<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and these were further analyzed by restriction fragment length polymorphism (RFLP) and DNA sequence analysis. A high prevalence of the CPV-2c variant (79.56% of CPV-2 positive dogs) was observed in this survey, underlining that this type of CPV field isolate is prevalent circulating in Sicily. Sequence and phylogenetic analysis showed a close relationship with CPV-2a and CPV-2c strains from Europe and non-European countries. Considering that CPV-2c is reaching a worldwide distribution and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new CPV vaccines.</p></abstract>

Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and these were further analyzed by restriction fragment length polymorphism (RFLP) and DNA sequence analysis. A high prevalence of the CPV-2c variant (79.56% of CPV-2 positive dogs) was observed in this survey, underlining that this type of CPV field isolate is prevalent circulating in Sicily. Sequence and phylogenetic analysis showed a close relationship with CPV-2a and CPV-2c strains from Europe and non-European countries. Considering that CPV-2c is reaching a worldwide distribution and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new CPV vaccines.

Investigation on <italic>Canine parvovirus</italic> circulation in dogs from Sicily (Italy) by biomolecular assay

Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation on  circulation in dogs from Sicily (Italy) by biomolecular assay

Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2...

Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay

Published Online: Mar 27, 2018

Page range: 80 - 94

Received: Mar 07, 2017

Accepted: Dec 25, 2017

DOI: https://doi.org/10.2478/acve-2018-0007

Keywords
polymerase chain reaction, canine parvovirus, virus isolation

© 2018 Purpari Giuseppa et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay

Published Online: Mar 27, 2018

Page range: 80 - 94

Received: Mar 07, 2017

Accepted: Dec 25, 2017

DOI: https://doi.org/10.2478/acve-2018-0007

Keywordspolymerase chain reaction, canine parvovirus, virus isolation

© 2018 Purpari Giuseppa et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
polymerase chain reaction, canine parvovirus, virus isolation