<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to validate a new method for determining benzodiazepines in urine via their benzophenone derivatives, based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). Selected benzodiazepines were analysed after acid hydrolysis of urine and extraction by ethyl acetate in the presence of an internal standard. Samples were analysed using electrospray ionization LC-MS/MS in a multiple reaction monitoring mode. The chromatographic run time on a reversed phase C<sub>18</sub> analytical column was set for 9 min. This method was validated in 21 patients receiving methadone. Benzodiazepines intake was established in two out of three patients. LC-MS/MS results were also compared with the rapid immunoassay and the methods showed good agreement. However, in three cases benzodiazepines were detected by LC-MS/MS, but not by the immunoassay. The sensitivity of the developed LC-MS/MS method is comparable to or even higher than of previously reported methods, which makes it suitable as a confirmatory method.</p></abstract>

The aim of this study was to validate a new method for determining benzodiazepines in urine via their benzophenone derivatives, based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). Selected benzodiazepines were analysed after acid hydrolysis of urine and extraction by ethyl acetate in the presence of an internal standard. Samples were analysed using electrospray ionization LC-MS/MS in a multiple reaction monitoring mode. The chromatographic run time on a reversed phase C18 analytical column was set for 9 min. This method was validated in 21 patients receiving methadone. Benzodiazepines intake was established in two out of three patients. LC-MS/MS results were also compared with the rapid immunoassay and the methods showed good agreement. However, in three cases benzodiazepines were detected by LC-MS/MS, but not by the immunoassay. The sensitivity of the developed LC-MS/MS method is comparable to or even higher than of previously reported methods, which makes it suitable as a confirmatory method.

Determination of Benzodiazepines in Urine Via Benzophenone Derivatives Using Liquid Chromatography-Tandem Mass Spectrometry

Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia

Archives of Industrial Hygiene and Toxicology

The aim of this study was to validate a new method for determining benzodiazepines in urine via their benzophenone derivatives, based on liquid...

Determination of Benzodiazepines in Urine Via Benzophenone Derivatives Using Liquid Chromatography-Tandem Mass Spectrometry

Published Online: Dec 23, 2010

Page range: 381 - 388

DOI: https://doi.org/10.2478/10004-1254-61-2010-2058

Keywords
diazepam, lorazepam, presnovki benzodiazepinov, metadonska terapija, zloraba zdravil

This content is open access.

Determination of Benzodiazepines in Urine Via Benzophenone Derivatives Using Liquid Chromatography-Tandem Mass Spectrometry

Published Online: Dec 23, 2010

Page range: 381 - 388

DOI: https://doi.org/10.2478/10004-1254-61-2010-2058

Keywordsdiazepam, lorazepam, presnovki benzodiazepinov, metadonska terapija, zloraba zdravil

This content is open access.

Keywords
diazepam, lorazepam, presnovki benzodiazepinov, metadonska terapija, zloraba zdravil