<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam. (Leguminosae). The possible interchangeable usage of the two fruits, however, has long been very controversial. Here, high performance liquid chromatography coupled with diode array detection was developed to explore their chemical fingerprinting profiles. Besides, the amounts of aglycones of saponin compounds, echinocystic acid and oleanolic acid in both fruits were quantified. The results indicated that there was no significant difference in the content of aglycones from the two types of fruits. However, their chromatographic fingerprints showed distinct characteristics. Therefore, the interchangeable application of these fruits has to be taken with a specific precaution.</p></abstract>

Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam. (Leguminosae). The possible interchangeable usage of the two fruits, however, has long been very controversial. Here, high performance liquid chromatography coupled with diode array detection was developed to explore their chemical fingerprinting profiles. Besides, the amounts of aglycones of saponin compounds, echinocystic acid and oleanolic acid in both fruits were quantified. The results indicated that there was no significant difference in the content of aglycones from the two types of fruits. However, their chromatographic fingerprints showed distinct characteristics. Therefore, the interchangeable application of these fruits has to be taken with a specific precaution.

Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD

Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China

School of Health and Pharmacy Guandong Lingnan Institute of Technology, Guangzhou, China

School of Chinese Materia Medica Guangzhou University of Chinese Medicine, Guangzhou, China

Acta Pharmaceutica

Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam....

Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD

Published Online: Dec 31, 2013

Page range: 505 - 515

DOI: https://doi.org/10.2478/acph-2013-0036

Keywords
Gleditsiae Fructus Abnormalis, Gleditsiae Sinensis Fructus, HPLC-DAD, fingerprinting

This content is open access.

Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD

Published Online: Dec 31, 2013

Page range: 505 - 515

DOI: https://doi.org/10.2478/acph-2013-0036

KeywordsGleditsiae Fructus Abnormalis, Gleditsiae Sinensis Fructus, HPLC-DAD, fingerprinting

This content is open access.

Keywords
Gleditsiae Fructus Abnormalis, Gleditsiae Sinensis Fructus, HPLC-DAD, fingerprinting