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European Pharmaceutical Journal
Volume 67 (2020): Issue 1 (January 2020)
Open Access
Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells
in vitro
G. Hrčková
G. Hrčková
,
T. Mačák Kubašková
T. Mačák Kubašková
,
D. Mudroňová
D. Mudroňová
and
A. Bardelčíková
A. Bardelčíková
| Nov 18, 2020
European Pharmaceutical Journal
Volume 67 (2020): Issue 1 (January 2020)
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Article Category:
Original research article/Review
Published Online:
Nov 18, 2020
Page range:
17 - 26
Received:
Oct 02, 2019
Accepted:
Dec 10, 2019
DOI:
https://doi.org/10.2478/afpuc-2020-0003
Keywords
silymarin
,
mouse
,
splenocytes
,
proliferation
,
mitochondrial potential
,
apoptosis
© 2019 Hrčková G et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
The effect of silymarin on CoA-activated mouse spleen cells in vitro: (a) proliferation index of T lymphocytes; (b) viability test of T lymphocytes using trypan blue staining. Significantly different values between CoA alone and CoA + silymarin treated groups are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 2
The effect of silymarin on cytokine secretion and mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro: (a) concentration of IFN-γ and (b) IL-4 in supernatants of cells after 70 h cultivation; (c) relative gene expression for IFN-γ and (d) IL-4 in T lymphocytes after 70 h of cultivation. Significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 3
The effect of silymarin on mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro for (a) transcription factor NF-κB and (b) transcription factor Foxp3. Significantly different values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 4
The effect of silymarin on mitochondrial membrane potential in naïve and CoA-activated mouse spleen cells and after treatment with silymarin (b), expressed as the mean intensity of fluorescence (MIF) for Rhodamine 123: (a) representative plots of MIF for control and individual silymarin-treated groups. Significantly different values are indicated as: *p < 0.05.
Figure 5
The proportions of live, early apoptotic, and late apoptotic/dead cells determined for the suspensions of naïve splenocytes, CoA-activated T lymphocytes, and following in vitro treatment with silymarin after 70 h of cultivation (b). Representative flow cytometric dot plots showing Annexin V and propidium iodide staining of naïve splenocytes and CoA-activated cells following silymarin treatment (a). Significantly different values between CoA alone and silymarin-treated groups for early apoptotic cells are indicated as *p < 0.05 and for live cells are indicated as Ñp < 0.05.
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