<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Objectives</bold>: The aim of the study was to propose a new UHPLC method for the determination of cannabidiol (CBD) from supplements and drugs available on the Romanian market.<bold>Materials and methods</bold>: The HPLC assay of CBD was achieved by using a Phenomenex Gemini NX-C18 column. The mobile phase consisted of 70% acetonitrile and 30% water. Elution was performed in isocratic mode and the detection was done at 208 nm. The method was tested on hard capsules containing 150 mg of CBD.<bold>Results and discussions</bold>: The retention time of CBD was 2.8 minutes. Regression analysis showed good linearity over the 1-100 ug/ml concentration range. The lowest limit of quantification was established at 1 µg/ml. The method was developed by using reconstituted capsules. The substance proved low stability in solution at room temperature and stability at temperatures between 2-8°C. The recovery of reconstituted samples was 96.77%. The commercial capsules had a very low content of 15-20% from declared content.<bold>Conclusions</bold>: The proposed method can be used for CBD determination in different pharmaceutical formulations – hard and soft capsules with coconut oil as excipient.</abstract>

Objectives: The aim of the study was to propose a new UHPLC method for the determination of cannabidiol (CBD) from supplements and drugs available on the Romanian market.Materials and methods: The HPLC assay of CBD was achieved by using a Phenomenex Gemini NX-C18 column. The mobile phase consisted of 70% acetonitrile and 30% water. Elution was performed in isocratic mode and the detection was done at 208 nm. The method was tested on hard capsules containing 150 mg of CBD.Results and discussions: The retention time of CBD was 2.8 minutes. Regression analysis showed good linearity over the 1-100 ug/ml concentration range. The lowest limit of quantification was established at 1 µg/ml. The method was developed by using reconstituted capsules. The substance proved low stability in solution at room temperature and stability at temperatures between 2-8°C. The recovery of reconstituted samples was 96.77%. The commercial capsules had a very low content of 15-20% from declared content.Conclusions: The proposed method can be used for CBD determination in different pharmaceutical formulations – hard and soft capsules with coconut oil as excipient.

New UHPLC Method for Cannabidiol Determination in Hard Capsules

University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș

Acta Marisiensis - Seria Medica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"New UHPLC Method for Cannabidiol Determination in Hard Capsules"}

Objectives: The aim of the study was to propose a new UHPLC method for the determination of cannabidiol (CBD) from supplements and drugs available on the...

New UHPLC Method for Cannabidiol Determination in Hard Capsules

Published Online: Jul 04, 2019

Page range: 45 - 48

Received: Mar 05, 2019

Accepted: Apr 27, 2019

DOI: https://doi.org/10.2478/amma-2019-0007

Keywords
cannabidiol, UHPLC, assay, hard capsules

© 2019 Robert-Alexandru Vlad et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New UHPLC Method for Cannabidiol Determination in Hard Capsules

Published Online: Jul 04, 2019

Page range: 45 - 48

Received: Mar 05, 2019

Accepted: Apr 27, 2019

DOI: https://doi.org/10.2478/amma-2019-0007

Keywordscannabidiol, UHPLC, assay, hard capsules

© 2019 Robert-Alexandru Vlad et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cannabidiol, UHPLC, assay, hard capsules