porgy () Fingerlings

The aim of this study was to evaluate the effects of different levels of dietary Lactobacillus plantarum as probiotic (Pro) with bovine lactoferrin (LF) and...

Effects of Single and Combined Supplementation of Dietary Probiotic with Bovine Lactoferrin and Xylooligosaccharide on Hemato-Immunological and Digestive Enzymes of Silvery-Black Porgy (Sparidentex hasta) Fingerlings

Published Online: Jan 28, 2020

Page range: 137 - 155

Received: Jun 08, 2019

Accepted: Aug 22, 2019

DOI: https://doi.org/10.2478/aoas-2019-0058

Keywords
digestive enzymes, growth performance, innate immunity, lactoferrin, sobaity ()

© 2020 Vahid Morshedi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of Single and Combined Supplementation of Dietary Probiotic with Bovine Lactoferrin and Xylooligosaccharide on Hemato-Immunological and Digestive Enzymes of Silvery-Black Porgy (Sparidentex hasta) Fingerlings

Published Online: Jan 28, 2020

Page range: 137 - 155

Received: Jun 08, 2019

Accepted: Aug 22, 2019

DOI: https://doi.org/10.2478/aoas-2019-0058

Keywordsdigestive enzymes, growth performance, innate immunity, lactoferrin, sobaity ()

© 2020 Vahid Morshedi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
digestive enzymes, growth performance, innate immunity, lactoferrin, sobaity ()