Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

National Institute for Occupational Safety and Health (NIOSH). NIOSH Alert. Preventing occupational exposures to antineoplastic and other hazardous drugs in health care settings. Publication Number 2004-165. Cincinatti (OH): NIOSH Publication Dissemination; 2004.Search in Google Scholar

WHO Collaborating Centre for Drug Statistics Methodology. Guidelines for ATC classification and DDD assignment 2010 [pristup 17. siječnja 2010.]. Dostupno na http://www.whocc.no/filearchive/publications/2010guidelines.pdfSearch in Google Scholar

Vrhovac B, urednik. Farmakoterapijski priručnik. 4. izd. Zagreb: Medicinska naklada; 2003.Search in Google Scholar

International Agency for Research on Cancer (IARC). Complete List of Agents evaluated and their classification [pristup 17. siječnja 2010.]. Dostupno na http://monographs.iarc.fr/ENG/Classification/index.phpSearch in Google Scholar

International Agency for Research on Cancer (IARC). Agents reviewed by the IARC Monographs Volume 1-100A (by CAS numbers) [pristup 17. siječnja 2010.]. Dostupno na http://monographs.iarc.fr/ENG/Classification/ListagentsCASnos.pdfSearch in Google Scholar

International Agency for Research on Cancer (IARC). Summaries and Evaluations: Melphalan, Medphalan and Merphalan [pristup 17. siječnja 2010.]. Dostupno na http://www.inchem.org/documents/iarc/vol09/melphalan.htmlSearch in Google Scholar

Falck K, Gröhn P, Sorsa M. Mutagenicity in urine of nurses handling cytostatic drugs. Lancet 1979;1:1250-1.10.1016/S0140-6736(79)91939-1Search in Google Scholar

Sanderson BJ, Shield AJ. Mutagenic damage to mammalian cells by therapeutic alkylating agents. Mutat Res 1996;355:41-57.10.1016/0027-5107(96)00021-8Search in Google Scholar

Sanderson BJ, Ferguson LR, Denny WA. Mutagenic and carcinogenic properties of platinum-based anticencer drugs. Mutat Res 1996;355:59-70.10.1016/0027-5107(96)00022-XSearch in Google Scholar

Jacobson MA, Stack HF, Waters MD. Genetic activity profiles of anticancer drugs. Mutat Res 1996;355:171-208.10.1016/0027-5107(96)00028-0Search in Google Scholar

Singh B, Gupta RS. Mutagenic responses of thirteen anticancer drugs on mutation induction at multiple genetic loci and sister chromatid exchanges in chinese hamster ovary cells. Cancer Res 1983;43:577-84.Search in Google Scholar

Page C, Curtis M, Sutter M, Walker M, Hoffman B, urednici. Integrated Pharmacology. 2. izd. Edinburgh: Mosby International Ltd.; 2002.Search in Google Scholar

Rang HP, Dale MM, Riffer JM, Moore PK, urednici. Pharmacology. 5. izd. Edinburgh: Churchill Livingstone; 2003.Search in Google Scholar

Henke Yarbro C, Hansen Frogge M, Goodman M, urednici. Cancer Nursing: Principles and Practice. 6. izd. Sudbury: Jones and Bartlett Publishers Inc.; 2005.Search in Google Scholar

Drabløs F, Feyzi E, Aas PA, Vaagbø CB, Kavli B, Bratlie MS, Peña-Diaz J, Otterlei M, Slupphaug G, Krokan HE. Alkylation damage in DNA and RNA-repair mechanisms and medical significance. DNA Repair 2004;3:1389-407.10.1016/j.dnarep.2004.05.004Search in Google Scholar

Chen C-S, Lin JT, Goss KA, He Y, Halpert JR, Waxman DJ. Activation of the anticancer prodrugs cyclophosphamide and ifosfamide: identification of cytochrome P450 2B enzymes and site-specific mutants with improved enzyme kinetics. Mol Pharmacol 2004;65:1278-85.10.1124/mol.65.5.1278Search in Google Scholar

Chabner B, Longo DL, urednici. Cancer Chemotherapy and Biotherapy: Principles and Practice. 4. izd. Philadelphia (PA): Lippincot Williams and Wilkins; 2006.Search in Google Scholar

Skeel RT, urednik. Handbook of Cancer Chemotherapy. 7. izd. Philadelphia: Lippincot Williams and Wilkins; 2007.Search in Google Scholar

Paci A, Rieutord A, Brion F, Prognon P. Separation methods for alkylating antineoplastic compounds. J Chrom B 2001;764:255-87.10.1016/S0378-4347(01)00280-8Search in Google Scholar

British Columbia Cancer Agency (BCCA). BC Cancer Agency Cancer Drug Manual© Gemcitabine [pristup 20. siječnja 2010.]. Dostupno na http://www.bccancer.bc.ca/NR/rdonlyres/61DCBA09-705A-485C-9058-05B892064E80/19547/Gemcitabinemonograph_2Nov06.pdfSearch in Google Scholar

American Cancer Society, Lenhard RE, Osteen RT, Gansler TS, urednici. Clinical Oncology. 2. izd. Malden (MA): Blackwell Science Inc.; 2001.Search in Google Scholar

Schellens JHM, McLeod HL, Newell DR, urednici. Cancer Clinical Pharmacology. New York (NY): Oxford University Press Inc., 2005.Search in Google Scholar

Giaccone G, Schilsky R, Sondel P, urednici. Cancer Chemotherapy and Biological Response Modifiers: Annual 19. Amsterdam: Elsevier Science B.V.; 2001.Search in Google Scholar

Vig BK. Genetic toxicology of mitomycin C, actinomycins, daunomycin and adriamycin. Mutat Res 1977;48:189-238.10.1016/0165-1110(77)90022-7Search in Google Scholar

Gewirtz DA. A critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem Pharmacol 1999;57:727-41.10.1016/S0006-2952(98)00307-4Search in Google Scholar

Vig BK, Lewis R. Genetic toxicology of bleomycin. Mutat Res 1978;55:121-45.10.1016/0165-1110(78)90019-2Search in Google Scholar

Povirk LF, Austin MJF. Genotoxicity of bleomycin. Mutat Res 1991;257:127-43.10.1016/0165-1110(91)90022-NSearch in Google Scholar

Erexson GL, Bryant MF, Kwanyuen P, Kligerman AD. Bleomycin sulfate-induced micronuclei in human, rat, and mouse peripheral blood lymphocytes. Environ Mol Mutagen 1995;25:31-6.10.1002/em.2850250106Search in Google Scholar

González-Cid M, Mudry M, Larripa I. Chromosome damage induced by carboplatin (CBDCA). Toxicol Lett 1995;76:97-103.10.1016/0378-4274(94)03202-ISearch in Google Scholar

British Columbia Cancer Agency (BCCA). BC Cancer Agency Cancer Drug Manual© Oxaliplatin [pristup 20. siječnja 2010.]. Dostupno na http://www.bccancer.bc.ca/NR/rdonlyres/D0132051-8601-4B5E-89A0-5BB3B4739C83/31059/Oxaliplatinmonograph_1Nov08_formatted.pdfSearch in Google Scholar

