<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Acute pancreatitis represents an acute nonbacterial inflammation of the pancreas caused by a premature and ectopic activation of pancreatic digestive enzymes. Two of the most important genes in pancreatic autodigestion, PRSS1 and SPINK1, were implicated in the earliest discoveries of the genetic background of pancreatitis. However, the distribution of their variations displays interethnic variability, which could significantly affect the magnitude of their proposed effects on this disease worldwide. The aim of the present study was to investigate the distribution of the most important functional variations of PRSS1 (86A>T and 365G>A) and SPINK1 (101A>G), and their influence on the clinical course of acute pancreatitis in Serbian patients. The study enrolled 81 subjects, the severity of disease course was determined using the Atlanta Classification system, and the genotyping was conducted using a PCR-RFLP method. PRSS1 86A>T and 365G>A SNPs were not observed in the study population, while SPINK1 101A>G was present with the frequency of 0.62% (95% CI: 0.00, 3.83%). Due to extremely low frequencies or absences of examined variations, the proposed effect of these SNPs on the severity of acute pancreatitis could not be confirmed. The results do not support routine genotyping of either PRSS1 or SPINK1 in Serbs.</p></abstract>

Acute pancreatitis represents an acute nonbacterial inflammation of the pancreas caused by a premature and ectopic activation of pancreatic digestive enzymes. Two of the most important genes in pancreatic autodigestion, PRSS1 and SPINK1, were implicated in the earliest discoveries of the genetic background of pancreatitis. However, the distribution of their variations displays interethnic variability, which could significantly affect the magnitude of their proposed effects on this disease worldwide. The aim of the present study was to investigate the distribution of the most important functional variations of PRSS1 (86A>T and 365G>A) and SPINK1 (101A>G), and their influence on the clinical course of acute pancreatitis in Serbian patients. The study enrolled 81 subjects, the severity of disease course was determined using the Atlanta Classification system, and the genotyping was conducted using a PCR-RFLP method. PRSS1 86A>T and 365G>A SNPs were not observed in the study population, while SPINK1 101A>G was present with the frequency of 0.62% (95% CI: 0.00, 3.83%). Due to extremely low frequencies or absences of examined variations, the proposed effect of these SNPs on the severity of acute pancreatitis could not be confirmed. The results do not support routine genotyping of either PRSS1 or SPINK1 in Serbs.

Lack Of <italic>PRSS1</italic> And <italic>SPINK1</italic> Polymorphisms In Serbian Acute Pancreatitis Patients

Faculty of Medical Sciences, University of Kragujevac, Serbia

Experimental and Applied Biomedical Research (EABR)

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Lack Of  And  Polymorphisms In Serbian Acute Pancreatitis Patients

Acute pancreatitis represents an acute nonbacterial inflammation of the pancreas caused by a premature and ectopic activation of pancreatic digestive enzymes....

Lack Of PRSS1 And SPINK1 Polymorphisms In Serbian Acute Pancreatitis Patients

Published Online: Oct 14, 2015

Page range: 201 - 206

Received: Dec 11, 2014

Accepted: Mar 03, 2015

DOI: https://doi.org/10.1515/sjecr-2015-0026

Keywords
PRSS1, SPINK1, genetic polymorphism, acute pancreatitis, Serbian

© 2015 Ivan Radosavljevic et al., published by De Gruyter Open

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Lack Of PRSS1 And SPINK1 Polymorphisms In Serbian Acute Pancreatitis Patients

Published Online: Oct 14, 2015

Page range: 201 - 206

Received: Dec 11, 2014

Accepted: Mar 03, 2015

DOI: https://doi.org/10.1515/sjecr-2015-0026

KeywordsPRSS1, SPINK1, genetic polymorphism, acute pancreatitis, Serbian

© 2015 Ivan Radosavljevic et al., published by De Gruyter Open

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Keywords
PRSS1, SPINK1, genetic polymorphism, acute pancreatitis, Serbian