<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an industrial process was investigated. MIP was prepared using 4-vinylpyridine as monomer, ethylenedimethacrylate as cross linker, 2,2’-azobis-2-methylpropionitrile as initiator and dimethyl formamide as porogen. Use of acetonitrile as a rebinding solvent allows good recognition of the glibenclamide template. It was found that this polymer can be used for determination of trace levels of glibenclamide with a recovery percentage that could reach 87.1 %. Furthermore, the synthesized MIP showed higher selectivity towards glibenclamide than other compounds such as glimepiride and metformine. The synthesized MIP enabled direct determination of the target contaminant after an enrichment step that allowed quantification of glibenclamide at a concentration as low as 0.016 mg L<sup>-1</sup>. Combination of high performance liquid chromatography with MIP-SPE could be successfully used for quality control of pharmaceuticals during the cleaning process in the production of dry drug forms.</p></abstract>

The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an industrial process was investigated. MIP was prepared using 4-vinylpyridine as monomer, ethylenedimethacrylate as cross linker, 2,2’-azobis-2-methylpropionitrile as initiator and dimethyl formamide as porogen. Use of acetonitrile as a rebinding solvent allows good recognition of the glibenclamide template. It was found that this polymer can be used for determination of trace levels of glibenclamide with a recovery percentage that could reach 87.1 %. Furthermore, the synthesized MIP showed higher selectivity towards glibenclamide than other compounds such as glimepiride and metformine. The synthesized MIP enabled direct determination of the target contaminant after an enrichment step that allowed quantification of glibenclamide at a concentration as low as 0.016 mg L-1. Combination of high performance liquid chromatography with MIP-SPE could be successfully used for quality control of pharmaceuticals during the cleaning process in the production of dry drug forms.

Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues

Laboratoire de Chimie Analytique et d’Electrochimie, Université de Carthage Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord B.P.N°676, 1080 Tunis Cedex, Tunisia

Laboratoire National de Contrôle des Médicaments, 1006 Tunis, Tunisia

Acta Pharmaceutica

The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an...

Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues

Published Online: Jul 10, 2013

Page range: 265 - 275

DOI: https://doi.org/10.2478/acph-2013-0017

Keywords
glibenclamide, molecular imprinted polymer, solid phase extraction, HPLC

This content is open access.

Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues

Published Online: Jul 10, 2013

Page range: 265 - 275

DOI: https://doi.org/10.2478/acph-2013-0017

Keywordsglibenclamide, molecular imprinted polymer, solid phase extraction, HPLC

This content is open access.

Keywords
glibenclamide, molecular imprinted polymer, solid phase extraction, HPLC