<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A proper identification of clones is necessary in clonal forestry and will help to protect the legitimate interests of breeders, growers and industry. Twenty-four of the <italic>Eucalyptus</italic> clones most widely cultivated in China were analyzed using a set of 24 microsatellite markers to develop their DNA-based fingerprints and exploit the genetic variations. A total of 286 alleles were detected, averaging at 11.9 alleles per marker locus. All the microsatellites were polymorphic among the clones investigated. The observed heterozygosity (<italic>Ho</italic>) varied with locus between 0.500 and 1.000 with a mean of 0.885. The 24 clones could be uniquely fingerprinted based on their multilocus genotypes at a minimum of three loci (Embra169, Embra72 and Embra2). The dendrogram constructed from the genotypic similarity coefficients separated the 24 clones into three groups, matching essentially the historically known or speculated clonal origins. Clones T13, Guanglin-5 and Guanglin-9 turned out to be full siblings of cross DH32 while the DH201-2 sampled here appeared to be mislabelled.</p></abstract>

A proper identification of clones is necessary in clonal forestry and will help to protect the legitimate interests of breeders, growers and industry. Twenty-four of the Eucalyptus clones most widely cultivated in China were analyzed using a set of 24 microsatellite markers to develop their DNA-based fingerprints and exploit the genetic variations. A total of 286 alleles were detected, averaging at 11.9 alleles per marker locus. All the microsatellites were polymorphic among the clones investigated. The observed heterozygosity (Ho) varied with locus between 0.500 and 1.000 with a mean of 0.885. The 24 clones could be uniquely fingerprinted based on their multilocus genotypes at a minimum of three loci (Embra169, Embra72 and Embra2). The dendrogram constructed from the genotypic similarity coefficients separated the 24 clones into three groups, matching essentially the historically known or speculated clonal origins. Clones T13, Guanglin-5 and Guanglin-9 turned out to be full siblings of cross DH32 while the DH201-2 sampled here appeared to be mislabelled.

Microsatellite-based Genotyping of the Commercial <italic>Eucalyptus</italic> Clones Cultivated in China

Research Institute of Tropical Forestry and National Key Laboratory of Forest Tree Genetics and Breeding

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Microsatellite-based Genotyping of the Commercial  Clones Cultivated in China

A proper identification of clones is necessary in clonal forestry and will help to protect the legitimate interests of breeders, growers and industry....

Microsatellite-based Genotyping of the Commercial Eucalyptus Clones Cultivated in China

Published Online: Aug 05, 2017

Page range: 216 - 223

Received: Jan 12, 2011

DOI: https://doi.org/10.1515/sg-2011-0029

Keywords
Microsatellite, clone, genotyping, genetic variation

© 2011 F. Li et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Microsatellite-based Genotyping of the Commercial Eucalyptus Clones Cultivated in China

Published Online: Aug 05, 2017

Page range: 216 - 223

Received: Jan 12, 2011

DOI: https://doi.org/10.1515/sg-2011-0029

KeywordsMicrosatellite, clone, genotyping, genetic variation

© 2011 F. Li et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Microsatellite, clone, genotyping, genetic variation