<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Granulosa cells (GCs) provide the microenvironment necessary for the development of the follicle and the maturation of the oocyte. GCs are associated with reproductive system function and the maintenance of pregnancy by participating in the synthesis of steroid hormones. Many authors point to new ways of using GCs in regenerative medicine and indicate the significant plasticity of this cell population, suggesting that GCs can undergo a transdifferentiation process. Employing primary <italic>in vitro</italic> cell cultures and high-throughput transcriptome analysis via Affymetrix microarrays, this study describes groups of genes associated with enzymatic reactions. 52 genes were identified belonging to four gene ontology biological process terms (GO BP): “coenzyme biosynthetic process”, “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process”. All identified genes showed reduction in the level of mRNA expression during long-term <italic>in vitro</italic> cultivation. Significanthe transcriptomic profile variability was exhibited for the genes (<italic>ELOVL5, ELOVL6</italic> and <italic>GPAM</italic>) involved in enzymatic regulation of fatty acid metabolism.</p><p><bold>Running title</bold>: Enzymatic regulation in granulosa cells</p></abstract>

Granulosa cells (GCs) provide the microenvironment necessary for the development of the follicle and the maturation of the oocyte. GCs are associated with reproductive system function and the maintenance of pregnancy by participating in the synthesis of steroid hormones. Many authors point to new ways of using GCs in regenerative medicine and indicate the significant plasticity of this cell population, suggesting that GCs can undergo a transdifferentiation process. Employing primary in vitro cell cultures and high-throughput transcriptome analysis via Affymetrix microarrays, this study describes groups of genes associated with enzymatic reactions. 52 genes were identified belonging to four gene ontology biological process terms (GO BP): “coenzyme biosynthetic process”, “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process”. All identified genes showed reduction in the level of mRNA expression during long-term in vitro cultivation. Significanthe transcriptomic profile variability was exhibited for the genes (ELOVL5, ELOVL6 and GPAM) involved in enzymatic regulation of fatty acid metabolism.Running title: Enzymatic regulation in granulosa cells

Coenzyme and cofactor metabolism belongs to biochemical processes significantly regulated in human granulosa cells collected after IVF during long-term primary in vitro culture

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Granulosa cells (GCs) provide the microenvironment necessary for the development of the follicle and the maturation of the oocyte. GCs are associated with...

Coenzyme and cofactor metabolism belongs to biochemical processes significantly regulated in human granulosa cells collected after IVF during long-term primary in vitro culture

Published Online: Dec 21, 2019

Page range: 152 - 160

Received: Oct 22, 2019

Accepted: Nov 26, 2019

DOI: https://doi.org/10.2478/acb-2019-0021

Keywords
granulosa cells, culture, coenzyme, cofactor

© 2019 Mariusz J. Nawrocki, Rafał Sibiak, Maciej Brązert, Piotr Celichowski, Leszek Pawelczyk, Błażej Chermuła, Claudia Dompe, Bartosz Kempisty, Paul Mozdziak, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Coenzyme and cofactor metabolism belongs to biochemical processes significantly regulated in human granulosa cells collected after IVF during long-term primary in vitro culture

Published Online: Dec 21, 2019

Page range: 152 - 160

Received: Oct 22, 2019

Accepted: Nov 26, 2019

DOI: https://doi.org/10.2478/acb-2019-0021

Keywordsgranulosa cells, culture, coenzyme, cofactor

© 2019 Mariusz J. Nawrocki, Rafał Sibiak, Maciej Brązert, Piotr Celichowski, Leszek Pawelczyk, Błażej Chermuła, Claudia Dompe, Bartosz Kempisty, Paul Mozdziak, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
granulosa cells, culture, coenzyme, cofactor