<abstract xmlns="http://www.w3.org/1999/xhtml">The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day <italic>in vitro</italic> culture. The analysis was conducted using the RT-qPCR technique.Changes in the expression of CD105 and FSP1 could be observed during the 7-day <italic>in vitro</italic> culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.<bold>Running title</bold>: Expression of TGFB1, CD105 and FSP1 in granulosa cells</abstract>

The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.These sub-populations include mural granulosa cells,...

GENE	GENE ACCESSION NUMBER	PRIMER SEQUENCE (5’-3’)	PRODUCT SIZE (BP)
TGFB1	NM_000660	CCTTGCGGAAGTCAATGTACA GGCCTTTCCTGCTTCTCATG	150
CD(ENG) 105	BC014271	CACTAGCCAGGTCTCGAAGG CTGAGGACCAGAAGCACCTC	165
(SFSP1001 A4)	CR450345	TCTTGGGGAAAAGGACAGATGA CCCAACCACATCAGAGGAGT	195
ACTB	NM_001101	CTCAGGAGGAGCAATGATCTTG AAAGACCTGTACGCCAACAC	132

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Published Online: Dec 31, 2020

Page range: 152 - 157

Received: Oct 06, 2020

Accepted: Nov 17, 2020

DOI: https://doi.org/10.2478/acb-2020-0019

Keywords
granulosa cells, TGFB1, CD105, FSP1

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Oligonucleotide sequences of primers used for RT-qPCR analysis

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Published Online: Dec 31, 2020

Page range: 152 - 157

Received: Oct 06, 2020

Accepted: Nov 17, 2020

DOI: https://doi.org/10.2478/acb-2020-0019

Keywordsgranulosa cells, TGFB1, CD105, FSP1

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Oligonucleotide sequences of primers used for RT-qPCR analysis

Keywords
granulosa cells, TGFB1, CD105, FSP1