Barton-Burke M, Wilkes GM, Ingwersen K, urednici. Cancer Chemotherapy: A Nursing Process Approach. 3. izd. Sudbury (MA): Jones and Bartlett Publishers, Inc.; 2001.Search in Google Scholar

British Columbia Cancer Agency (BCCA). BC Cancer Agency Cancer Drug Manual© Procarbazine [pristup 20. siječnja 2010.]. Dostupno na http://www.bccancer.bc.ca/NR/rdonlyres/7B97C0C8-FFF9-4317-8397-81E6268746A9/29592/Procarbazinemonograph_1July08.pdfSearch in Google Scholar

British Columbia Cancer Agency (BCCA). BC Cancer Agency Cancer Drug Manual© Amsacrine [pristup 20. siječnja 2010.]. Dostupno na http://www.bccancer.bc.ca/NR/rdonlyres/DC04BEFB-BF13-4A48-91A8-976A9650C6B8/30156/Amsacrinemonograph_1Sept08.pdfSearch in Google Scholar

Yarbro JW. Mechanism of action of hydroxyurea. Semin Oncol 1992;19(Suppl 9):1-10.Search in Google Scholar

Donehower RC. An overview of the clinical experience with hydroxyurea. Semin Oncol 1992;19(Suppl 9):11-9.Search in Google Scholar

British Columbia Cancer Agency (BCCA). BC Cancer Agency Cancer Drug Manual© Estramustine [pristup 20. siječnja 2010.]. Dostupno na http://www.bccancer.bc.ca/NR/rdonlyres/93C68101-0CC1-43AA-BD89-DEB4E83F62FE/34099/Estramustinemonograph_1May09.pdfSearch in Google Scholar

Migliore L, Cocchi R, Scarpato R, Sbrana I. Induction of aneuploidy by the antineoplastic drug estramustine in human lymphocytes. Mutat Res 1998;412:33-40.10.1016/S1383-5718(97)00165-4Search in Google Scholar

Connor TH, McDiarmid MA. Preventing occupational exposures to antineoplastic drugs in health care settings. CA Cancer J Clin 2006;56:354-65.10.3322/canjclin.56.6.354Search in Google Scholar

Occupational Safety and Health Administration (OSHA). Office of Occupational Medicine Work Practice Guidelines for Personnel Dealing with Cytotoxic (Antineoplastic) Drugs. Occupational Safety and Health Administration Instruction publication. Washington (DC): US Dept of Labor; 1986.Search in Google Scholar

American Society of Health-System Pharmacists. ASHP guidelines on handling hazardous drugs. Am J Health-Syst Pharm 2006;63:1172-93.10.2146/ajhp050529Search in Google Scholar

QuapoS 4: Quality Standard for the Oncology Pharmacy Service With Commentary. 4. izd. Oldenburg: Onkopress; 2009.Search in Google Scholar

Turci R, Sottani C, Spagnoli G, Minoia C. Biological and environmental monitoring of hospital personnel exposed to antineoplastic agent: a review of analytical methods. J Chrom B 2003;789:169-209.10.1016/S1570-0232(03)00100-4Search in Google Scholar

World Health Organization (WHO). Biomarkers In Risk Assessment: Validity And Validation. Environmental Health Criteria 222, 2001 [pristup 20. siječnja 2010.]. Dostupno na http://www.inchem.org/documents/ehc/ehc/ehc222.htmSearch in Google Scholar

Norppa H, Sorsa M, Vainio H, Grohn P, Heinonen E, Holsti L, Nordman E. Increased sister chromatid exchange frequencies in lymphocytes of nurses handling cytostatic drugs. Scand J Work Environ Health 1980;6:299-301.10.5271/sjweh.2605Search in Google Scholar

Waksvik H, Klepp O, Brøgger A. Chromosome analyses of nurses handling cytostatic agents. Cancer Treat Rep 1981;65:607-10.Search in Google Scholar

Kolmodin-Hedman B, Hartvig P, Sorsa M, Falck K. Occupational handling of cytostatic drugs. Arch Toxicol 1983;54:25-33.10.1007/BF00277812Search in Google Scholar

Stiller A, Obe G, Boll I, Pribilla W. No elevation of the frequencies of chromosomal alterations as a consequence of handling cytostatic drugs: Analyses with peripheral blood and urine of hospital personnel. Mutat Res 1983;121:253-9.10.1016/0165-7992(83)90211-7Search in Google Scholar

Barale R, Sozzi G, Toniolo P, Borghi O, Reali D, Loprieno N, Della Porta G. Sister-chromatid exchanges in lymphocytes and mutagenicity in urine of nurses handling cytostatic drugs. Mutat Res 1985;157:235-40.10.1016/0165-1218(85)90121-1Search in Google Scholar

Jordan DK, Patil SR, Jochimsen PR, Lachenbruch PA, Corder MP. Sister chromatid exchange analysis in nurses handling antineoplastic drugs. Cancer Invest 1986;4:101-7.10.3109/07357908609038253Search in Google Scholar

Pohlová H, Černá M, Rössner P. Chromosomal aberrations, SCE and urine mutagenicity in workers occupationally exposed to cytostatic drugs. Mutat Res 1986;174:213-7.10.1016/0165-7992(86)90154-5Search in Google Scholar

Stücker I, Hirsch A, Doloy T, Bastie-Sigeac I, Hémon D. Urine mutagenicity, chromosomal abnormalities and sister chromatid exchanges in lymphocytes of nurses handling cytostatic drugs. Int Arch Occup Environ Health 1986;57:195-205.10.1007/BF00405788Search in Google Scholar

Benhamou S, Pot-Deprun J, Sancho-Garnier H, Chouroulinkov I. Sister-chromatid exchanges and chromosomal aberrations in lymphocytes of nurses handling cytostatic agents. Int J Cancer 1988;41:350-3.10.1002/ijc.2910410305Search in Google Scholar

Sorsa M, Pyy L, Salomaa S, Nyland L, Yager JW. Biological and environmental monitoring of occupational exposure to cyclophosphamide in industry and hospitals. Mutat Res 1988;204:465-79.10.1016/0165-1218(88)90042-0Search in Google Scholar

Krepinsky A, Bryant DW, Davison L, Heddle J, McCalla DR, Douglas G, Michalko K. Comparison of three assays for genetic effects of antineoplastic drugs on cancer patients and their nurses. Environ Mol Mutagen 1990;15:83-92.10.1002/em.2850150205Search in Google Scholar

Oestreicher U, Stephan G, Glatzel M. Chromosome and SCE analysis in peripheral lymphocytes of persons occupationally exposed to cytostatic drugs handled with and without use of safety covers. Mutat Res 1990;242:271-7.10.1016/0165-1218(90)90045-4Search in Google Scholar

Sarto F, Trevisan A, Tomanin R, Canova A, Fiorentino M. Chromosomal aberrations, sister chromatid exchanges and urinary thioethers in nurses handling antineoplastic drugs. Am J Ind Med 1990;18:689-95.10.1002/ajim.47001806072264567Search in Google Scholar

