Co-expression of human malaria parasite Plasmodium falciparum orotate phosphoribosyltransferase and orotidine 5’-monophosphate decarboxylase as enzyme complex in Escherichia coli: a novel strategy for drug development

1. Ridley RG. Medical need, scientific opportunity and the drive for antimalarial drugs. Nature. 2002; 415: 686-93.10.1038/415686aSearch in Google Scholar

2. Breman JG, Alilio MS, Mills A. Conquering the intolerable burden of malaria: what’s new, what’s needed: a summary. Am J Trop Med Hyg. 2004; 71(2 Suppl):1-15.10.4269/ajtmh.2004.71.2_suppl.0700001Search in Google Scholar

3. Ndungu FM, Urban BC, Marsh K, Langhorne J. Regulation of immune response by Plasmodiuminfected red blood cells. Parasite Immunol. 2005; 27: 373-84.10.1111/j.1365-3024.2005.00771.xOpen DOISearch in Google Scholar

4. Riley EM. Is T-cell priming required for initiation of pathology in malaria infections? Immunol Today. 1999; 20:228-33.10.1016/S0167-5699(99)01456-5Open DOISearch in Google Scholar

5. Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, et al. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009; 361:455-67.10.1056/NEJMoa0808859Search in Google Scholar

6. Htut ZW. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009; 361:1807-8.10.1056/NEJMc091737Search in Google Scholar

7. Stein WD, Sanchez CP, Lanzer M. Virulence and drug resistance in malaria parasites. Trends Parasitol. 2009; 25:441-3.10.1016/j.pt.2009.07.003Open DOISearch in Google Scholar

8. Krungkrai J, Krungkrai S. Malaria parasite: Genomics, biochemistry and drug target for antimalarial development. Chula Med J. 2006;50:127-42.Search in Google Scholar

9. Kanchanaphum P, Krungkrai J. Kinetic benefits and thermal stability of orotate phosphoribosyltransferase and orotidine 5'-monophosphate decarboxylase enzyme complex in human malaria parasite Plasmodium falciparum. Biochem Biophys Res Commun. 2009; 390: 337-41.10.1016/j.bbrc.2009.09.128Search in Google Scholar

10. Umezu K, Amaya T, Yoshimoto A, Tomita K. Purification and properties of orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarboxylase from baker’s yeast. J Biochem. 1971; 70: 249-62.10.1093/oxfordjournals.jbchem.a129637Search in Google Scholar

11. Quinn CL, Stephenson BT, Switzer RL. Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon. J Biol Chem. 1991; 266:9113-27.10.1016/S0021-9258(18)31559-XSearch in Google Scholar

12. Jones ME. Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis. Annu Rev Biochem. 1980; 49:253-79.10.1146/annurev.bi.49.070180.001345Search in Google Scholar

13. Livingstone LR, Jones ME. The purification and preliminary characterization of UMP synthase from human placenta. J Biol Chem. 1987; 262:15726-33.10.1016/S0021-9258(18)47788-5Search in Google Scholar

14. Yablonski MJ, Pasek DA, Han BD, Jones ME, Traut TW. Intrinsic activity and stability of bifunctional human UMP synthase and its two separate catalytic domains, orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase. J Biol Chem. 1996; 271:10704-8.10.1074/jbc.271.18.10704Search in Google Scholar

15. Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature. 2002; 419:498-511.10.1038/nature01097Search in Google Scholar

16. Krungkrai J, Prapunwattana P, Wichitkul C, Reungprapavut S, Krungkrai SR, Horii T. Molecular biology and biochemistry of malarial parasite pyrimidine biosynthetic pathway. Southeast Asian J Trop Med Public Health. 2003; 34 (Suppl 2):32-44.Search in Google Scholar

17. Krungkrai SR, DelFraino BJ, Smiley JA, Prapunwattana P, Mitamura T, Horii T, et al. A novel enzyme complex of orotate phosphoribosyltransferase and orotidine 5'-monophosphate decarboxylase in human malaria parasite Plasmodium falciparum:physical association, kinetics, and inhibition characterization. Biochemistry. 2005; 44:1643-52.10.1021/bi048439hSearch in Google Scholar

18. Krungkrai SR, Aoki S, Palacpac NM, Sato D, Mitamura T, Krungkrai J, et al. Human malaria parasite orotate phosphoribosyltransferase: functional expression, characterization of kinetic reaction mechanism and inhibition profile. Mol Biochem Parasitol. 2004; 134: 245-55.10.1016/j.molbiopara.2003.12.006Search in Google Scholar

19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54.10.1016/0003-2697(76)90527-3Search in Google Scholar

20. Krungkrai SR, Prapunwattana P, Horii T, Krungkrai J. Orotate phosphoribosyltransferase and orotidine 5'- monophosphate decarboxylase exist as multienzyme complex in human malaria parasite Plasmodium falciparum. Biochem Biophys Res Commun. 2004; 318: 1012-8.10.1016/j.bbrc.2004.04.124Search in Google Scholar

21. Krungkrai J, Wutipraditkul N, Prapunwattana P, Krungkrai SR, Rochanakij S. A nonradioactive highperformance liquid chromatographic microassay for uridine 5'-monophosphate synthase, orotate phosphoribosyltransferase, and orotidine 5'- monophosphate decarboxylase. Anal Biochem. 2001; 299:162-8.10.1006/abio.2001.5431Search in Google Scholar

22. Cleland WW. Statistical analysis of enzyme kinetic data. Methods Enzymol. 1979;63:103-38.10.1016/0076-6879(79)63008-2Search in Google Scholar

23. Floyd EE, Jones ME. Isolation and characterization of the orotidine 5'-monophosphate decarboxylase domain of the multifunctional protein uridine 5'-monophosphate synthase. J Biol Chem. 1985; 260: 9443-51.10.1016/S0021-9258(17)39386-9Search in Google Scholar

24. Tokuoka K, Kusakari Y, Krungkrai SRM, Matsumura H, Kai Y, Krungkrai J, et al. Structural basis for the decarboxylation of orotidine 5'-monophosphate (OMP) by Plasmodium falciparum OMP decarboxylase. J Biochem. 2008; 143:69-78.10.1093/jb/mvm19317981823Search in Google Scholar

25. Fletcher S, Hamilton AD. Targeting protein-protein interactions by rational design: mimicry of protein surfaces. J R Soc Interface. 2006; 3:215-33.10.1098/rsif.2006.0115157874416849232Open DOISearch in Google Scholar

26. Wells JA, McClendon CL. Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature. 2007; 450:1001-9.10.1038/nature0652618075579Search in Google Scholar