<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Flavonoids in the buds of eight <italic>Populus</italic> species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, <italic>V/V/V</italic>) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from <italic>P. × canadensis</italic> ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and <italic>P. balsamifera</italic> (1.17 and 2.24 g per 100 g, resp.).</p></abstract>

Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. × canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.).

TLC determination of some flavanones in the buds of different genus <italic>Populus</italic> species and hybrids

Department of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

TLC determination of some flavanones in the buds of different genus  species and hybrids

Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid...

TLC determination of some flavanones in the buds of different genus Populus species and hybrids

Published Online: Apr 26, 2018

Page range: 199 - 210

Accepted: Jan 18, 2018

DOI: https://doi.org/10.2478/acph-2018-0018

Keywords
poplar buds, pinocembrin, pinostrobin, TLC, video-densitometry, densitometry

© 2018 Loretta Pobłocka-Olech et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

TLC determination of some flavanones in the buds of different genus Populus species and hybrids

Published Online: Apr 26, 2018

Page range: 199 - 210

Accepted: Jan 18, 2018

DOI: https://doi.org/10.2478/acph-2018-0018

Keywordspoplar buds, pinocembrin, pinostrobin, TLC, video-densitometry, densitometry

© 2018 Loretta Pobłocka-Olech et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
poplar buds, pinocembrin, pinostrobin, TLC, video-densitometry, densitometry