Guinee EP, Beuman GH, Hageman G, Welle IJ, Kleinjans JC. Evaluation of genotoxic risk of handling cytostatic drugs in clinical pharmacy practice. Pharm Weekblad 1991;13:78-82.10.1007/BF019749851870947Search in Google Scholar

Milković-Kraus S, Horvat Đ. Chromosomal abnormalities among nurses occupationally exposed to antineoplastic drugs. Am J Ind Med 1991;19:771-4.10.1002/ajim.4700190609Search in Google Scholar

Thiringer G, Granung G, Holmén A, Hogstedt B, Jarvhom B, Jonsson D, Persson L, Wahlstrom J, Westin J. Comparison of methods for the biomonitoring of nurses handling antitumor drugs. Scand J Work Environ Health 1991;17:133-8.10.5271/sjweh.1724Search in Google Scholar

Şardaş S, Gök, S, Karakaya AE. Sister chromatid exchanges in lymphocytes of nurses handling antineoplastic drugs. Toxicol Lett 1991;55:311-5.10.1016/0378-4274(91)90012-USearch in Google Scholar

Goloni-Bertollo EM, Tajara EH, Manzato AJ, Varella-Garcia M. Sister chromatid exchanges and chromosome aberrations in lymphocytes of nurses handling antineoplastic drugs. Int J Cancer 1992;50:341-4.10.1002/ijc.2910500302Search in Google Scholar

McDiarmid MA, Kolodner K, Humphrey F, Putman D, Jacobson-Kram D. Baseline and phosphoramide mustard-induced sister-chromatid exchanges in pharmacists handling anti-cancer drugs. Mutat Res 1992;279:199-204.10.1016/0165-1218(92)90067-ASearch in Google Scholar

Gorecka D, Gorski T. The influence of cigarette smoking on sister chromatid exchange frequencies in peripheral lymphocytes among nurses handling cytostatic drugs. Pol J Occup Med Environ Health 1993;6:143-8.Search in Google Scholar

Roth S, Norppa H, Järventaus H, Kyyrönen P, Ahonen M, Lehtomäki J, Sainio H, Sorsa M. Analysis of chromosomal aberrations, sister chromatid exchanges and micronuclei in peripheral lymphocytes of pharmacists before and after working with cytostatic drugs. Mutat Res 1994;325:157-62.10.1016/0165-7992(94)90079-5Search in Google Scholar

Peschke M, Nagel S, Haamann F, Melzer S, Meier K. Cytogenetic monitoring of pharmaceutical staff working with cytostatic drug preparations: A 5-year follow-up. J Oncol Pharm Pract 1995;1:33-9.10.1177/107815529500100104Search in Google Scholar

Thulin H, Sundberg E, Hansson K, Cole J, Hartley-Asp B. Occupational exposure to nor-nitrogen mustard: Chemical and biological monitoring. Toxicol Ind Health 1995;11:89-97.10.1177/074823379501100108Search in Google Scholar

Brumen V, Horvat Đ. Work environment influence on cytostatics-induced genotoxicity in oncologic nurses. Am J Ind Med 1996;30:67-71.10.1002/(SICI)1097-0274(199607)30:1<67::AID-AJIM11>3.0.CO;2-6Search in Google Scholar

Ensslin AS, Huber R, Pethran A, Rommelt H, Schierl R, Kulka U, Fruhmann G. Biological monitoring of hospital pharmacy personnel occupationally exposed to cytostatic drugs: urinary excretion and cytogenetic studies. Int Arch Occup Environ Health 1997;70:205-8.10.1007/s004200050208Search in Google Scholar

Fučić A, Jazbec A, Mijić A, Šešo-Šimić Đ, Tomek R. Cytogenetic consequences after occupational exposure to antineoplastic drugs. Mutat Res 1998;416:59-66.10.1016/S1383-5718(98)00084-9Search in Google Scholar

Kevekordes S, Gebel TW, Hellwig M, Dames W, Dunkelberg H. Human effect monitoring in cases of occupational exposure to antineoplastic drugs: A method comparison. Occup Environ Med 1998;55:145-9.10.1136/oem.55.3.145Search in Google Scholar

Mahrous HS, Ismail SR, Hashishe MM, Kohail HM. Sister chromatid exchanges and chromosome aberrations in lymphocytes of medical personnel handling cytostatic drugs. J Egypt Public Health Assoc 1998;73:297-323.Search in Google Scholar

Kašuba V, Rozgaj R, Garaj-Vrhovac V. Analysis of sister chromatid exchange and micronuclei in peripheral blood lymphocytes of murses handling cytostatic drugs. J Appl Toxicol 1999;19:401-4.10.1002/(SICI)1099-1263(199911/12)19:6<401::AID-JAT592>3.0.CO;2-HSearch in Google Scholar

Lanza A, Robustelli della Cuna FS, Zibera C, Pedrazzoli P, Robustelli della Cuna G. Somatic mutations at the T-cell antigen receptor in antineoplastic drug-exposed populations: comparison with sister chromatid exchange frequency. Int Arch Environ Health 1999;72:315-22.10.1007/s004200050381Search in Google Scholar

Pilger A, Kohler I, Stettner H, Mader RM, Rizovski B, Terkola R, Diem E, Franz-Hainzl E, Konnaris C, Valic E, Rudiger HV. Long-term monitoring of sister chromatid exchanges and micronucleus formation in pharmacy personnel occupationally exposed to cytostatic drugs. Int Arch Occup Environ Health 2000;73:442-8.10.1007/s004200000164Search in Google Scholar

Jakab MG, Major J, Tompa A. Follow-up genotoxicological monitoring of nurses handling antineoplastic drugs. J Tox Environ Health 2001;62:307-18.10.1080/152873901300018011Search in Google Scholar

Tompa A, Jakab M, Biro A, Magyar B, Fodor Z, Klupp T, Major J. Chemical Safety and Health Conditions among Hungarian Hospital Nurses. Ann NY Acad Sci 2006;1076:635-48.10.1196/annals.1371.054Search in Google Scholar

Ikeda K, Yagi Y, Tkegami M, Lu Y, Morimoto K, Kurokawa N. Efforts to ensure safety of hospital pharmacy personnel occupationally exposed to antineoplastic drugs during a preparation task. Hosp Pharm 2007;42:209-18.10.1310/hpj4203-209Search in Google Scholar

Kopjar N, Kašuba V, Rozgaj R, Želježić D, Milić M, Ramić S, Pavlica V, Milković-Kraus S. The genotoxic risk in health care workers occupationally exposed to cytotoxic drugs - A comprehensive evaluation by the SCE assay. J Environ Sci Health A 2009;44:462-79.10.1080/10934520902719845Search in Google Scholar

Kopjar N, Garaj-Vrhovac V, Kašuba V, Rozgaj R, Ramić S, Pavlica V, Želježić D. Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs: A multi-biomarker approach. Int J Hyg Environ Health 2009;212:414-31.10.1016/j.ijheh.2008.10.001Search in Google Scholar

Nikula E, Kiviniitty K, Leisti J, Taskinen PJ. Chromosome aberrations in lymphocytes of nurses handling cytostatic agents. Scand J Work Environ Health 1984;10:71-4.10.5271/sjweh.2355Search in Google Scholar

