<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Reproductive period in woman is characterized by cyclic changes of endometrium and its regeneration. The factors important for proper function of the endometrium are anatomical structure, hormonal control and signalling pathways on molecular-genetic level. New knowledge on uterine microbiome, mutual epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) of endometrial cells refine our conception of changes on cellular level, endometrial receptivity and possible causes of endometrial dysfunction. Substantial information on bacterial colonisation of endometrium were discovered by new diagnostic methods using analysis of ribosomal RNA in 16S subunit which are able to detect and exactly identify bacteria that are not detectable by classic cultivation methods. Endometrial microbiome prevents development of pathogenous microorganisms and modulates function of endometrial cells. It has been proven that stromal cells contribute to regeneration of not only the endometrial stroma, but also of the epithelium. Activity of ovarian steroids and other factors leads to EMT/MET, which ensures different functions of endometrium throughout the menstrual cycle and pregnancy. The endometrial microbiome and mutual transition of stromal and epithelial endometrial cells are necessary for physiological functions of uterine mucosa including implantation and development of an embryo.</p><p><bold>Running title</bold>: Microbiome and cells transition in endometrial function</p></abstract>

Reproductive period in woman is characterized by cyclic changes of endometrium and its regeneration. The factors important for proper function of the endometrium are anatomical structure, hormonal control and signalling pathways on molecular-genetic level. New knowledge on uterine microbiome, mutual epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) of endometrial cells refine our conception of changes on cellular level, endometrial receptivity and possible causes of endometrial dysfunction. Substantial information on bacterial colonisation of endometrium were discovered by new diagnostic methods using analysis of ribosomal RNA in 16S subunit which are able to detect and exactly identify bacteria that are not detectable by classic cultivation methods. Endometrial microbiome prevents development of pathogenous microorganisms and modulates function of endometrial cells. It has been proven that stromal cells contribute to regeneration of not only the endometrial stroma, but also of the epithelium. Activity of ovarian steroids and other factors leads to EMT/MET, which ensures different functions of endometrium throughout the menstrual cycle and pregnancy. The endometrial microbiome and mutual transition of stromal and epithelial endometrial cells are necessary for physiological functions of uterine mucosa including implantation and development of an embryo.Running title: Microbiome and cells transition in endometrial function

The role of uterine microbiome and epithelial-mesenchymal transition in endometrial function

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Reproductive period in woman is characterized by cyclic changes of endometrium and its regeneration. The factors important for proper function of the...

Acinetobacter	Flavobacterium	Pseudomonas
Atopodium	Gardnerella	Shigella
Barnesiella	Lactobacillus	Sneathia
Bifidobacterium	Megasphaera	Sphingobium
Blautia	Moraxella	Sphingomonasor
Clostridium	Parabacteroides	Staphylococcus
Corynebacterium	Parvimonas	Streptococcus
Enterobacter	Prevotella	Vagococcus

CHARACTERISTIC FEATURES OF MESENCHYMAL CELLS	CHARAKTERISTIC M ARKERS OF MESENCHYMAL CELLS	CHARAKERISTIC FEATURES OF EPITHELIAL CELLS	CHARAKERISTIC MARKERS OF EPITHELIAL CELLS
irregular elongated shape	N-cadherin	cylindric, shape kubic or squamous	E-cadherin
absence of junctions	vimentin	strong adhesions intercellular	cytokeratin 8,9
multipolarity	fibronectin	fixation to basal membrane	B-catenin
ability to move	snail 1,2	apico-basal polarity	claudins
invasivity	colagens		laminin
	smooth muscle actin		desmoplakin

The role of uterine microbiome and epithelial-mesenchymal transition in endometrial function

Published Online: Nov 08, 2019

Page range: 146 - 151

Received: Oct 01, 2019

Accepted: Oct 23, 2019

DOI: https://doi.org/10.2478/acb-2019-0020

Keywords
endometrium, uterine microbiome, epithelial-mesenchymal transition, endometrial regeneration, endometrial receptivity, embryo implantation

© 2019 Karel Crha, Pavel Ventruba, Jana Žáková, Michal Ješeta, Radovan Pilka, Jan Vodička, Igor Crha, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Overview of bacteria in the endometrial microbiome most often detected by sequencing of the characteristic hypervariable regions V1-V9 of the rRNA 16S ribosomal subunit

Characteristic features and cellular markers of the epithelial and mesenchymal cells

The role of uterine microbiome and epithelial-mesenchymal transition in endometrial function

Published Online: Nov 08, 2019

Page range: 146 - 151

Received: Oct 01, 2019

Accepted: Oct 23, 2019

DOI: https://doi.org/10.2478/acb-2019-0020

Keywordsendometrium, uterine microbiome, epithelial-mesenchymal transition, endometrial regeneration, endometrial receptivity, embryo implantation

© 2019 Karel Crha, Pavel Ventruba, Jana Žáková, Michal Ješeta, Radovan Pilka, Jan Vodička, Igor Crha, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Overview of bacteria in the endometrial microbiome most often detected by sequencing of the characteristic hypervariable regions V1-V9 of the rRNA 16S ribosomal subunit

Characteristic features and cellular markers of the epithelial and mesenchymal cells

Keywords
endometrium, uterine microbiome, epithelial-mesenchymal transition, endometrial regeneration, endometrial receptivity, embryo implantation