<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromatographic separation material. Its equilibrium properties were investigated in batch adsorption experiments. The effect of pH in the range of 5.5—7.5 and NaCl concentration in the range of 0—300 mM on the adsorption of rhEPO and contaminant proteins was examined. Optimal conditions found in these equilibrium experiments were applied to rhEPO adsorption in a chromatographic column. Several experiments were carried out at different elution conditions to optimize the rhEPO yield and selectivity.</p></abstract>

This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromatographic separation material. Its equilibrium properties were investigated in batch adsorption experiments. The effect of pH in the range of 5.5—7.5 and NaCl concentration in the range of 0—300 mM on the adsorption of rhEPO and contaminant proteins was examined. Optimal conditions found in these equilibrium experiments were applied to rhEPO adsorption in a chromatographic column. Several experiments were carried out at different elution conditions to optimize the rhEPO yield and selectivity.

Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent

Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant....

Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent

Published Online: Jul 09, 2019

Page range: 103 - 107

DOI: https://doi.org/10.2478/acs-2019-0015

Keywords
erythropoietin, multimodal adsorbent, selectivity, elution chromatography

© 2019 Marta Ostrihoňová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent

Published Online: Jul 09, 2019

Page range: 103 - 107

DOI: https://doi.org/10.2478/acs-2019-0015

Keywordserythropoietin, multimodal adsorbent, selectivity, elution chromatography

© 2019 Marta Ostrihoňová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
erythropoietin, multimodal adsorbent, selectivity, elution chromatography