<abstract xmlns="http://www.w3.org/1999/xhtml"> Background/Aim: Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with risk of developing chronic periapical lesion (CPL). Gene polymorphisms are contributing factors in the pathogenesis of the inflammation. The aim of this study was to analyze polymorphism in the gene MMP-8 and its association and influence on clinical manifestation of CPL. Methods: A total of 240 unrelated Macedonian subjects were included in the present study. Polymorphism -799 C/T in the gene MMP-8 detected with restriction enzyme BgIII was studied in 120 patients with CPL and 120 controls without any signs of chronic or acute inflammatory process in the jaw. The amplification of the region of selected gene was made with polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: Our results showed that there were differences in the allele and genotype frequencies of the MMP8 -799 C/T polymorphisms between patients with CPL and controls (p &lt; 0.05). Also this study suggests that MMP-8 polymorphism -799 C/T was a risk for expression of CPL (OR=8.27&lt;3.44&lt;OR&lt;20.26). Conclusion: The detection of this genetic polymorphism is relevant for obtaining providential treatment of patients who are at high risk of chronic periapical inflammation.</abstract>

Background/Aim: Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with risk of developing chronic periapical lesion (CPL). Gene polymorphisms are contributing factors in the pathogenesis of the inflammation. The aim of this study was to analyze polymorphism in the gene MMP-8 and its association and influence on clinical manifestation of CPL. Methods: A total of 240 unrelated Macedonian subjects were included in the present study. Polymorphism -799 C/T in the gene MMP-8 detected with restriction enzyme BgIII was studied in 120 patients with CPL and 120 controls without any signs of chronic or acute inflammatory process in the jaw. The amplification of the region of selected gene was made with polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: Our results showed that there were differences in the allele and genotype frequencies of the MMP8 -799 C/T polymorphisms between patients with CPL and controls (p < 0.05). Also this study suggests that MMP-8 polymorphism -799 C/T was a risk for expression of CPL (OR=8.27<3.44<OR<20.26). Conclusion: The detection of this genetic polymorphism is relevant for obtaining providential treatment of patients who are at high risk of chronic periapical inflammation.

Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions

Stomatological clinical centre "St Pantelemon", Department of Oral Surgery, Vodnjanska 17; 1000 Skopje, R. Macedonia

Study for General Stomatology, Faculty of Medical Sciences, University "Goce Delcev" – Stip, R. Macedonia

Department of Restorative Dentistry and Endodontic, Faculty of Dentistry, "Ss. Cyril and Methodius" University, Skopje, R. Macedonia

Department of Pediatric and Preventive Dentistry, Faculty of Dentistry, "Ss. Cyril and Methodius" University, Skopje, R. Macedonia

PRILOZI

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions"}

Background/Aim: Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with risk of developing chronic periapical lesion (CPL). Gene...

Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions

Published Online: Dec 30, 2015

Page range: 217 - 224

DOI: https://doi.org/10.1515/prilozi-2015-0070

Keywords
chronic periapical lesion, single nucleotide polymorphism, matrix metalloproteinase-8

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions

Published Online: Dec 30, 2015

Page range: 217 - 224

DOI: https://doi.org/10.1515/prilozi-2015-0070

Keywordschronic periapical lesion, single nucleotide polymorphism, matrix metalloproteinase-8

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
chronic periapical lesion, single nucleotide polymorphism, matrix metalloproteinase-8