<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Adrenaline is a neurotransmitter and hormone that plays an important role in physiological regulatory mechanisms. The objective of this study was to assess primary DNA damage in isolated human lymphocytes exposed to adrenaline using the in vitro comet assay. Dose-response of human lymphocytes was determined at concentration range of adrenaline from 0.01 μM to 300 μM for various treatment times (1h, 2h, 4h and 24h). The obtained results showed that adrenaline induced DNA damage at concentration range from 5 μM to 300 μM after 1h, 2h and 4h of treatment. The slightest DNA damage was observed after 24 h of adrenaline treatment - only the highest concentrations of adrenaline (150 μM and 300 μM) caused increased level of DNA damage. In order to evaluate the potential contribution of reactive oxygen species (ROS) in adrenaline-induced DNA damage we used antioxidants catalase (100 IU/mL and 500 IU/mL) and quercetin (100 μM and 500 μM). Co-treatment of lymphocytes with adrenaline (300 μM) and antioxidants for 1 h, significantly reduced the quantity of DNA in the comet tails. Therefore, it can be concluded that adrenaline exhibits genotoxic effects mainly through induction of reactive oxygen species and that some of the DNA damage is repaired during the first four hours following the treatment with adrenaline.</p></abstract>

Adrenaline is a neurotransmitter and hormone that plays an important role in physiological regulatory mechanisms. The objective of this study was to assess primary DNA damage in isolated human lymphocytes exposed to adrenaline using the in vitro comet assay. Dose-response of human lymphocytes was determined at concentration range of adrenaline from 0.01 μM to 300 μM for various treatment times (1h, 2h, 4h and 24h). The obtained results showed that adrenaline induced DNA damage at concentration range from 5 μM to 300 μM after 1h, 2h and 4h of treatment. The slightest DNA damage was observed after 24 h of adrenaline treatment - only the highest concentrations of adrenaline (150 μM and 300 μM) caused increased level of DNA damage. In order to evaluate the potential contribution of reactive oxygen species (ROS) in adrenaline-induced DNA damage we used antioxidants catalase (100 IU/mL and 500 IU/mL) and quercetin (100 μM and 500 μM). Co-treatment of lymphocytes with adrenaline (300 μM) and antioxidants for 1 h, significantly reduced the quantity of DNA in the comet tails. Therefore, it can be concluded that adrenaline exhibits genotoxic effects mainly through induction of reactive oxygen species and that some of the DNA damage is repaired during the first four hours following the treatment with adrenaline.

The Investigation of DNA Damage Induced by Adrenaline in Human Lymphocytes in Vitro/Ispitivanja Oštećenja DNK Izazvanih Adrenalinom U Limfocitima Čoveka in Vitro

Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia

Serbian Academy of Sciences and Arts, Knez Mihailova 35, Belgrade, Serbia

Department of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, Serbia

School of Medicine, University of Niš, Bul. dr Zorana Djindjica 81, Niš, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Adrenaline is a neurotransmitter and hormone that plays an important role in physiological regulatory mechanisms. The objective of this study was to assess...

The Investigation of DNA Damage Induced by Adrenaline in Human Lymphocytes in Vitro/Ispitivanja Oštećenja DNK Izazvanih Adrenalinom U Limfocitima Čoveka in Vitro

Published Online: Oct 03, 2014

Page range: 281 - 292

Received: May 09, 2014

Accepted: Aug 24, 2014

DOI: https://doi.org/10.2478/acve-2014-0027

Keywords
adrenaline, antioxidants, comet assay, human lymphocytes

© by Radaković Milena

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Investigation of DNA Damage Induced by Adrenaline in Human Lymphocytes in Vitro/Ispitivanja Oštećenja DNK Izazvanih Adrenalinom U Limfocitima Čoveka in Vitro

Published Online: Oct 03, 2014

Page range: 281 - 292

Received: May 09, 2014

Accepted: Aug 24, 2014

DOI: https://doi.org/10.2478/acve-2014-0027

Keywordsadrenaline, antioxidants, comet assay, human lymphocytes

© by Radaković Milena

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
adrenaline, antioxidants, comet assay, human lymphocytes