Rössner P, Černá M, Pokorána D, Hájek V, Petr J. Effect of ascorbic acid prophylaxis on the frequency of chromosome aberrations, urine mutagenicity and nucleolus test in workers occupationally exposed to cytostatic drugs. Mutat Res 1988;208:149-53.10.1016/0165-7992(88)90051-6Search in Google Scholar

Medkova J. Cytogenetic analysis of peripheral lymphocytes in occupationally exposed health personnel. Acta Univ Palack Olomuc Facult Med 1990;126:93-106.Search in Google Scholar

Cooke J, Williams J, Morgan RJ, Cooke P, Calvert RT. Use of cytogenetic methods to determine mutagenic changes in the blood of pharmacy personnel and nurses who handle cytotoxic agents. Am J Hosp Pharm 1991;48:1199-205.10.1093/ajhp/48.6.1199Search in Google Scholar

Milković-Kraus S, Kraus O, Kršnjavi H, Kubelka D. Environmental effects on chromosomes in oncology and radiology department personnel. Prev Med 1992;21:498-502.10.1016/0091-7435(92)90056-NSearch in Google Scholar

Grummt T, Grummt H-J, Schott G. Chromosomal aberrations in peripheral lymphocytes of nurses and physicians handling antineoplastic drugs. Mutat Res 1993;302:19-24.10.1016/0165-7992(93)90085-ASearch in Google Scholar

Anwar WA, Salama SI, Serafy MM, Hermida S, Hafez AS. Chromosomal aberrations and micronucleus frequency in nurses occupationally exposed to cytotoxic drugs. Mutagenesis 1994;9:315-7.10.1093/mutage/9.4.315Search in Google Scholar

Sessink PJM, Cerná M, Rössner P, Pastorkova A, Bavarova H, Frankova K, Anzion RB, Bos RP. Urinary cyclophosphamide excretion and chromosomal aberrations in peripheral blood lymphocytes after occupational exposure to antineoplastic agents. Mutat Res 1994;309:193-9.10.1016/0027-5107(94)90092-2Search in Google Scholar

Rubeš J, Kucharová S, Vozdová M, Musilová P, Zudová Z. Cytogenetic analysis of peripheral lymphocytes in medical personnel by means of FISH. Mutat Res 1998;412:293-8.10.1016/S1383-5718(97)00201-5Search in Google Scholar

Ansari-Lari M, Saadat M, Shahryari M, Farhud DD. Sister Chromatid Exchanges and Micronuclei in Lymphocyte of Nurses Handling Antineoplastic Drug. Iranian J Publ Health 2001;30:37-40.Search in Google Scholar

Burgaz S, Karahalil B, Canhi Z, Terzioglu F, Ancel G, Anzion RB, Bos RP, Huttner E. Assessment of genotoxic damage in nurses occupationally exposed to antineoplastics by the analysis of chromosomal aberrations. Hum Exp Toxicol 2002;21:129-35.10.1191/0960327102ht230oa12102538Search in Google Scholar

Xu SJ Wang JX Yang DP. [An investigation on the chromosomal damage in nurses occupationally exposed to antineoplastic drugs, in Chinese]. Chin J Prev Med 2003;37:119-20.Search in Google Scholar

Domínguez Odio A, Batista Duharte A, Carnesoltas D, Romero García LI, Lóriega Loaces E, Cuello Almarales D, Landrove Mora Y, García Cabrera L. Efectos citogenéticos por exposición ocupacional a citostáticos [Cytogenetic effects after occupational exposure to cytostatics, in Spanish]. Rev Med IMSS 2004;42:487-92.Search in Google Scholar

Cavallo D, Ursini CL, Perniconi B, Di Francesco A, Giglio M, Rubino FM, Marinaccio A, Iavicoli S. Evaluation of genotoxic effects induced by exposure to antineoplastic drugs in lymphocytes and exfoliated buccal cells of oncology nurses and pharmacy employees. Mutat Res 2005;587:45-51.10.1016/j.mrgentox.2005.07.00816202645Search in Google Scholar

Testa A, Giachelia M, Palma S, Appolloni M, Padua L, Tranfo G, Spagnoli M, Trindelli D, Cozzi R. Occupational exposure to antineoplastic agents induces a high level of chromosome damage. Lack of an effect of GST polymorphisms. Toxicol Appl Pharmacol 2007;223:46-55.10.1016/j.taap.2007.05.006Search in Google Scholar

Mušák L, Poláková V, Halašová E, Osina O, Vodičková L, Buchancová J, Hudečková H, Vodička P. Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency. Interdisc Toxicol 2009;2:13-7.10.2478/v10102-009-0002-6Search in Google Scholar

Yager JW, Sorsa M, Selvin S. Micronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to alkylating cytostatic drugs. IARC Sci Publ 1988;89:213-6.Search in Google Scholar

Machado-Santelli GM, Cerqueira EM, Oliveira CT, de Braganca Peira CAA. Biomonitoring of nurses handling antineoplastic drugs. Mutat Res 1994;332:203-8.10.1016/0165-1218(94)90007-8Search in Google Scholar

Garaj-Vrhovac V, Kopjar N. Micronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to antineoplastic drugs. Radiol Oncol 1998;32:385-92.Search in Google Scholar

Burgaz S, Karahalil B, Bayrak P, Taşkin L, Yavuzaslan F, Bökesoy I, Anzion RBM, Bos RP, Platin N. Urinary cyclophosphamide excretion and micronuclei frequencies in periperal lymphocytes and in exfoliated buccal epithelial cells of nurses handling antineoplastics. Mutat Res 1999;439:97-104.10.1016/S1383-5718(98)00180-6Search in Google Scholar

Maluf SW, Erdtmann B. Evaluation of occupational genotoxic risk in a Brazilian hospital. Genetics Mol Biol 2000;23:485-8.10.1590/S1415-47572000000200040Search in Google Scholar

Maluf SW, Erdtmann B. Follow-up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesis-block micronuclei analysis and single cell gel electrophoresis assay. Mutat Res 2000;471:21-7.10.1016/S1383-5718(00)00107-8Search in Google Scholar

Hessel H, Radon K, Pethran A, Maisch B, Grobmair S, Sautter I, Fruhmann G. The genotoxic risk of hospital, pharmacy and medical personnel occupationally exposed to cytostatic drugs-evaluation by the micronucleus assay. Mutat Res 2001;497:101-9.10.1016/S1383-5718(01)00236-4Search in Google Scholar

Deng H, Zhang M, He J, Wu W, Jin L, Zheng W, Lou J, Wang B. Investigating genetic damage in workers occupationally exposed to methotrexate using three genetic end-points. Mutagenesis 2005;20:351-7.10.1093/mutage/gei04816037120Search in Google Scholar

Deng H, Lou J, Zhang M, Wu W, Jin L, Chen S, Zheng W, Wang B, He J. Detecting the cytogenetic effects in workers occupationally exposed to vincristine with four genetic tests. Mutat Res 2006;599:152-9.10.1016/j.mrfmmm.2006.02.00316580025Search in Google Scholar

Laffon B, Teixeira JP, Silva S, Loureiro J, Torres J, Pásaro E, Méndez J, Mayan O. Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes. Am J Ind Med 2006;48:128-36.10.1002/ajim.2018916032741Search in Google Scholar

