<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Successful experiments in molecular biology require good knowledge about various methods and protocols. In molecular biology, nucleic acid manipulation is the essence, starting with the quality of extraction and ending with several analysis assays (PCR, RT-PCR, qPCR, PCR arrays, molecular cloning, etc). Though many of these are so called “standardized”, in practice there are many variables that can influence the outcome of the experiment. Due to the importance of optimal primer design in PCR assays, we will focus on primer designing and checking software, but we also present other useful free tools that can help researchers in the molecular biology field</p></abstract>

Successful experiments in molecular biology require good knowledge about various methods and protocols. In molecular biology, nucleic acid manipulation is the essence, starting with the quality of extraction and ending with several analysis assays (PCR, RT-PCR, qPCR, PCR arrays, molecular cloning, etc). Though many of these are so called “standardized”, in practice there are many variables that can influence the outcome of the experiment. Due to the importance of optimal primer design in PCR assays, we will focus on primer designing and checking software, but we also present other useful free tools that can help researchers in the molecular biology field

From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

Department of Microbiology, University of Medicine and Pharmacy Tirgu-Mures, Romania; / Institute of Virology, University of Zurich, Switzerland

Department of genetics, University of Medicine and Pharmacy of Tîrgu Mureș, Romania

Department of Laboratory Medicine, University of Medicine and Pharmacy of Tîrgu Mureș, Romania

Institute of Virology, University of Zurich, Switzerland

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Successful experiments in molecular biology require good knowledge about various methods and protocols. In molecular biology, nucleic acid manipulation is the...

From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

Published Online: Jun 23, 2015

Page range: 155 - 158

Received: May 03, 2015

Accepted: Jun 06, 2015

DOI: https://doi.org/10.1515/rrlm-2015-0022

Keywords
primer design, free software, molecular biology

© by Adrian Man

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

Published Online: Jun 23, 2015

Page range: 155 - 158

Received: May 03, 2015

Accepted: Jun 06, 2015

DOI: https://doi.org/10.1515/rrlm-2015-0022

Keywordsprimer design, free software, molecular biology

© by Adrian Man

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
primer design, free software, molecular biology