<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Azolla pinnata</italic> is an aquatic plant that comes from the order of <italic>Pteridophyta</italic> and family of <italic>Azollaceae</italic> that can be found floating on the surface of waters and can only grow at non-moving or slow-moving water areas. This research aims to understand the effect of different extraction methods and fertilizers on <italic>Azolla pinnata</italic> and its secondary metabolites. The fertilizers treatments that were tested were chicken manure and AB. The different extraction methods that were tested were SFE and Soxhlet extraction. In the test, the amount of dry weight of <italic>A.pinnata</italic> obtained from using AB fertilizer was higher compared to chicken manure. For the extraction, both SFE and Soxhlet extraction produced an almost similar amount of extracts but different amounts of secondary metabolites. The amounts of the secondary metabolites were determined and analyzed using GC-MS. The metabolites identified were theamine, bioxirane, acetic acid, 9 methyl-1-decene, glycerine, dihydroxyacetone, thymine, pyranone, 6-fluoro-2-trifluoromethylbenzoic acid, 2-formyl-4,6 dichlorophenyl ester, and isoglutamine. Based on the result, treatment with AB fertilizer provides higher amounts of secondary metabolites in A.pinnata compared to chicken manure, while for extraction methods, SFE provides higher amounts of secondary metabolites in A.pinnata compared to Soxhlet extraction.</p></abstract>

Azolla pinnata is an aquatic plant that comes from the order of Pteridophyta and family of Azollaceae that can be found floating on the surface of waters and can only grow at non-moving or slow-moving water areas. This research aims to understand the effect of different extraction methods and fertilizers on Azolla pinnata and its secondary metabolites. The fertilizers treatments that were tested were chicken manure and AB. The different extraction methods that were tested were SFE and Soxhlet extraction. In the test, the amount of dry weight of A.pinnata obtained from using AB fertilizer was higher compared to chicken manure. For the extraction, both SFE and Soxhlet extraction produced an almost similar amount of extracts but different amounts of secondary metabolites. The amounts of the secondary metabolites were determined and analyzed using GC-MS. The metabolites identified were theamine, bioxirane, acetic acid, 9 methyl-1-decene, glycerine, dihydroxyacetone, thymine, pyranone, 6-fluoro-2-trifluoromethylbenzoic acid, 2-formyl-4,6 dichlorophenyl ester, and isoglutamine. Based on the result, treatment with AB fertilizer provides higher amounts of secondary metabolites in A.pinnata compared to chicken manure, while for extraction methods, SFE provides higher amounts of secondary metabolites in A.pinnata compared to Soxhlet extraction.

The Effect of Different Fertilizer and Extraction Method on Secondary Metabolites of <italic>Azolla Pinnata</italic>

Department of Plant Science, Kulliyyah of Science

International Islamic University Malaysia

Department of Biotechnology, Kulliyyah of Science

Acta Chemica Malaysia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Effect of Different Fertilizer and Extraction Method on Secondary Metabolites of 

Azolla pinnata is an aquatic plant that comes from the order of Pteridophyta and family of Azollaceae that can be found floating on the surface of waters and...

The Effect of Different Fertilizer and Extraction Method on Secondary Metabolites of Azolla Pinnata

Published Online: Jun 29, 2020

Page range: 28 - 32

DOI: https://doi.org/10.2478/acmy-2020-0005

Keywords
Pteridophyta, Azolla pinnata, fertilizers treatments, theamine, bioxirane

© 2020 Muhammad Ameerul Haqim Roshidi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Effect of Different Fertilizer and Extraction Method on Secondary Metabolites of Azolla Pinnata

Published Online: Jun 29, 2020

Page range: 28 - 32

DOI: https://doi.org/10.2478/acmy-2020-0005

KeywordsPteridophyta, Azolla pinnata, fertilizers treatments, theamine, bioxirane

© 2020 Muhammad Ameerul Haqim Roshidi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Pteridophyta, Azolla pinnata, fertilizers treatments, theamine, bioxirane