<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Wheat is one of three most important cereals worldwide. Its production rises every year. There is a possibility to improve quantitative and qualitative parameters by biolistic method of transformation. The process of introduction of desired gene into the wheat genome and plant regeneration is affected by many factors. To identify the suitable conditions, selection system, the influence of donor, plant environment and the regeneration capacity of wheat genotypes were tested. The scutella of immature embryos served as the initial explants. Plant regeneration was achieved by 6 out of 11 genotypes tested. The highest values were reached by the cultivar Ilias. The effect of donor, plant environment was evaluated by two genotypes. Plants from growth chamber appeared to be better source of explants compared to plants grown in the natural conditions. The selection system was optimized as follows: regeneration medium in the dark and subsequently in photoperiod conditions (DR) with 5 mg/l of phosphinotricin (PPT), shoot induction medium (DS) with 7 mg/l of PPT.</p></abstract>

Wheat is one of three most important cereals worldwide. Its production rises every year. There is a possibility to improve quantitative and qualitative parameters by biolistic method of transformation. The process of introduction of desired gene into the wheat genome and plant regeneration is affected by many factors. To identify the suitable conditions, selection system, the influence of donor, plant environment and the regeneration capacity of wheat genotypes were tested. The scutella of immature embryos served as the initial explants. Plant regeneration was achieved by 6 out of 11 genotypes tested. The highest values were reached by the cultivar Ilias. The effect of donor, plant environment was evaluated by two genotypes. Plants from growth chamber appeared to be better source of explants compared to plants grown in the natural conditions. The selection system was optimized as follows: regeneration medium in the dark and subsequently in photoperiod conditions (DR) with 5 mg/l of phosphinotricin (PPT), shoot induction medium (DS) with 7 mg/l of PPT.

The Choice of Suitable Conditions for Wheat Genetic Transformation

National Agricultural and Food Centre – Research Institute of Plant Production

Agriculture (Pol'nohospodárstvo)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"The Choice of Suitable Conditions for Wheat Genetic Transformation"}

Wheat is one of three most important cereals worldwide. Its production rises every year. There is a possibility to improve quantitative and qualitative...

The Choice of Suitable Conditions for Wheat Genetic Transformation

Published Online: May 09, 2019

Page range: 30 - 36

Received: Sep 12, 2018

Accepted: Apr 02, 2019

DOI: https://doi.org/10.2478/agri-2019-0003

Keywords
wheat ( L.), genotype, selection, biolistic transformation, donor plant

© 2019 Lenka Klčová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Choice of Suitable Conditions for Wheat Genetic Transformation

Published Online: May 09, 2019

Page range: 30 - 36

Received: Sep 12, 2018

Accepted: Apr 02, 2019

DOI: https://doi.org/10.2478/agri-2019-0003

Keywordswheat ( L.), genotype, selection, biolistic transformation, donor plant

© 2019 Lenka Klčová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
wheat ( L.), genotype, selection, biolistic transformation, donor plant