<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Application of sewage sludge to soil is a potentially inexpensive source of nutrition for plants, but may contain undesirable and toxic substances, e.g. heavy metals. Alterations in microbial communities can serve as an environmental indicator of possible soil contamination. We used two molecular fingerprinting methods (Automated Ribosomal Intergenic Spacer Analysis, ARISA and Terminal Restriction Fragment Length Polymorphism, T-RFLP) to study changes in the genetic diversity of bacterial communities in the rhizosphere of <italic>Arundo donax</italic> L. cultivated in the soil fertilised with additive based on sewage sludge from wastewater treatment plant and agricultural by-products represented by crushed corn hobs and wastes from grain mill industry. The metagenomic DNA extracted from rhizosphere samples were collected in August and November 2014. The amount of mgDNA was statistically higher in samples with additive than in control samples without it in both dates. The Venn diagrams showed that operational taxonomic units which were common to all samples were represented in 32.8% in ARISA and 43.4% in T-RFLP. However, based on Principal component analysis and subsequent PERMANOVA statistical tests did not confirm significant differences in the rhizosphere of control plants and plants grown in the soil supplemented with sewage sludge in dose 5 and 15 t/ha present in the additive.</p></abstract>

Application of sewage sludge to soil is a potentially inexpensive source of nutrition for plants, but may contain undesirable and toxic substances, e.g. heavy metals. Alterations in microbial communities can serve as an environmental indicator of possible soil contamination. We used two molecular fingerprinting methods (Automated Ribosomal Intergenic Spacer Analysis, ARISA and Terminal Restriction Fragment Length Polymorphism, T-RFLP) to study changes in the genetic diversity of bacterial communities in the rhizosphere of Arundo donax L. cultivated in the soil fertilised with additive based on sewage sludge from wastewater treatment plant and agricultural by-products represented by crushed corn hobs and wastes from grain mill industry. The metagenomic DNA extracted from rhizosphere samples were collected in August and November 2014. The amount of mgDNA was statistically higher in samples with additive than in control samples without it in both dates. The Venn diagrams showed that operational taxonomic units which were common to all samples were represented in 32.8% in ARISA and 43.4% in T-RFLP. However, based on Principal component analysis and subsequent PERMANOVA statistical tests did not confirm significant differences in the rhizosphere of control plants and plants grown in the soil supplemented with sewage sludge in dose 5 and 15 t/ha present in the additive.

Rhizosphere Bacterial Communities of <italic>Arundo Donax</italic> Grown in Soil Fertilised with Sewage Sludge and Agricultural by-Products

National Agricultural and Food Centre – Research Institute of Plant Production

Agriculture (Pol'nohospodárstvo)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Rhizosphere Bacterial Communities of  Grown in Soil Fertilised with Sewage Sludge and Agricultural by-Products

Application of sewage sludge to soil is a potentially inexpensive source of nutrition for plants, but may contain undesirable and toxic substances, e.g. heavy...

Rhizosphere Bacterial Communities of Arundo Donax Grown in Soil Fertilised with Sewage Sludge and Agricultural by-Products

Published Online: May 09, 2019

Page range: 37 - 41

Received: Dec 11, 2018

Accepted: Apr 02, 2019

DOI: https://doi.org/10.2478/agri-2019-0004

Keywords
genetic diversity, ARISA, T-RFLP, L

© 2019 Katarína Ondreičková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Rhizosphere Bacterial Communities of Arundo Donax Grown in Soil Fertilised with Sewage Sludge and Agricultural by-Products

Published Online: May 09, 2019

Page range: 37 - 41

Received: Dec 11, 2018

Accepted: Apr 02, 2019

DOI: https://doi.org/10.2478/agri-2019-0004

Keywordsgenetic diversity, ARISA, T-RFLP, L

© 2019 Katarína Ondreičková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
genetic diversity, ARISA, T-RFLP, L