Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Varanda EA, Tavares DC. Radioprotection: Mechanism and radioprotective agents including honey bee venom. Venom Anim Toxins 1998;4:5-21.10.1590/S0104-79301998000100002Search in Google Scholar

Varanda EA, Monti R, Tavares DC. Inhibitory effect of propolis and bee venom on the mutagenicity of some direct- and indirect-acting mutagens. Teratogen Carcinogen Mutagen 1999;19:403-13.10.1002/(SICI)1520-6866(1999)19:6<403::AID-TCM4>3.0.CO;2-2Search in Google Scholar

Nam KW, Je KH, Lee JH, Han HJ, Lee HJ, Kang SK, Mar W. Inhibition of COX-2 activity and proinflammatory cytokines (TNF-alpha and IL-1beta) production by water-soluble subfractionated parts from bee (Apis mellifera) venom. Arch Pharm Res 2003;26:383-8.10.1007/BF02976695Search in Google Scholar

Kim HW, Kwon YB, Ham TW, Roh DH, Yoon SY, Lee HJ, Han HJ, Yang IS, Beitz AJ, Lee JH. Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rats. J Vet Med Sci 2003;65:349-55.10.1292/jvms.65.349Search in Google Scholar

Orsolic N, Sver L, Verstovsek S, Terzic S, Basic I. Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom. Toxicon 2003;41:861-70.10.1016/S0041-0101(03)00045-XSearch in Google Scholar

Liu X, Chen D, Xie L, Zhang R. Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo. J Pharm Pharmacol 2002;54:1083-9.10.1211/002235702320266235Search in Google Scholar

Jang MH, Shin MC, Lim S, Han SM, Park HJ, Shin I. Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299. J Pharmacol Sci 2003;91:95-104.10.1254/jphs.91.95Search in Google Scholar

Yin CS, Lee HJ, Hong SJ, Chung JH, Koh HG. Microarray analysis of gene expression in chondrosarcoma cells treated with bee venom. Toxicon 2005;45:81-91.10.1016/j.toxicon.2004.09.017Search in Google Scholar

Hu H, Chen D, Li Y, Zhang X. Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo. J Pharm Pharmacol 2006;58:83-9.10.1211/jpp.58.1.0010Search in Google Scholar

Moon DO, Park SY, Heo MS, Kim KC, Park C, Ko WS. Key regulators in bee venom-induced apoptosis are Bcl-2 and caspase-3 in human leukemic U937 cells through downregulation of ERK and Akt. Int Immunopharmacol 2006;6:1796-807.10.1016/j.intimp.2006.07.027Search in Google Scholar

Han S, Lee K, Yeo J, Kweon H, Woo S, Lee M, Baek H, Kim S, Park K. Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS. J Ethnopharmacol 2007;111:176-81.10.1016/j.jep.2006.11.008Search in Google Scholar

Habermann E. Bee and wasp venoms. Science 1972;177:314-22.10.1126/science.177.4046.314Search in Google Scholar

Dotimas EM, Hamid KR, Hider RC, Ragnarsson U. Isolation and structure analysis of bee venom mast cell degranulating peptide. Biochim Biophys Acta 1987;911:285-93.10.1016/0167-4838(87)90069-0Search in Google Scholar

Neumann W, Habermann E. Characterization of the substances of the bee venom. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol 1954;222:267-87.Search in Google Scholar

Vogel H. Incorporation of melittin into phosphatidylcholine bilayers. Study of binding and conformational changes. FEBS Lett 1981;134:37-42.10.1016/0014-5793(81)80545-5Search in Google Scholar

Gauldie J, Hanson JM, Rumjanek FD, Shipolini RA, Vernon CA. The peptide components of bee venom. Eur J Biochem 1976;1:369-76.10.1111/j.1432-1033.1976.tb10030.xSearch in Google Scholar

Gevod VS, Birdi KS. Melittin and the 8-26 fragment. Differences in ionophoric properties as measured by monolayer method. Biophys J 1984;45:1079-83.10.1016/S0006-3495(84)84255-1Search in Google Scholar

Habermann E. Chemistry, pharmacology, and toxicology of bee, wasp and hornet venoms. In: Bucherl W, and Buckley EE, editors. Venomous animals and their venoms. Vol. 3. New York (NY): Academic Press; 1971. p. 61-93.10.1016/B978-0-12-138903-1.50009-0Search in Google Scholar

Fletcher JE, Jiang MS. Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin. Toxicon 1993;31:669-95.10.1016/0041-0101(93)90375-SSearch in Google Scholar

Gajski G, Ravlic S, Capuder Z, Garaj-Vrhovac V. Use of sensitive methods for detection of DNA damage on human lymphocytes exposed to p,p'-DDT: Comet assay and new criteria for scoring micronucleus test. J Environ Sci Health B 2007;42:607-13.10.1080/0360123070146544517701695Search in Google Scholar

