<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This paper describes a diagnostic system to verify interspecific hybrids between <italic>Acacia mangium</italic> and <italic>A. auriculiformis</italic> using single nucleotide polymorphism (SNP) markers. Forty-eight DNA fragments were selected based on random amplified polymorphic DNAs (RAPD) amplified across 48 individuals from each parental species, and were transformed into 44 sequence-characterized amplified region (SCAR) markers. Five SNP markers that generated species-specific alleles for each species were selected from the 28 sequenced SCARs. A multiplex single nucleotide primer extension (SNuPE) analyses of the five SNPs using 40 <italic>A. mangium</italic>, 40 <italic>A. auriculiformis</italic> and 16 <italic>Acacia</italic> hybrids showed high discrimination power. This diagnostic system, with high discriminatory ability, provides a highly reliable and fast method for identifying interspecific hybrids of <italic>A. mangium</italic> and <italic>A. auriculiformis</italic>.</p></abstract>

This paper describes a diagnostic system to verify interspecific hybrids between Acacia mangium and A. auriculiformis using single nucleotide polymorphism (SNP) markers. Forty-eight DNA fragments were selected based on random amplified polymorphic DNAs (RAPD) amplified across 48 individuals from each parental species, and were transformed into 44 sequence-characterized amplified region (SCAR) markers. Five SNP markers that generated species-specific alleles for each species were selected from the 28 sequenced SCARs. A multiplex single nucleotide primer extension (SNuPE) analyses of the five SNPs using 40 A. mangium, 40 A. auriculiformis and 16 Acacia hybrids showed high discrimination power. This diagnostic system, with high discriminatory ability, provides a highly reliable and fast method for identifying interspecific hybrids of A. mangium and A. auriculiformis.

Diagnosis of interspecific hybrids between <italic>Acacia mangium</italic> and <italic>A. auriculiformis</italic> using single nucleotide polymorphism (SNP) markers

Graduate School of Bioresource and Bioenvironmental Science

The Center for Forest Biotechnology and Tree Improvement

Aibi Lake Wetland National Nature Reserve Administration Bureau

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Diagnosis of interspecific hybrids between  and  using single nucleotide polymorphism (SNP) markers

This paper describes a diagnostic system to verify interspecific hybrids between Acacia mangium and A. auriculiformis using single nucleotide polymorphism...

Diagnosis of interspecific hybrids between Acacia mangium and A. auriculiformis using single nucleotide polymorphism (SNP) markers

Published Online: Aug 05, 2017

Page range: 85 - 92

Received: Nov 04, 2009

DOI: https://doi.org/10.1515/sg-2011-0012

Keywords
interspecific hybrid, PCR, RAPD, SCAR, SNP

© 2011 Vivi Yuskianti et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Diagnosis of interspecific hybrids between Acacia mangium and A. auriculiformis using single nucleotide polymorphism (SNP) markers

Published Online: Aug 05, 2017

Page range: 85 - 92

Received: Nov 04, 2009

DOI: https://doi.org/10.1515/sg-2011-0012

Keywordsinterspecific hybrid, PCR, RAPD, SCAR, SNP

© 2011 Vivi Yuskianti et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
interspecific hybrid, PCR, RAPD, SCAR, SNP