<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Eucalyptus globulus</italic> is the main hardwood species grown in pulpwood plantations in temperate regions of the world. We have cloned six genes influencing wood quality including the LIM domain transcription factor (LIM), 4-coumarate-CoA ligase (4CL), coniferaldehyde 5-hydroxylase (CAld5H) and the three catalytic units of cellulose synthase (CesA), from <italic>E. globulus</italic>. The transcript abundances of LIM in basal stems of ten independent <italic>E. globulus</italic> lines showed similar patterns to those of 4CL. We investigated the correlation between gene transcript abundances and wood qualities such as Klason lignin (KL) content, syringaldehyde/vanillin (S/V) ratio and holocellulose (HC) content. Expression of the LIM and 4CL were positively correlated with KL content. A highly significant positive correlation was observed between CAld5H expression and S/V ratio. Furthermore, a ratio of the sum of the transcript abundances of three CesA1, CesA2 and CesA3 to 4CL showed a positive correlation with a ratio of HC/KL content that positively correlated with the chemically extracted fiber content in this woody plant.</p></abstract>

Eucalyptus globulus is the main hardwood species grown in pulpwood plantations in temperate regions of the world. We have cloned six genes influencing wood quality including the LIM domain transcription factor (LIM), 4-coumarate-CoA ligase (4CL), coniferaldehyde 5-hydroxylase (CAld5H) and the three catalytic units of cellulose synthase (CesA), from E. globulus. The transcript abundances of LIM in basal stems of ten independent E. globulus lines showed similar patterns to those of 4CL. We investigated the correlation between gene transcript abundances and wood qualities such as Klason lignin (KL) content, syringaldehyde/vanillin (S/V) ratio and holocellulose (HC) content. Expression of the LIM and 4CL were positively correlated with KL content. A highly significant positive correlation was observed between CAld5H expression and S/V ratio. Furthermore, a ratio of the sum of the transcript abundances of three CesA1, CesA2 and CesA3 to 4CL showed a positive correlation with a ratio of HC/KL content that positively correlated with the chemically extracted fiber content in this woody plant.

Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in <italic>Eucalyptus globulus</italic>

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in 

Eucalyptus globulus is the main hardwood species grown in pulpwood plantations in temperate regions of the world. We have cloned six genes influencing wood...

Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in Eucalyptus globulus

Published Online: Aug 05, 2017

Page range: 288 - 296

Received: Jul 21, 2011

DOI: https://doi.org/10.1515/sg-2011-0038

Keywords
LIM domain transcription factor, CAld5H, CesA, 4CL,

© 2011 N. Negishi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in Eucalyptus globulus

Published Online: Aug 05, 2017

Page range: 288 - 296

Received: Jul 21, 2011

DOI: https://doi.org/10.1515/sg-2011-0038

KeywordsLIM domain transcription factor, CAld5H, CesA, 4CL,

© 2011 N. Negishi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
LIM domain transcription factor, CAld5H, CesA, 4CL,