Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes

1. O. Lee and B. Lee, Antioxidant and antimicrobial activities of individual and combined phenolics in Olea europaea leaf extract, Bioresour. Technol.101 (2010) 3751–3754; https://doi.org/10.1016/j.biortech.2009.12.05210.1016/j.biortech.2009.12.052Search in Google Scholar

2. N. Zorić, I. Horvat, N. Kopjar, A. Vučemilović, D. Kremer, S. Tomić and I. Kosalec, Hydroxytyrosol expresses antifungal activity in vitro, Curr. Drug Targets14 (2013) 992–998.10.2174/13894501113149990167Search in Google Scholar

3. S. Cicerale, L. J. Lucas and R. S. J. Keast, Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil, Curr. Opin. Biotechnol.23 (2011) 1–7; https://doi.org/10.1016/j.copbio.2011.09.00610.1016/j.copbio.2011.09.006Search in Google Scholar

4. A. Aherne and N. M. O’Brien, Protection by the flavonoid myricetin, quercetin, and rutin against hydrogen peroxide-induced DNA damage in Caco-2 and Hep G2 cells, Nutr. Cancer34 (1999) 160–166; https://doi.org/10.1207/S15327914NC3402_610.1207/S15327914NC3402_6Search in Google Scholar

5. B. Uttara, A. V. Singh, P. Zamboni and R. T. Mahajan, Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options, Curr. Neuropharmacol.7 (2009) 65–74; https://doi.org/10.2174/15701590978760282310.2174/157015909787602823Search in Google Scholar

6. J. M. Mates and F. M. Sanchez-Jimenez, Role of reactive oxygen species in apoptosis: Implications for cancer therapy, Int. J. Biochem. Cell Biol.32 (2000) 157–170; https://doi.org/10.1016/S1357-2725(99)00088-610.1016/S1357-2725(99)00088-6Search in Google Scholar

7. P. Xie, L. Huang, C. Zhang and Y. Zhang, Phenolic compositions, and antioxidant performance of olive leaf and fruit (Olea europaea L.) extracts and their structure-activity relationship, J. Funct. Foods16 (2015) 460–471; https://doi.org/10.1016/j.jff.2015.05.00510.1016/j.jff.2015.05.005Search in Google Scholar

8. E. Gimeno, K. de la Torre-Carbot, R. M. Lamuela-Raventos, A. I. Castellote, M. Fito, R. de la Torre, M. I. Covas and M. Carmen Lopez-Sabater, Changes in the phenolic content of low density lipo-protein after olive oil consumption in men. A randomized crossover controlled trial, Br. J. Nutr.98 (2007) 1243–1250; https://doi.org/10.1017/S000711450777869810.1017/S000711450777869817617938Search in Google Scholar

9. S. Salvini, F. Sera, D. Caruso, L. Giovannelli, F. Visioli, C. Saieva, G. Masal, M. Ceroti, V. Giovacchini, V. Pitozzi, C. Galli, A. Romani, N. Mulinacci, R. Bortolomeazzi, P. Dolara and D. Palli, Daily consumption of a high-phenol extra-virgin olive oil reduces oxidative DNA damage in postmenopausal women, Br. J. Nutr.95 (2006) 742–751; https://doi.org/10.1079/BJN2005167410.1079/BJN2005167416571154Search in Google Scholar

10. A. Machowetz, H. E. Poulsen, S. Gruendel, A. Weimann, M. Fito, J. Marrugat, R. de la Torre, J. T. Salonen, K. Nyyssonen, J. Mursu, S. Nascetti, A. Gaddi, H. Kiesewetter, H. Baumler, H. Selmi, J. Kaikkonen, H. J. Zunft, M. I. Covas and C. Koebnick, Effect of olive oils on biomarkers of oxidative DNA stress in Northern and Southern Europeans, FASEB J.21 (2007) 45–52; https://doi.org/10.1096/fj.06-6328com10.1096/fj.06-6328com17110467Search in Google Scholar

11. S. Grasso, L. Siracusa, C. Spatafora, M. Renis and C. Tringali, Hydroxytyrosol lipophilic analogues: enzymathic sythesis, radical-scavenging activity and DNA oxidative damage protection, Bioorg. Chem.35 (2007) 137–152; https://doi.org/10.1016/j.bioorg.2006.09.00310.1016/j.bioorg.2006.09.003Search in Google Scholar