Gajski G, Garaj-Vrhovac V. Application of cytogenetic endpoints and comet assay on human lymphocytes treated with atorvastatin in vitro. J Environ Sci Health A Tox Hazard Subst Environ Eng 2008;43:78-85.10.1080/1093452070175006618161561Search in Google Scholar

Gajski G, Garaj-Vrhovac V, Orescanin V. Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay. Toxicol Appl Pharmacol 2008;231:85-93.10.1016/j.taap.2008.03.013Search in Google Scholar

Singh NP, McCoy MT, Tice RR, Schneider LL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91.10.1016/0014-4827(88)90265-0Search in Google Scholar

Fenech M, Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res 1985;147:29-36.10.1016/0165-1161(85)90015-9Search in Google Scholar

Duke RC, Cohen JJ. Morphological and biochemical assays of apoptosis. In: Coligan JE, Kruisbeal AM, editors. Current protocols in immunology. New York (NY): John Willey & Sons; 1992. p. 1-3.Search in Google Scholar

Singh NP. Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res 2000;455:111-27.10.1016/S0027-5107(00)00075-0Search in Google Scholar

Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 2004;26:249-61.10.1385/MB:26:3:249Search in Google Scholar

Betti C, Davini T, Giannessi L, Loprieno N, Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat Res 1994;307:323-33.10.1016/0027-5107(94)90306-9Search in Google Scholar

Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E. HUMN project: detailed description of scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 2003;534:65-75.10.1016/S1383-5718(02)00249-8Search in Google Scholar

Henderson L, Wolfreys A, Fedyk J, Bourner C, Windebank S. The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 1998;13:89-94.10.1093/mutage/13.1.89Search in Google Scholar

Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35:206-16.10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-JSearch in Google Scholar

Hellman B, Vaghef H, Boström B. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat Res 1995;336:123-31.10.1016/0921-8777(94)00047-ASearch in Google Scholar

Kumaravel TS, Vilhar B, Faux SP, Jha AN. Comet Assay measurements: a perspective. Cell Biol Toxicol 2009;25:53-64.10.1007/s10565-007-9043-9Search in Google Scholar

Olive PL. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Radiat Biol 1999;75:395-405.10.1080/095530099140311Search in Google Scholar

Piperakis SM, Visvardis EE, Tassiou AM. Comet assay for nuclear DNA damage. Methods Enzymol 1999;300:184-94.10.1016/S0076-6879(99)00125-1Search in Google Scholar

Kirsch-Volders M, Sofuni T, Aardema M, Albertini S, Eastmond D, Fenech M, Ishidate MJr, Kirchner S, Lorge E, Morita T, Norppa H, Surralles J, Vanhauwaert A, Wakata A. Report from the in vitro micronucleus assay working group. Mutat Res 2003;540:153-63.10.1016/j.mrgentox.2003.07.005Search in Google Scholar

Fenech M. The in vitro micronucleus technique. Mutat Res 2000;455:81-95.10.1016/S0027-5107(00)00065-8Search in Google Scholar

Fenech M. Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction and cell death. Mutat Res 2006;600:58-66.10.1016/j.mrfmmm.2006.05.02816822529Search in Google Scholar

Garaj-Vrhovac V, Gajski G, Ravlić S. Efficacy of HUMN criteria for scoring the micronucleus assay in human lymphocytes exposed to a low concentration of p,p'-DDT. Braz J Med Biol Res 2008;41:473-6.10.1590/S0100-879X200800500002718592119Search in Google Scholar

Garaj-Vrhovac V, Đurinec M, Kojpar N, Oreščanin V. A survey on the cytogenetic status of the Croatian general population by use of the cytokinesis-block micronucleus assay. Mutat Res 2008;649:91-100.10.1016/j.mrgentox.2007.08.00818029222Search in Google Scholar

Gajski G, Garaj-Vrhovac V. Genotoxic potential of bee venom (Apis mellifera) on human peripheral blood lymphocytes in vitro using single cell gel electrophoresis assay. J Environ Sci Health A Tox Hazard Subst Environ Eng 2008;43:1279-87.10.1080/1093452080217786218642151Search in Google Scholar

Lee YJ, Kang SJ, Kim BM, Kim YJ, Woo HD, Chung HW. Cytotoxicity of honeybee (Apis mellifera) venom in normal human lymphocytes and HL-60 cells. Chem Biol Interact 2007;169:189-97.10.1016/j.cbi.2007.06.03617658502Search in Google Scholar

Ip SW, Liao SS, Lin SY, Lin JP, Yang JS, Lin ML, Chen GW, Lu HF, Lin MW, Han SM, Chung JG. The role of mitochondria in bee venom-induced apoptosis in human breast cancer MCF7 cells. In Vivo 2008;22:237-45.Search in Google Scholar

Stuhlmeier KM. Apis mellifera venom and melittin block neither NF-kappa B-p50-DNA interactions nor the activation of NF-kappa B, instead they activate the transcription of proinflammatory genes and the release of reactive oxygen intermediates. J Immunol 2007;179:655-64.10.4049/jimmunol.179.1.65517579088Search in Google Scholar