<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The activity of calpains, in particular μ-calpain, is associated with several processes occurring in muscle tissue postmortem and influences meat quality parameters. Therefore, the <italic>CAPN1</italic> gene coding for large subunit of μ-calpain is considered as a candidate gene associated with meat quality traits. The aim of our study was to identify new polymorphisms in regulatory regions of the porcine <italic>CAPN1</italic> gene and to estimate their impact on <italic>CAPN1</italic> transcript abundance. In the present study, 7 polymorphisms in the porcine <italic>CAPN1</italic> gene were identified, of which 5 were localized in introns (g.1195_1197insCCT; g.1429G>A; g. [4479A>G; 4526A>T; 4529_4530delAG]), one in 3’ untranslated region (g.25676C>T) and one microsatellite sequence in promoter region (c.-155- AGGG [3_5]). The analysed populations (a total of 451 gilts representing three pure breeds: Pietrain, P lish Landrace, Polish Large White and one conservation breed Pu.awska) were not in Hardy-Weinberg equilibrium according to mutation g.25676C>T in 3’ untranslated region (all breeds), g.1429G>A (Pu.awska pigs) and g. [4479A>G; 4526A>T; 4529_4530delAG] (PLW pigs). Furthermore, the analysed SNPs in the porcine <italic>CAPN1</italic> gene were in linkage disequilibrium (P≤0.05). The <italic>CAPN1</italic> transcript abundance was also estimated in two important muscles (<italic>m. longissimus dorsi, m. semimembranosus</italic>). In <italic>longissimus dorsi</italic> muscle, a significant effect of c.-155- AGGG [3_5] polymorphism in promoter region on <italic>CAPN1</italic> expression levels was determined. The c.-155AGGG [3/3] pigs showed a statistically higher (P≤0.05) expression level of the <italic>CAPN1</italic> gene when compared to c.-155AGGG [4/4] homozygotes. The results obtained suggested that detected SNPs within regulatory regions of the <italic>CAPN1</italic> gene could be related to transcript level and activity of μ-calpain. The selected polymorphisms areproposed to be associated with meat production traits.</p></abstract>

The activity of calpains, in particular μ-calpain, is associated with several processes occurring in muscle tissue postmortem and influences meat quality parameters. Therefore, the CAPN1 gene coding for large subunit of μ-calpain is considered as a candidate gene associated with meat quality traits. The aim of our study was to identify new polymorphisms in regulatory regions of the porcine CAPN1 gene and to estimate their impact on CAPN1 transcript abundance. In the present study, 7 polymorphisms in the porcine CAPN1 gene were identified, of which 5 were localized in introns (g.1195_1197insCCT; g.1429G>A; g. [4479A>G; 4526A>T; 4529_4530delAG]), one in 3’ untranslated region (g.25676C>T) and one microsatellite sequence in promoter region (c.-155- AGGG [3_5]). The analysed populations (a total of 451 gilts representing three pure breeds: Pietrain, P lish Landrace, Polish Large White and one conservation breed Pu.awska) were not in Hardy-Weinberg equilibrium according to mutation g.25676C>T in 3’ untranslated region (all breeds), g.1429G>A (Pu.awska pigs) and g. [4479A>G; 4526A>T; 4529_4530delAG] (PLW pigs). Furthermore, the analysed SNPs in the porcine CAPN1 gene were in linkage disequilibrium (P≤0.05). The CAPN1 transcript abundance was also estimated in two important muscles (m. longissimus dorsi, m. semimembranosus). In longissimus dorsi muscle, a significant effect of c.-155- AGGG [3_5] polymorphism in promoter region on CAPN1 expression levels was determined. The c.-155AGGG [3/3] pigs showed a statistically higher (P≤0.05) expression level of the CAPN1 gene when compared to c.-155AGGG [4/4] homozygotes. The results obtained suggested that detected SNPs within regulatory regions of the CAPN1 gene could be related to transcript level and activity of μ-calpain. The selected polymorphisms areproposed to be associated with meat production traits.

New Polymorphisms in Regulatory Regions of Porcine μ-Calpain Gene and Their Association with <italic>CAPN1</italic> Transcript Abundance

Department of Animal Genetics and Breeding National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New Polymorphisms in Regulatory Regions of Porcine μ-Calpain Gene and Their Association with  Transcript Abundance

The activity of calpains, in particular μ-calpain, is associated with several processes occurring in muscle tissue postmortem and influences meat quality...

New Polymorphisms in Regulatory Regions of Porcine μ-Calpain Gene and Their Association with CAPN1 Transcript Abundance

Published Online: Jul 29, 2014

Page range: 525 - 535

Received: Oct 30, 2013

Accepted: Feb 18, 2014

DOI: https://doi.org/10.2478/aoas-2014-0027

Keywords
calpain, gene, polymorphism, expression level, pig

© 2014 Katarzyna Ropka-Molik et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New Polymorphisms in Regulatory Regions of Porcine μ-Calpain Gene and Their Association with CAPN1 Transcript Abundance

Published Online: Jul 29, 2014

Page range: 525 - 535

Received: Oct 30, 2013

Accepted: Feb 18, 2014

DOI: https://doi.org/10.2478/aoas-2014-0027

Keywordscalpain, gene, polymorphism, expression level, pig

© 2014 Katarzyna Ropka-Molik et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
calpain, gene, polymorphism, expression level, pig