<abstract xmlns="http://www.w3.org/1999/xhtml">The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification (<italic>LOD</italic> and <italic>LOQ</italic>) of meloxicam by TLC-densitometric technique was estimated. Of all chromatographic conditions tested, the lowest limiting values, thus the best sensitivity, in the NP-TLC system was achieved on silica gel 60F254 and neutral aluminum oxide plates developed with the mobile phase consisting of ethyl acetate/toluene/<italic>n</italic>-butylamine (2:2:1, <italic>V</italic>/<italic>V</italic>/<italic>V</italic>). In the case of the RP-TLC method, a mixture of methanol/water (8:2, <italic>V</italic>/<italic>V</italic>) enabled densitometric detection of meloxicam at the lowest concentration level on RP-8F254 and RP-18F254 plates. Additionally, the smallest <italic>LOD</italic> value of meloxicam ensured crystalline violet and gentian violet as visualization agents on silica gel 60F254 and neutral aluminum oxide 150F254 plates, resp. Comparison of the densitometrically obtained spectra of meloxicam drug and its standard after the use of appropriate visualization agents could be a good and cheap alternative tool for the identification of meloxicam as an active pharmaceutical ingredient.</abstract>

The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification (LOD and LOQ) of meloxicam by TLC-densitometric technique was estimated. Of all chromatographic conditions tested, the lowest limiting values, thus the best sensitivity, in the NP-TLC system was achieved on silica gel 60F254 and neutral aluminum oxide plates developed with the mobile phase consisting of ethyl acetate/toluene/n-butylamine (2:2:1, V/V/V). In the case of the RP-TLC method, a mixture of methanol/water (8:2, V/V) enabled densitometric detection of meloxicam at the lowest concentration level on RP-8F254 and RP-18F254 plates. Additionally, the smallest LOD value of meloxicam ensured crystalline violet and gentian violet as visualization agents on silica gel 60F254 and neutral aluminum oxide 150F254 plates, resp. Comparison of the densitometrically obtained spectra of meloxicam drug and its standard after the use of appropriate visualization agents could be a good and cheap alternative tool for the identification of meloxicam as an active pharmaceutical ingredient.

A cost-effective and sensitive TLC-densitometric identification of meloxicam

Department of Analytical Chemistry Faculty of Pharmaceutical Sciences in Sosnowiec

Medical University of Silesia in Katowice

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"A cost-effective and sensitive TLC-densitometric identification of meloxicam"}

The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification...

A cost-effective and sensitive TLC-densitometric identification of meloxicam

Published Online: Jul 20, 2020

Page range: 143 - 152

Accepted: Mar 24, 2020

DOI: https://doi.org/10.2478/acph-2021-0006

Keywords
meloxicam, TLC-densitometry, identification, sensitivity, visualization agents

© 2021 Wioletta Parys et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A cost-effective and sensitive TLC-densitometric identification of meloxicam

Published Online: Jul 20, 2020

Page range: 143 - 152

Accepted: Mar 24, 2020

DOI: https://doi.org/10.2478/acph-2021-0006

Keywordsmeloxicam, TLC-densitometry, identification, sensitivity, visualization agents

© 2021 Wioletta Parys et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
meloxicam, TLC-densitometry, identification, sensitivity, visualization agents