12. J. Anter, Z. Fernandez-Bedmar, M. Villatoro-Pulido, S. Demyda-Peyras, M. Moreno-Millan, A. Alonso-Moraga, A. Munoz-Serrano and M. D. Luque de Castro, A pilot study on the DNA-protective, cytotoxic, and apoptosis-inducing properties of olive-leaf extracts, Mutat. Res.723 (2011) 165–170; https://doi.org/10.1016/j.mrgentox.2011.05.00510.1016/j.mrgentox.2011.05.005Search in Google Scholar

13. E. M. Odiatou, A. L. Skaltsounis and A. I. Constantinou, Identification of the factors responsible for the in vitro proxidant and cytotoxic activities of the olive polyphenols oleuropein and hydroxytyrosol, Cancer Lett.330 (2013) 113–121; http://doi.org/10.1016/j.canlet.2012.11.03510.1016/j.canlet.2012.11.035Search in Google Scholar

14. R. Fabiani, P. Rosignoli, A. De Bartolomeo, R. Fuccelli, M. Servili, G. F. Montedoro and G. Morozzi, Oxidative DNA damage is prevented by extracts of olive oil, hydroxytyrosol, and other olive phenolic compounds in human blood mononuclear cells and HL60 cells, J. Nutr.138 (2008) 1411–1416; https://doi.org/10.1093/jn/138.8.141110.1093/jn/138.8.1411Search in Google Scholar

15. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation delorization assay, Free Radic. Biol. Med.26 (1999) 1231–1237; https://doi.org/10.1016/s0891-5849(98)00315-310.1016/S0891-5849(98)00315-3Search in Google Scholar

16. M. N. Asghar, I. U. Khan, I. Zia, M. Ahmad and F. A. Qureshi, Modified 2,2’-azinobis(3-ethylbenzo thiazoline)-6-sulphonic acid radical cation decolorization assay for antioxidant activity of human plasma and extracts of traditional medicinal plants, Acta Chim. Slov.55 (2008) 408–418.Search in Google Scholar

17. I. F. Benzie and J. J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of „antioxidant power“: the FRAP assay, Anal. Biochem.239 (1996) 70–76; https://doi.org/10.1006/abio.1996.029210.1006/abio.1996.0292Search in Google Scholar

18. R. Apak, K. Guclu, M. Ozyurek and S. E. Karademir, Novel total antioxidant index for dietary polyphenols and vitamin C and E using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, J. Agric. Food Chem.52 (2004) 7970–7981; https://doi.org/10.1021/jf048741x10.1021/jf048741xSearch in Google Scholar

19. N. P. Singh, M. T. McCoy, R. R. Tice and E. L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res.175 (1988) 184–191; https://doi.org/10.1016/0014-4827(88)90265-010.1016/0014-4827(88)90265-0Search in Google Scholar

20. W. Bors, W. Heller, C. Michel and M. Saran, Flavonoids as antioxidants: Determination of radical-scavenging efficiencies, Methods Enzymol.186 (1990) 343–355; https://doi.org/10.1016/0076-6879(90)86128-I10.1016/0076-6879(90)86128-ISearch in Google Scholar

21. O. Benavente-Garcia, J. Castillo, J. Lorente, A. Ortuno and J. A. Del Rio, Antioxidant activity of phenolics extracted from Olea europaea L. leaves, Food Chem. 68 (2000) 457–462; https://doi.org/10.1016/S0308-8146(99)00221-610.1016/S0308-8146(99)00221-6Search in Google Scholar

22. O. Benavente-Garcia, J. Castillo, F. R. Marin, A. Ortuno and J. A. del Rio, Uses and properties of Citrus flavonoids, J. Agric. Food Chem.45 (1997) 4504–4515; https://doi.org/10.1021/jf970373s10.1021/jf970373sSearch in Google Scholar

23. Z. Xiang and Z. Ning, Scavenging and antioxidant properties of compound derived from chlorogenic acid in South-China honeysuckle, LWT-Food Sci. Technol.41 (2008) 1189–1203; https://doi.org/10.1016/j.lwt.2007.08.00610.1016/j.lwt.2007.08.006Search in Google Scholar