Cavallo D, Ursini CL, Omodeo-Sale E, Iavicoli S. Micronucleus induction and FISH analysis in buccal cells and lymphocytes of nurses handling antineoplastic drugs. Mutat Res 2007;628:11-8.10.1016/j.mrgentox.2006.10.014Search in Google Scholar

Rekhadevi PV, Sailaja N, Chandrasekhar M, Mahboob M, Rahman MF, Grover P. Genotoxicity assessment in oncology nurses handling anti-neoplastic drugs. Mutagenesis 2007;22:395-401.10.1093/mutage/gem032Search in Google Scholar

Cornetta T, Padua L, Testa A, Ievoli E, Festa F, Tranfo G, Baccelliere L, Cozzi R. Molecular biomonitoring of a population of nurses handling antineoplastic drugs. Mutat Res 2008;638:75-82.10.1016/j.mrfmmm.2007.08.017Search in Google Scholar

Rombaldi F, Cassini C, Salvador M, Saffi J, Erdtmann B. Occupational risk assessment of genotoxicity and oxidative stress in workers handling anti-neoplastic drugs during a working week. Mutagenesis 2009;24:143-8.10.1093/mutage/gen060Search in Google Scholar

Ündeger Ü, Basaran N, Kars A, Guc D. Assessment of DNA damage in nurses handling antineoplastic drugs by the alkaline COMET assay. Mutat Res 1999;439:277-85.10.1016/S1383-5718(99)00002-9Search in Google Scholar

Kopjar N, Garaj-Vrhovac V. Application of the alkaline comet assay in human biomonitoring for genotoxicity: A study on Croatian medical personnel handling antineoplastic drugs. Mutagenesis 2001;16:71-8.10.1093/mutage/16.1.7111139601Search in Google Scholar

Yang D, Xu S, Wang J. [The study of DNA damage of peripheral lymphocytes in the nurses occupationally exposed to anticancer drugs, in Chinese]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2002;20:197-9.Search in Google Scholar

Ursini CL, Cavallo D, Colombi A, Giglio M, Marinaccio A, Iavicoli S. Evaluation of early DNA damage in healthcare workers handling antineoplastic drugs. Int Arch Occup Environ Health 2006;80:134-40.10.1007/s00420-006-0111-x16761155Search in Google Scholar

Yoshida J, Kosaka H, Tomioka K, Kumagai S. Genotoxic risks to nurses from contamination of the work environment with antineoplastic drugs in Japan. J Occup Health 2006;48:517-22.10.1539/joh.48.51717179646Search in Google Scholar

Sasaki M, Dakeishi M, Hoshi S, Ishii N, Murata K. Assessment of DNA damage in Japanese nurses handling antineoplastic drugs by the comet assay. J Occup Health 2008;50:7-12.10.1539/joh.50.718285639Search in Google Scholar

Izdes S, Sardas S, Kadioglu E, Kaymak C, Ozcagli E. Assessment of Genotoxic damage in nurses occupationally exposed to anaesthetic gases or antineoplastic drugs by the comet assay. J Occup Health 2009;51:283-6.10.1539/joh.M8012Search in Google Scholar

Perry P, Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature (London) 1974;251:156-8.10.1038/251156a0Search in Google Scholar

Latt S, Allen J, Bloom SE, Carrano A, Falke E, Kram D, Schneider E, Schreck R, Tice R, Whitfield B, Wollf S. Sister chromatid exchanges: A report of the Gene-Tox Program. Mutat Res 1981;87:17-62.10.1016/0165-1110(81)90003-8Search in Google Scholar

Carrano AV, Natarajan AT. Considerations for population monitoring using cytogenetic techniques. Mutat Res 1988;204:379-406.10.1016/0165-1218(88)90036-5Search in Google Scholar

Morimoto K. Induction of sister chromatid exchanges and cell division delays in human lymphocytes by microsomal activation of benzene. Cancer Res 1983;43:1330-4.Search in Google Scholar

Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DEG, Tice R, Waters MD, Aitio A. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat Res 2000;463:111-72.10.1016/S1383-5742(00)00049-1Search in Google Scholar

Carrano AV, Moore DH. The rationale and methodology for quantifying sister chromatid exchange in humans. U: Heddle JA, urednik. Mutagenicity: New Horizons in Genetic Toxicology. New York (NY): Academic Press; 1982. str. 267-304.10.1016/B978-0-12-336180-6.50014-4Search in Google Scholar

Bonassi S, Fontana V, Ceppi M, Barale R, Bigeri A. Analysis of correlated data in human biomonitoring studies. The case of high sister chromatid exchange frequency cells. Mutat Res 1999;438:13-21.10.1016/S1383-5718(98)00153-3Search in Google Scholar

Ponzanelli I, Landi S, Bernacchi F, Barale R. The nature of high frequency sister chromatid exchange cells (HFCs). Mutagenesis 1997;12:329-33.10.1093/mutage/12.5.329Search in Google Scholar

Hirsch BA, Sentz KK, McGue M. Genetic and environmental influences on baseline SCE. Environ Mol Mutagen 1992;20:2-11.10.1002/em.2850200103Search in Google Scholar

Lazutka JR, Dedonyte V, Krapavickaite D. Sister-chromatid exchanges and their distribution in human lymphocytes in relation to age, sex and smoking. Mutat Res 1994;306:173-80.10.1016/0027-5107(94)90028-0Search in Google Scholar

Bolognesi C, Abbondandolo A, Barale R, Casalone R, Dalprà L, De Ferrari M, Degrassi F, Forni A, Lamberti L, Lando C, Migliore L, Padovani P, Pasquini R, Puntoni R, Sbrana I, Stella M, Bonassi S. Age-ralated increase of baseline frequencies of sister chromatid exchanges, chromosome aberrations and micronuclei in human lymphocytes. Cancer Epidem Biom Prev 1997;6:249-56.Search in Google Scholar

Bender MA, Preston J, Leonard RC, Pyatt BE, Gooch PC, Shelby MD. Chromosomal aberration and sister chromatid exchange frequencies in peripheral lymphocytes of a large human population sample. Mutat Res 1988;204:421-33.10.1016/0165-1218(88)90038-9Search in Google Scholar

Anderson D, Francis AJ, Godbert P, Jenkinson PC, Butterworth KR. Variability in chromosome aberrations, sister chromatid exchanges, and mitogen-induced blastogenesis in peripheral lymphocytes from control individuals. Environ Health Persp 1993;101(Suppl 3):83-8.10.1289/ehp.93101s383Search in Google Scholar

Sharma T, Das BC. Higher incidence of spontaneous sister-chromatid exchanges (SCEs) and X-ray-induced chromosome aberrations in peripheral blood lymphocytes during pregnancy. Mutat Res 1986;174:27-33.10.1016/0165-7992(86)90073-4Search in Google Scholar

Vijayalaxmi, Evans HJ. In vivo and in vitro effects of cigarette smoke on chromosomal damage and sister chromatid exchange in human peripheral blood lymphocytes. Mutat Res 1982;92:321-32.10.1016/0027-5107(82)90234-2Search in Google Scholar

