<abstract xmlns="http://www.w3.org/1999/xhtml"> The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO-Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4 hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4 nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4 hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.</abstract>

The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO-Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4 hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4 nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4 hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.

Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cytotoxicity and genotoxicity of GO-FeO hybrid in cultured mammalian cells

The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO-Fe3O4) on the relative viability and DNA integrity. The...

Cytotoxicity and genotoxicity of GO-Fe₃O₄ hybrid in cultured mammalian cells

Published Online: May 11, 2017

Page range: 27 - 33

DOI: https://doi.org/10.1515/pjct-2017-0004

Keywords
GO-Fe3O4 hybrid, relative viability, DNA fragmentation, micronucleus assay, genotoxicity

© by Magdalena Jedrzejczak-Silicka

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells

Published Online: May 11, 2017

Page range: 27 - 33

DOI: https://doi.org/10.1515/pjct-2017-0004

KeywordsGO-Fe3O4 hybrid, relative viability, DNA fragmentation, micronucleus assay, genotoxicity

© by Magdalena Jedrzejczak-Silicka

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cytotoxicity and genotoxicity of GO-Fe₃O₄ hybrid in cultured mammalian cells

Keywords
GO-Fe3O4 hybrid, relative viability, DNA fragmentation, micronucleus assay, genotoxicity