<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We developed 32 microsatellite markers from an enriched genomic DNA library of hinoki (<italic>Chamaecyparis obtusa</italic>), one of the most important Japanese forestry conifer species. From a total of 1,056 cloned plasmids, 96 sequence-specific primer pairs were designed from 110 candidate clones. We selected 32 primers that showed successful amplification and marked polymorphism and evaluated their characteristics using DNA from 38 <italic>C. obtusa</italic> elite trees planted in the Forest Tree Breeding Center. The markers were highly polymorphic, with the number of alleles ranging from 8 to 32 (mean: 20.09), and expected heterozygosity ranging from 0.811 to 0.958 (mean: 0.901). Progress in breeding projects and studies of the ecological genetics of this species can be expected through the use of this enlarged marker pool.</p></abstract>

We developed 32 microsatellite markers from an enriched genomic DNA library of hinoki (Chamaecyparis obtusa), one of the most important Japanese forestry conifer species. From a total of 1,056 cloned plasmids, 96 sequence-specific primer pairs were designed from 110 candidate clones. We selected 32 primers that showed successful amplification and marked polymorphism and evaluated their characteristics using DNA from 38 C. obtusa elite trees planted in the Forest Tree Breeding Center. The markers were highly polymorphic, with the number of alleles ranging from 8 to 32 (mean: 20.09), and expected heterozygosity ranging from 0.811 to 0.958 (mean: 0.901). Progress in breeding projects and studies of the ecological genetics of this species can be expected through the use of this enlarged marker pool.

Primer Note: Development of Highly Polymorphic Nuclear Microsatellite Markers for Hinoki (<italic>Chamaecyparis obtusa</italic>)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primer Note: Development of Highly Polymorphic Nuclear Microsatellite Markers for Hinoki ()

We developed 32 microsatellite markers from an enriched genomic DNA library of hinoki (Chamaecyparis obtusa), one of the most important Japanese forestry...

Primer Note: Development of Highly Polymorphic Nuclear Microsatellite Markers for Hinoki (Chamaecyparis obtusa)

Published Online: Aug 05, 2017

Page range: 62 - 65

Received: Nov 04, 2009

DOI: https://doi.org/10.1515/sg-2011-0008

Keywords
microsatellites, forest tree breeding, genetic resources, ecological genetics

© 2011 M. G. Iwaizumi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primer Note: Development of Highly Polymorphic Nuclear Microsatellite Markers for Hinoki (Chamaecyparis obtusa)

Published Online: Aug 05, 2017

Page range: 62 - 65

Received: Nov 04, 2009

DOI: https://doi.org/10.1515/sg-2011-0008

Keywordsmicrosatellites, forest tree breeding, genetic resources, ecological genetics

© 2011 M. G. Iwaizumi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
microsatellites, forest tree breeding, genetic resources, ecological genetics