Obe G, Vogt HJ, Madle S, Fahning A, Heller WD. Doubleblind study on the effect of cigarette smoking on the chromosomes of human peripheral blood lymphocytes in vivo.Mutat Res 1982;92:309-19.10.1016/0027-5107(82)90233-0Search in Google Scholar

Tucker JD, Ashworth LK, Johnston GR, Allen NA, Carrano AV. Variation in human lymphocyte sister-chromatid exchange frequency: result of a long-term longitudinal study. Mutat Res 1988;204:435-44.10.1016/0165-1218(88)90039-0Search in Google Scholar

Reidy JA, Annest JL, Chen ATL, Welty TK. Increased sister chromatid exchange associated with smoking and coffee consumption. Environ Mol Mutagen 1988;12:311-8.10.1002/em.2860120305Search in Google Scholar

Bonassi S, Bolognesi C, Abbondandolo A, Barale R, Bigatti P, Camurri L, Dalprà L, De Ferrari M, Forni A, Lando C, Padovani P, Pasquini R, Stella M, Puntoni R. Influence of sex on cytogenetic end points - evidence from a large human sample and review of the literature. Cancer Epidemiol Biomarkers Prev 1995;4:671-9.Search in Google Scholar

Slapšyte G, Jankauskiene A, Mieraiskiene J, Lazutka JR. Cytogenetic analysis of peripheral blood lymphocytes of children treated with nitrofurantion for recurrent urinary tract infection. Mutagenesis 2002;17:31-5.10.1093/mutage/17.1.31Search in Google Scholar

Gutierrez S, Carbonell E, Galofre P, Creus A, Marcos R. Low sensitivity of the sister chromatid exchange assay to detect the genotoxic effects of radioiodine therapy. Mutagenesis 1999;14:221-6.10.1093/mutage/14.2.221Search in Google Scholar

Jacobson-Kram D. The effects of diagnostic ultrasound on sister chromatid exchange frequencies: a review of the recent literature. J Clin Ultrasound 1984;12:5-10.10.1002/jcu.1870120104Search in Google Scholar

Garaj-Vrhovac V, Kopjar N. Cytogenetic monitoring of cardiology unit hospital workers exposed to ultrasound. J Appl Toxicol 2000;20:259-64.10.1002/1099-1263(200007/08)20:4<259::AID-JAT655>3.0.CO;2-NSearch in Google Scholar

Atalay F, Baltaci V, Alpas I, Savas I, Atikcan S, Balci S. Sister chromatid exchange rate from pleural fluid cells in patient with malignant mesothelioma. Mutat Res 2000;465:159-63.10.1016/S1383-5718(99)00224-7Search in Google Scholar

Cortes-Guttierez EI, Cerda-Flores RM, Leal-Garza CH. Sister chromatid exchanges in peripheral lymphocytes from women with carcinoma of the uterine cervix. Cancer Genet Cytogenet 2000;122:121-3.10.1016/S0165-4608(00)00286-7Search in Google Scholar

Cottliar A, Fundia A, Boerr L, Sambuelli A, Negreira S, Gil A, Gomez JC, Chopita N, Bernedo A, Slavutsky I. High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis. Am J Gastroenterol 2000;95:2301-7.10.1111/j.1572-0241.2000.02315.xSearch in Google Scholar

Carbonell E, Demopoulos NA, Stefanou G, Psaraki K, Parry EM, Marcos R. Cytogenetic analysis in peripheral lymphocytes of cancer patients treated with cytostatic drugs: Results from an EC Collaborative Study. Anticancer Drugs 1996;7:514-9.10.1097/00001813-199607000-00003Search in Google Scholar

Kopjar N, Milas I, Garaj-Vrhovac V, Gamulin M. Cytogenetic outcomes of adjuvant chemotherapy in non-target cells of breast cancer patients. Hum Exp Toxicol 2007;26:391-9.10.1177/0960327106076812Search in Google Scholar

Norppa H, Bonassi S, Hansteen I-L, Hagmar L, Strömberg U, Rössner P, Boffeta P, Lindholm C, Gundy S, Lazutka J, Cebulska-Wasilewska A, Fabianoca E, Šram RJ, Knudsen LE, Barale R, Fučić A. Chromosomal aberrations and SCEs as biomarkers of cancer risk. Mutat Res 2006;600:37-45.10.1016/j.mrfmmm.2006.05.030Search in Google Scholar

Natarajan AT, Boei JJ, Darroudi F, Van Diemen PCM, Dulout F, Hande MP, Ramalho AT. Current cytogenetic methods for detecting exposure and effects of mutagens and carcinogens. Environ Health Persp 1996;104(Suppl 3):445-8.10.1289/ehp.96104s3445Search in Google Scholar

Bender MA, Awa AA, Brooks AL, Evans HJ, Groer PO, Littlefield LG, Pereira C, Preston J, Wachholz BW. Current status of cytogenetic procedures to detect and quantify previous exposure to radiation. Mutat Res 1988;196:103-59.10.1016/0165-1110(88)90017-6Search in Google Scholar

International Atomic Energy Agency (IAEA). Cytogenetic Analysis for Radiation Dose Assessment. Technical Report Series no. 405. Vienna: IAEA; 2001.Search in Google Scholar

Kopjar N, Želježić D, Garaj-Vrhovac V. Evaluation of DNA damage in the white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test. Acta Biochim Pol 2006;53:321-36.10.18388/abp.2006_3346Search in Google Scholar

Hagmar L, Bonassi S, Strömberg U, Mikoczy Z, Lando C, Hansten I-L, Huici Montagud A, Knudsen L, Norppa H, Reuterwall C, Tinnerberg H, Brøgger A, Forni A, Högstedt B, Lambert B, Mitelman F, Nordenson I, Salomaa S, Skerfving S. Cancer predictive value of cytogenetic markers used in occupational health surrveilance programs: a report from an ongoing study by the European Study Group on Cytogenetic Biomarkers and Health. Mutat Res 1998;405:171-8.10.1016/S0027-5107(98)00134-1Search in Google Scholar

Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 2003;534:65-75.10.1016/S1383-5718(02)00249-8Search in Google Scholar

Salama S, Serrana M, Au WW. Biomonitoring using accessible human cells for exposure and health risk assessment. Mutat Res 1999;436:99-112.10.1016/S1383-5742(98)00021-0Search in Google Scholar

Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659:93-108.10.1016/j.mrrev.2008.03.007Search in Google Scholar

Norppa H, Luomahaara S, Heikanen H, Roth S, Sorsa M, Renzi L, Lindholm C. Micronucleus assay in lymphocytes as a tool to biomonitor human exposure to aneuploidogens and clastogens. Environ Health Persp 1993;101(Suppl 3):139-43.10.1289/ehp.93101s3139Search in Google Scholar

Kirsch Volders M, Elhajouji A, Cundari E, Vanhumelen P. The in vitro micronucleus test - a multiendpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat Res 1997;392:19-30.10.1016/S0165-1218(97)00042-6Search in Google Scholar

Fenech M, Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res 1985;147:29-36.10.1016/0165-1161(85)90015-9Search in Google Scholar

