<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The coding sequences (CDS) of <italic>TFAM</italic> and <italic>TFB2M</italic> genes from Jiaxing Black Pig (JBP) were first obtained by RT-PCR and DNA-seq in the present study. Sequence analyses showed that the <italic>TFAM</italic> gene contains a 741-bp CDS region encoding 246 amino acids sharing a 100% homology with the sequence on NCBI, while <italic>TFB2M</italic> gene contains a CDS region of 1176 bp encoding 391 amino acids with two missense mutations. The results of quantitative Real-Time PCR for <italic>TFAM</italic> and <italic>TFB2M</italic> revealed that transcripts of the genes were both presented at the highest levels in spleen tissue followed by liver tissue, while the least levels in <italic>longissimus dorsi</italic> muscle (LDM), and obviously the higher levels in two adipose tissues than those in LDM tissue (P&lt;0.01). Meanwhile, a total of forty-two JBPs were employed in this experiment to investigate the effect of these two genes on the carcass, meat quality traits and flavor substances such as fatty acids, intramuscular fat (IMF) in LDM. As expected, some strong correlations of gene expression abundance of <italic>TFAM</italic> and <italic>TFB2M</italic> mRNA in particular tissues such as liver and LDM with carcass and meat quality traits including marbling score, as well as the content of saturated fatty acid (SFA), in JBP were found.</p></abstract>

The coding sequences (CDS) of TFAM and TFB2M genes from Jiaxing Black Pig (JBP) were first obtained by RT-PCR and DNA-seq in the present study. Sequence analyses showed that the TFAM gene contains a 741-bp CDS region encoding 246 amino acids sharing a 100% homology with the sequence on NCBI, while TFB2M gene contains a CDS region of 1176 bp encoding 391 amino acids with two missense mutations. The results of quantitative Real-Time PCR for TFAM and TFB2M revealed that transcripts of the genes were both presented at the highest levels in spleen tissue followed by liver tissue, while the least levels in longissimus dorsi muscle (LDM), and obviously the higher levels in two adipose tissues than those in LDM tissue (P<0.01). Meanwhile, a total of forty-two JBPs were employed in this experiment to investigate the effect of these two genes on the carcass, meat quality traits and flavor substances such as fatty acids, intramuscular fat (IMF) in LDM. As expected, some strong correlations of gene expression abundance of TFAM and TFB2M mRNA in particular tissues such as liver and LDM with carcass and meat quality traits including marbling score, as well as the content of saturated fatty acid (SFA), in JBP were found.

Cloning and Expression Levels of <italic>TFAM</italic> and <italic>TFB2M</italic> Genes and their Correlation with Meat and Carcass Quality Traits in Jiaxing Black Pig

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cloning and Expression Levels of  and  Genes and their Correlation with Meat and Carcass Quality Traits in Jiaxing Black Pig

The coding sequences (CDS) of TFAM and TFB2M genes from Jiaxing Black Pig (JBP) were first obtained by RT-PCR and DNA-seq in the present study. Sequence...

Cloning and Expression Levels of TFAM and TFB2M Genes and their Correlation with Meat and Carcass Quality Traits in Jiaxing Black Pig

Published Online: May 02, 2019

Page range: 327 - 341

Received: Jul 27, 2018

Accepted: Nov 21, 2018

DOI: https://doi.org/10.2478/aoas-2018-0056

Keywords
cloning, Jiaxing Black Pig, and, mRNA expression, carcass and meat quality traits

© 2019 Qianqian Song et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cloning and Expression Levels of TFAM and TFB2M Genes and their Correlation with Meat and Carcass Quality Traits in Jiaxing Black Pig

Published Online: May 02, 2019

Page range: 327 - 341

Received: Jul 27, 2018

Accepted: Nov 21, 2018

DOI: https://doi.org/10.2478/aoas-2018-0056

Keywordscloning, Jiaxing Black Pig, and, mRNA expression, carcass and meat quality traits

© 2019 Qianqian Song et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cloning, Jiaxing Black Pig, and, mRNA expression, carcass and meat quality traits