<abstract xmlns="http://www.w3.org/1999/xhtml">In this investigation, the genotoxic properties of mestranol were examined <italic>in vitro</italic>. Human lymphocyte cultures were exposed for 72 h to mestranol at concentrations of 7.5, 15 and 30 µg/g. The genotoxic effects of the chemosterilant were assessed by numerical and structural chromosome aberrations.Mestranol induced certain genotoxic effects in human lymphocytes. There was a dose-dependent significant (p&lt;0.01) increase in the number of numerical aberrations in comparison to the control, but without significant differences (p>0.05) between the doses applied. Further, structural aberrations increased significantly (p&lt;0.01) in the presence of mestranol, being most frequent in cultures exposed to the highest mestranol dose.The frequency of Robertsonian translocations increased significantly only in cultures treated with mestranol at concentration of 30 µg/g in comparison both with the control (p&lt;0.01) and the lowest chemosterilant dose (p&lt;0.01).There were significant differences (p&lt;0.01) in the levels of chromosome gaps and fragments compared to Robertsonian translocations, whilst the frequencies between gaps and fragments were not significantly different (p>0.05).</abstract>

In this investigation, the genotoxic properties of mestranol were examined in vitro. Human lymphocyte cultures were exposed for 72 h to mestranol at concentrations of 7.5, 15 and 30 µg/g. The genotoxic effects of the chemosterilant were assessed by numerical and structural chromosome aberrations.Mestranol induced certain genotoxic effects in human lymphocytes. There was a dose-dependent significant (p<0.01) increase in the number of numerical aberrations in comparison to the control, but without significant differences (p>0.05) between the doses applied. Further, structural aberrations increased significantly (p<0.01) in the presence of mestranol, being most frequent in cultures exposed to the highest mestranol dose.The frequency of Robertsonian translocations increased significantly only in cultures treated with mestranol at concentration of 30 µg/g in comparison both with the control (p<0.01) and the lowest chemosterilant dose (p<0.01).There were significant differences (p<0.01) in the levels of chromosome gaps and fragments compared to Robertsonian translocations, whilst the frequencies between gaps and fragments were not significantly different (p>0.05).

Chromosome Aberrations Produced by Mestranol in Human Lymphocyte Cultures

Department of Animal Hygiene, Faculty of Veterinary Medicine

Department of Economics and Statistics, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Chromosome Aberrations Produced by Mestranol in Human Lymphocyte Cultures"}

In this investigation, the genotoxic properties of mestranol were examined in vitro. Human lymphocyte cultures were exposed for 72 h to mestranol at concentrations of 7.5, 15 and 30 µg/g. The genotoxic effects of the chemosterilant were assessed by numerical and structural chromosome aberrations.Mestranol induced certain genotoxic effects in human lymphocytes. There was a dose-dependent significant (p0.05) between the doses applied. Further, structural aberrations increased significantly (p0.05).

In this investigation, the genotoxic properties of mestranol were examined in vitro. Human lymphocyte cultures were exposed for 72 h to mestranol at...

Chromosome Aberrations Produced by Mestranol in Human Lymphocyte Cultures

Published Online: Dec 21, 2019

Page range: 426 - 433

Received: Jul 10, 2019

Accepted: Oct 29, 2019

DOI: https://doi.org/10.2478/acve-2019-0036

Keywords
mestranol, cytogenetics, genotoxicity, human lymphocytes

© 2019 Teodorović Radislava et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Chromosome Aberrations Produced by Mestranol in Human Lymphocyte Cultures

Published Online: Dec 21, 2019

Page range: 426 - 433

Received: Jul 10, 2019

Accepted: Oct 29, 2019

DOI: https://doi.org/10.2478/acve-2019-0036

Keywordsmestranol, cytogenetics, genotoxicity, human lymphocytes

© 2019 Teodorović Radislava et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
mestranol, cytogenetics, genotoxicity, human lymphocytes