Norppa H, Falck GCM. What do human micronuclei contain? Mutagenesis 2003;18:221-33.10.1093/mutage/18.3.22112714687Search in Google Scholar

Schreiber GA, Beisker W, Braselmann H, Bauchinger M, Bögl KW, Nüsse M. Automated flow cytometric micronucleus assay for human lymphocytes. Int J Radiat Biol 1992;62:695-709.10.1080/095530092145526511362763Search in Google Scholar

Verschaeve L, Vanderkerken M, Kirsch-Volders M. Cbanding as a simple tool to discriminate micronuclei induced by clastogens and aneugens. Stain Technol 1988;63:351-4.10.3109/105202988091076103250009Search in Google Scholar

Mateuca R, Lombaert N, Aka PV, Decodier I, Kirsch-Volders M. Chromosomal changes: induction, detection methods and applicability in human biomonitoring. Biochemie 2006;88:1515-31.10.1016/j.biochi.2006.07.00416919864Search in Google Scholar

Fenech M. The cytokinesis-block micronucleus technique: A detailed description of the method and its application to genotoxicity studies in human population. Mutat Res 1993;285:35-44.10.1016/0027-5107(93)90049-LSearch in Google Scholar

Fenech M, Neville S, Rinaldi J. Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesisblocked lymphocytes. Mutat Res 1994;313:203-7.10.1016/0165-1161(94)90050-7Search in Google Scholar

Tucker JD, Nath J, Hando JC. Activation status of the X chromosome in human micronucleated lymphocytes. Hum Genet 1996;97:471-5.10.1007/BF02267069Search in Google Scholar

Fenech M, Morley AA. Kinetochore detection in micronuclei: an alternative method for measuring chromosome loss. Mutagenesis 1989;4:98-104.10.1093/mutage/4.2.98Search in Google Scholar

Norppa H, Luomahaara S, Heikanen H, Roth S, Sorsa M, Renzi L, Lindholm C. Micronucleus assay in lymphocytes as a tool to biomonitor human exposure to aneuploidogens and clastogens. Environ Health Persp 1993;101(Suppl 3):139-43.10.1289/ehp.93101s3139Search in Google Scholar

Martínez-Pérez LM, Cerda-Flores RM, Gallegos-Cabriales EC, Dávila-Rodríguez MI, Ibarra-Costilla E, Cortés-Gutiérrez EI. Frequency of micronuclei in Mexicans with type 2 diabetes mellitus. Prague Med Rep 2007;108:248-55.Search in Google Scholar

Masjedi MR, Heidary A, Mohammadi F, Velayati AA, Dokouhaki P. Chromosomal aberrations and micronuclei in lymphocytes of patients before and after exposure to antituberculosis drugs. Mutagenesis 2000;15:489-94.10.1093/mutage/15.6.489Search in Google Scholar

Milošević-Đorđević O, Grujičić D, Marinković D, Arsenijević S, Banković S. Effect of various doses of gestogens on micronuclei frequency in human peripheral blood lymphocytes of pregnant women. Hum Reprod 2003;18:433-6.10.1093/humrep/deg068Search in Google Scholar

Cochran ST, Khodadoust A, Norman A. Cytogenetic effects of contrast material in patients undergoing excretory urography. Radiology 1980;136:43-6.10.1148/radiology.136.1.7384520Search in Google Scholar

Norman A, Cochran S, Bass D, Roe D. Effects of age, sex and diagnostic X-rays on chromosome damage. Int J Radiat Biol 1984;46:317-21.10.1080/09553008414551451Search in Google Scholar

Larramendy ML, Knuutila S. Increased frequency of micronuclei in B and T8 lymphocytes from smokers. Mutat Res 1991;259:189-95.10.1016/0165-1218(91)90052-NSearch in Google Scholar

Bonassi S, Neri M, Lando C, Ceppi M, Lin Y, Chang WP, Holland N, Kirsch-Volders M, Zeiger E, Fenech M. The HUMN collaborative group. Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project. Mutat Res 2003;543:155-66.10.1016/S1383-5742(03)00013-9Search in Google Scholar

Bonassi S, Znaor A, Ceppi M, Lando C, Chang WP, Holland N, Kirsch-Volders M, Zeiger E, Ban S, Barale R, Bigatti P, Bolognesi C, Cebulska-Wasilewska A, Fabianova E, Fučić A, Hagmar L, Joksić G, Martelli A, Migliore L, Mirkova E, Scarfi MR, Zijno A, Norppa H, Fenech M. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 2007;28:625-31.10.1093/carcin/bgl177Search in Google Scholar

Rojas E, Lopez MC, Valverde M. Single cell electrophoresis assay: methodology and applications. J Chrom B 1999;722:225-54.10.1016/S0378-4347(98)00313-2Search in Google Scholar

Singh NP. Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res 2000;455:111-27.10.1016/S0027-5107(00)00075-0Search in Google Scholar

Plappert U, Raddatz K, Roth S, Fliedner TM. DNA damage detection in man after radiation exposure - the comet assay - its possible application for human monitoring. Stem Cells 1995:13:215-22.Search in Google Scholar

Singh NP, McCoy MT, Tice RR, Schneider LL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91.10.1016/0014-4827(88)90265-0Search in Google Scholar

Olive PL. Applications of the comet assay in radiobiology. Int J Radiat Biol 1999;75:395-405.10.1080/09553009914031110331844Search in Google Scholar

Collins A, Dušinská M, Gedik C, Štetina R. Oxidative damage to DNA: Do we have a reliable marker? Environ Health Persp 1996;104(Suppl 3):465-9.10.1289/ehp.96104s346514696448781365Search in Google Scholar

Olive PL, Banath JP, Durand RE. Heterogeneity in radiationinduced DNA damage and repair in tumor and normal cells using the "comet" assay. Radiat Res 1990;122:86-94.10.2307/3577587Search in Google Scholar

Wojewódzka M, Grądzka I, Buraczewska I. Modified neutral comet assay for human lymphocytes. Nukleonika 2002;47:1-5.Search in Google Scholar

Pfeiffer P, Goedecke W, Obe G. Mechanisms of DNA double strand break repair and their potential to induce chromosomal aberrations. Mutagenesis 2000;15:289-302.10.1093/mutage/15.4.28910887207Search in Google Scholar

Speit G, Schütz P, Bonzheim I, Trenz K, Hoffmann H. Sensitivity of the FPG protein towards alkylation damage in the comet assay. Toxicol Lett 2004;146:151-8.10.1016/j.toxlet.2003.09.01014643967Search in Google Scholar

Smith CC, O'Donovan MR, Martin EA. hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII. Mutagenesis 2006;21:185-90.10.1093/mutage/gel019Search in Google Scholar

Miyamae Y, Zaizen K, Ohara K, Mine Y, Sasaki YF. Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens. Mutat Res 1998;415:229-35.10.1016/S1383-5718(97)00192-7Search in Google Scholar

Tice RR. The single cell gel / comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells. U: Phillips DH, Vennit S, urednici. Environmental Mutagenesis. Oxford: Bioscientific; 1995. str. 315-39.Search in Google Scholar

