<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are considered the second major cause of sexually transmitted disease after Chlamydiae worldwide. Although the urethra and the uterine cervix serve as the initial sites for gonococcal infections in men and women, infection of the conjunctiva, pharynx, tendons, joints, as well as rectal mucosa are also reported. Objectives: The objectives of this study were to introduce molecular techniques such as polymerase chain reaction (PCR) to detect N. gonorrhoeae directly from endocervical swabs. In addition, it provides a picture of Neisseria gonorrohea infection among a sample of Palestinian women in West Bank. Methods: Two hundred and thirteen endocervical swabs were collected from sexually active married women with endocervical abnormalities attending healthcare clinics. DNA was extracted directly from the swabs and PCR was performed using specific primers targeting the orf1 region of the genome. Results: The results obtained indicated that the percentage of positive cases of N. gonorrhoeae among the women tested was 1.40%. Conclusion: Implementing guidelines for comprehensive screening of men and women with more sensitive tests may improve detection and management of sexually transmitted infections.</p></abstract>

Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are considered the second major cause of sexually transmitted disease after Chlamydiae worldwide. Although the urethra and the uterine cervix serve as the initial sites for gonococcal infections in men and women, infection of the conjunctiva, pharynx, tendons, joints, as well as rectal mucosa are also reported. Objectives: The objectives of this study were to introduce molecular techniques such as polymerase chain reaction (PCR) to detect N. gonorrhoeae directly from endocervical swabs. In addition, it provides a picture of Neisseria gonorrohea infection among a sample of Palestinian women in West Bank. Methods: Two hundred and thirteen endocervical swabs were collected from sexually active married women with endocervical abnormalities attending healthcare clinics. DNA was extracted directly from the swabs and PCR was performed using specific primers targeting the orf1 region of the genome. Results: The results obtained indicated that the percentage of positive cases of N. gonorrhoeae among the women tested was 1.40%. Conclusion: Implementing guidelines for comprehensive screening of men and women with more sensitive tests may improve detection and management of sexually transmitted infections.

Detection of Neisseria gonorrhoeae in Palestinian women using polymerase chain reaction

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are...

Detection of Neisseria gonorrhoeae in Palestinian women using polymerase chain reaction

Published Online: Apr 13, 2018

Page range: 637 - 640

DOI: https://doi.org/10.2478/abm-2010-0081

Keywords
Endocervix, Neisseria gonnorhoeae, Palestine, PCR, sexually transmitted disease

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Detection of Neisseria gonorrhoeae in Palestinian women using polymerase chain reaction

Published Online: Apr 13, 2018

Page range: 637 - 640

DOI: https://doi.org/10.2478/abm-2010-0081

KeywordsEndocervix, Neisseria gonnorhoeae, Palestine, PCR, sexually transmitted disease

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Endocervix, Neisseria gonnorhoeae, Palestine, PCR, sexually transmitted disease