<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Araucaria araucana</italic> (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we report the development and characterization of a set of microsatellite markers in the species by means of the microsatellite-AFLP (M-AFLP) technique. A total of 25 M-AFLP derived bands, showing a typical microsatellite pattern, were selected and sequenced. Of these, 12 that contained microsatellite sequences, were used for primer extension. Six of the resulting SSR markers provided easily interpretable patterns and were used to investigate the level of genetic diversity in two populations of <italic>A. araucana</italic>. A total of 43 alleles were amplified. The mean overall loci of observed and expected heterozygosities for the Conguillio and Villa Araucaria populations were 0.322 and 0.443, respectively. The primers presented in this study may provide useful information for the establishment of a conservation strategy in the species.</p></abstract>

Araucaria araucana (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we report the development and characterization of a set of microsatellite markers in the species by means of the microsatellite-AFLP (M-AFLP) technique. A total of 25 M-AFLP derived bands, showing a typical microsatellite pattern, were selected and sequenced. Of these, 12 that contained microsatellite sequences, were used for primer extension. Six of the resulting SSR markers provided easily interpretable patterns and were used to investigate the level of genetic diversity in two populations of A. araucana. A total of 43 alleles were amplified. The mean overall loci of observed and expected heterozygosities for the Conguillio and Villa Araucaria populations were 0.322 and 0.443, respectively. The primers presented in this study may provide useful information for the establishment of a conservation strategy in the species.

Primer Note: Microsatellite-AFLP development for <italic>Araucaria araucana</italic> (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests

Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel

Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario (ceiA3)

Istituto di Biología Agroambientale e Forestale (IBAF)

Departamento de Manejo de Bosques y Medioambiente, Facultad de Ciencias Forestales

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primer Note: Microsatellite-AFLP development for  (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests

Araucaria araucana (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we...

Primer Note: Microsatellite-AFLP development for Araucaria araucana (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests

Published Online: Aug 05, 2017

Page range: 285 - 288

Received: Jun 14, 2011

DOI: https://doi.org/10.1515/sg-2011-0037

Keywords
Microsatellite, endangered species, conservation

© 2011 G. Marconi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primer Note: Microsatellite-AFLP development for Araucaria araucana (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests

Published Online: Aug 05, 2017

Page range: 285 - 288

Received: Jun 14, 2011

DOI: https://doi.org/10.1515/sg-2011-0037

KeywordsMicrosatellite, endangered species, conservation

© 2011 G. Marconi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Microsatellite, endangered species, conservation