Kaminskas E, Li JC. Repair of DNA damage induced by oxygen radicals in human non-proliferating and proliferating lymphocytes. Mutat Res 1992;274:103-10.10.1016/0921-8777(92)90057-ASearch in Google Scholar

Šalagovič J, Maes A, Van Gorp U, Verschaeve L, Kalina I. The cell cycle positions Influence DNA migration as measured with the alkaline comet assay in stimulated human lymphocytes. Folia Biol (Praha) 1997;43:79-82.Search in Google Scholar

Villani P, Altavista PL, Castaldi L, Leter G, Cordelli E. Analysis of DNA oxidative damage related to cell proliferation. Mutat Res 2000;464:229-37.10.1016/S1383-5718(99)00192-8Search in Google Scholar

Fortini P, Raspaglio G, Falchi M, Dogliotti E. Analysis of DNA alkylation damage and repair in mammalian cells by the comet assay. Mutagenesis 1996;11:169-75.10.1093/mutage/11.2.169Search in Google Scholar

Betti C, Davini T, Giannessi L, Loprieno N, Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat Res 1994;307:323-33.10.1016/0027-5107(94)90306-9Search in Google Scholar

Wojewódzka M, Kruszewski M, Iwanenko T, Collins AR, Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage. Mutat Res 1998;416:21-35.10.1016/S1383-5718(98)00073-4Search in Google Scholar

Mendoza-Núñez VM, Sánchez-Rodríguez MA, Retana-Ugalde R, Vargas-Guadarrama LA, Altamirano-Lozano MA. Total antioxidant levels, gender, and age as risk factors for DNA damage in lymphocytes of the elderly. Mech Ageing Dev 2001;122:835-47.10.1016/S0047-6374(01)00240-8Search in Google Scholar

Bajpayee M, Dhawan A, Parmar D, Kumar Pendey A, Mathur N, Seth PK. Gender-related differences in basal DNA damage in lymphocytes of a healthy Indian population using the alkaline comet assay. Mutat Res 2002;520:83-91.10.1016/S1383-5718(02)00175-4Search in Google Scholar

Frenzilli G, Betti C, Davini T, Desideri M, Fornai E, Giannessi L, Maggiorelli F, Paoletti P, Barale R. Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis. Mutat Res 1997;375:117-23.10.1016/S0027-5107(97)00007-9Search in Google Scholar

Zhu CQ, Lam TH, Jiang CQ, Wei BX, Lou X, Liu WW, Lao XQ, Chen YH. Lymphocyte DNA damage in cigarette factory workers measured by the comet assay. Mutat Res 1999;444:1-6.10.1016/S1383-5718(99)00097-2Search in Google Scholar

Singh NP, Danner DB, Tice RR, Pearson JD, Brant LJ, Morrell CH, Schneider EL. Basal DNA damage in individual human lymphocytes with age. Mutat Res 1991;256:1-6.10.1016/0921-8734(91)90026-8Search in Google Scholar

Piperakis SM, Visvardis EE, Sagnou M, Tassiou AM. Effects of smoking and aging on oxidative DNA damage of human lymphocytes. Carcinogenesis 1998;19:695-8.10.1093/carcin/19.4.695Search in Google Scholar

Kopjar N, Želježić D, Garaj-Vrhovac V. Evaluation of DNA damage in the white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test. Acta Biochim Pol 2006;53:321-36.10.18388/abp.2006_3346Search in Google Scholar

Hellman B, Friis L, Vaghef H, Edling C. Alkaline single cell gel electrophoresis and human biomonitoring for genotoxicity: a study on subjects with residential exposure to radon. Mutat Res 1999;442:121-32.10.1016/S1383-5718(99)00083-2Search in Google Scholar

Moller P, Wallin H, Holst E, Knudsen LE. Sunlight-induced DNA damage in human mononuclear cells. FASEB J 2002;16:45-53.10.1096/fj.01-0386comSearch in Google Scholar

Verschaeve L, Koppen G, Gorp UV, Schoeters G, Jacobs G, Zwijzen C. Seasonal variations in spontaneous levels of DNA damage; implication in the risk assessment of environmental chemicals. J Appl Toxicol 2007;27:612-20.10.1002/jat.1244Search in Google Scholar

Gutiérrez S, Carbonell E, Galofré P, Creus A, Marcos R. The alkaline single-cell gel electrophoresis (SCGE) assay applied to the analysis of radiation-induced DNA damage in thyroid cancer patients treated with ¹³¹I. Mutat Res 1998;413:111-9.10.1016/S1383-5718(98)00010-2Search in Google Scholar

Dantas FJ, de Mattos JC, Moraes MO, Boasquevisques E, Rodrigues MP, Lage CA, Cabral-Neto JB, Leitão AC, Bernardo-Filho M, Bezerra RJ, Carvalho JJ, Caldeira-de-Araujo A. DNA damage in peripheral blood nuclear cells assessed by comet assay from individuals submitted to scintigraphic examinations. Cell Mol Biol 2002;48:789-91.Search in Google Scholar

Biri A, Civelek E, Karahalil B, Sardaş S. Assessment of DNA damage in women using oral contraceptives. Mutat Res 2002;521:113-9.10.1016/S1383-5718(02)00217-6Search in Google Scholar

Milković Đ, Garaj-Vrhovac V, Ranogajec-Komor M, Miljanić S, Gajski G, Knežević Ž, Beck N. Primary DNA damage assessed with the comet assay and comparison to the absorbed dose of diagnostic X-rays in children. Int J Toxicol 2009;5:405-16.10.1177/109158180934477519815846Search in Google Scholar

Mastaloudis A, Yu T-W, O'Donnel RP, Frei B, Dashwood RH, Traber MG. Endurance exercise results in DNA damage as detected by the comet assay. Free Radical Biol Med 2004;36:966-75.10.1016/j.freeradbiomed.2004.01.012Search in Google Scholar

Møller P, Knudsen LE, Frentz G, Dybdahl M, Wallin H, Nexø BA. Seasonal variation of DNA damage and repair in patients with non-melanoma skin cancer and referents with and without psoriasis. Mutat Res 1998;407:25-34.10.1016/S0921-8777(97)00057-8Search in Google Scholar

Mozaffarieh M, Schoetzau A, Sauter M, Grieshaber M, Orgül S, Golubnitschaja O, Flammer J. Comet assay analysis of single-stranded DNA breaks in circulating leukocytes of glaucoma patients. Mol Vis 2008;14:1584-8.Search in Google Scholar

Bilgici B, Bedir A, Şentürk N, Alvur M, Aydin F, Turanli AY. Genotoxicity assessment using comet assay in Behcet's disease patients. Mutat Res 2005;578:170-4.10.1016/j.mrfmmm.2005.05.01116019038Search in Google Scholar

Hininger I, Chollat-Namy A, Sauvagio S, Osman M, Faure H, Cadet J, Favier A, Rousell A-M. Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers. Mutat Res 2004;558:75-80.10.1016/j.mrgentox.2003.11.00415036121Search in Google Scholar

Rapp A, Hausmann M, Greulich KO. The comet-FISH technique: a tool for detection of specific DNA damage and repair. Methods Mol Biol 2005;291:107-19.Search in Google Scholar