Open Access

Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form

Semiha Erisen
Semiha Erisen
, 
Tülin Arasoğlu
Tülin Arasoğlu
, 
Banu Mansuroglu
Banu Mansuroglu
, 
İsmail Kocacaliskan
İsmail Kocacaliskan
 and 
Serap Derman
Serap Derman
   | Apr 09, 2020
Archives of Industrial Hygiene and Toxicology's Cover Image
About this article
Previous Article
Next Article
Cite
Article Category: Original article
Published Online: Apr 09, 2020
Page range: 69 - 77
Received: Oct 01, 2019
Accepted: Feb 01, 2020
DOI: https://doi.org/10.2478/aiht-2020-71-3344
© 2020 Semiha Erisen, Tülin Arasoğlu, Banu Mansuroglu, İsmail Kocacaliskan, Serap Derman, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Chemical structure of juglone (A) and poly (lactic-co-glycolic acid) (B)
Chemical structure of juglone (A) and poly (lactic-co-glycolic acid) (B)

Figure 2

Particle size distribution (A), scanning electron microscopy image (B), FT-IR spectra (C), and release pattern (D) of nanoparticles
Particle size distribution (A), scanning electron microscopy image (B), FT-IR spectra (C), and release pattern (D) of nanoparticles

Figure 3

Viability of L929 fibroblasts treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of quadruple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)
Viability of L929 fibroblasts treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of quadruple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)

Figure 4

Viability of alfalfa cells treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of triple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)
Viability of alfalfa cells treated with free and PLGA nanoparticle-coated juglone (JNP) after 24 h (A) and 48 h (B); Cell viability was measured with the MTT assay. Values are expressed as means of triple samples with standard error. *statistically significant differences between juglone and JNP (p<0.05)

Figure 5

Potential mutagenicity in S. typhimurium TA98 treated with 0.31−10 μmol/L juglone: (A) TA98-S9 treated with JNPs; (B) TA98+S9 treated with JNPs; (C) TA98-S9 treated with free juglone; (D) TA98+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (1 μg/mL) with S9; NC – negative control: water; *B value ≥0.99
Potential mutagenicity in S. typhimurium TA98 treated with 0.31−10 μmol/L juglone: (A) TA98-S9 treated with JNPs; (B) TA98+S9 treated with JNPs; (C) TA98-S9 treated with free juglone; (D) TA98+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (1 μg/mL) with S9; NC – negative control: water; *B value ≥0.99

Figure 6

Potential mutagenicity in S. typhimurium TA100 treated with 0.31−10 μmol/L juglone: (A) TA100-S9 treated with JNPs; (B) TA100+S9 treated with JNPs; (C) TA100-S9 treated with free juglone; (D) TA100+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (2.5 μg/mL) with S9; NC – negative control: water; *B value ≥0.99
Potential mutagenicity in S. typhimurium TA100 treated with 0.31−10 μmol/L juglone: (A) TA100-S9 treated with JNPs; (B) TA100+S9 treated with JNPs; (C) TA100-S9 treated with free juglone; (D) TA100+S9 treated with free juglone; PC – positive control: 2-NF (2 μg/mL) and 4-NQO (0.1 μg/mL) without S9 and 2-AA (2.5 μg/mL) with S9; NC – negative control: water; *B value ≥0.99

Physicochemical properties of juglone loaded PLGA nanoparticles

Encapsulating efficiency (%) Drug loading (%) Particle size (nm) Zeta potential (mV) Polydispersity index
29.95±2.8 15.42±2.1 213.33±5.2 -14.58±2.8 0.115±0.03
eISSN:
1848-6312
Languages:
English, Slovenian
Publication timeframe:
4 times per year
Journal Subjects:
Medicine, Basic Medical Science, other
Journal RSS Feed