<abstract xmlns="http://www.w3.org/1999/xhtml">Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 μg of venom kg-1 b.m.) lowered the hemorrhagic activity of the venom alone in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L-1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to the isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HRMS and NMR (1H, 13C, HSQC, HMBC and COSY). A quercetrin concentration of 0.11 μmol L-1 prolonged the plasma coagulation time 2.6 times demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.</abstract>

Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 μg of venom kg-1 b.m.) lowered the hemorrhagic activity of the venom alone in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L-1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to the isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HRMS and NMR (1H, 13C, HSQC, HMBC and COSY). A quercetrin concentration of 0.11 μmol L-1 prolonged the plasma coagulation time 2.6 times demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.

Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves

Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET) Facultad de Medicina Universidad de Panamá

Facultad de Ciencias Naturales Exactas y Tecnología Universidad de Panamá

Facultad de Medicina Universidad de Antioquia Medellín, Colombia

Center for Biodiversity and Drug Discovery INDICASAT-AIP, Panama, Panama

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of...

Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves

Published Online: Dec 20, 2014

Page range: 475 - 483

Accepted: Jun 20, 2014

DOI: https://doi.org/10.2478/acph-2014-0033

Keywords
Bothrops asper (Viperidae) venom, Brownea rosademonte (Caesalpinaceae), quercetrin, anticoagulant, antihemorrhagic, antiproteolytic, antihemolytic

© by Sergio Martínez-Luis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves

Published Online: Dec 20, 2014

Page range: 475 - 483

Accepted: Jun 20, 2014

DOI: https://doi.org/10.2478/acph-2014-0033

KeywordsBothrops asper (Viperidae) venom, Brownea rosademonte (Caesalpinaceae), quercetrin, anticoagulant, antihemorrhagic, antiproteolytic, antihemolytic

© by Sergio Martínez-Luis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Bothrops asper (Viperidae) venom, Brownea rosademonte (Caesalpinaceae), quercetrin, anticoagulant, antihemorrhagic, antiproteolytic, antihemolytic