24. M. Özyürek, K. Guclu and R. Apak, The main and modified CUPRAC methods of antioxidant measurement, Trends Anal. Chem.30 (2011) 652–664; https://doi.org/10.1016/j.trac.2010.11.01610.1016/j.trac.2010.11.016Search in Google Scholar

25. A. Lucić Vrdoljak, S. Žunec, B. Radić, R. Fuchs, D. Želježić and N. Kopjar, Evaluation of the cyto/genotoxicity profile of oxime K048 using human peripheral blood lymphocytes: An introductory study, Toxicol. In Vitro28 (2014) 39–45; https://doi.org/10.1016/j.tiv.2013.06.00710.1016/j.tiv.2013.06.007Search in Google Scholar

26. A. Vulić, K. Durgo, J. Pleadin, L. Herceg and N. Kopjar, Mutagenicity and DNA-damaging potential of clenbuterol and its metabolite 4-amino-3,5-dichlorobenzoic acid in vitro, Food Chem. Toxicol.77 (2015) 82–92; https://doi.org/10.1016/j.fct.2014.12.02310.1016/j.fct.2014.12.023Search in Google Scholar

27. S. J. Duthie, A. R. Collins, G. G. Duthie and V. L. Dobson, Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes, Mutat Res.393 (1997) 223–231; https://doi.org/10.1016/s1383-5718(97)00107-110.1016/S1383-5718(97)00107-1Search in Google Scholar

28. M. Czerwinska, A. K. Kiss and M. Naruszewicz, A comparison of antioxidant activity of oleuropein and its dialdehydic form oleacein, Food Chem.131 (2012) 953–960; https://doi.org/10.1016/j.foodchem.2011.09.08210.1016/j.foodchem.2011.09.082Search in Google Scholar

29. S. Bulotta, R. Corradino, M. Celano, M. D’Agostino, J. Maiuolo, M. Oliverio, A. Procopio, M. Ian-none, D. Rotiroti and D. Russo, Antiproliferative and antioxidant effects of oleuropein and its semisynthetic peracetylated derivatives on breast cancer cells, Food Chem.127 (2011) 1609–1614; https://doi.org/10.1016/j.foodchem.2011.02.02510.1016/j.foodchem.2011.02.025Search in Google Scholar

30. A. Čabarkapa, L. Živković, D. Žukovec, N. Djelić, V. Bajić, D. Dekanski and B. Spremo-Potparević, Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes, Toxicol. In Vitro28 (2014) 451–456; https://doi.org/10.1016/j.tiv.2013.12.01410.1016/j.tiv.2013.12.01424389114Search in Google Scholar

31. K. Kyriakopoulou, E. Katsanou, K. Machera and A. Charistou, Oleuropein protects Hep G2 cells against oxidative stress-induced DNA damage, Toxicol Lett.211 (2012) S106; https://doi.org/10.1016/j.toxlet.2012.03.39610.1016/j.toxlet.2012.03.396Search in Google Scholar

32. H. F. Al-Azzawie and M. S. lhamdani, Hypoglycemic and antioxidant effect of oleuopein in alloxan-diabetic rabbits, Life Sci.78 (2006) 1371–1377; https://doi.org/10.1016/j.lfs.2005.07.0293410.1016/j.lfs.2005.07.029Search in Google Scholar

33. H. Jemai, I. Fki, M. Bouaziz, Z. Bouallaqui, A. El Feki, H. Isoda and S. Sayadi, Lipid-lowering and antioxidant effects of hydroxytyrosol and its triacetylated derivative recovered from olive tree leaves in cholesterol-fed rats, J. Agric. Food Chem.56 (2008) 2630–2636; https://doi.org/10.1021/jf072589s10.1021/jf072589s18380465Search in Google Scholar

34. D. Kotyzova, A. Hodkov and V. Eybl, The effect of olive oil phenolics-hydroxytyrosol and oleuropein on antioxidant defence status in acute arsenic exposed rats, Toxicol. Lett.205 (2011) S222; https://doi.org/10.1016/j.toxlet.2011.05.76110.1016/j.toxlet.2011.05.761Search in Google Scholar