<abstract xmlns="http://www.w3.org/1999/xhtml">In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission method and optical profiler. Moreover, surface wettability and scratch resistance were determined. It was found that as-deposited coatings were nanocrystalline and had TiO2-anatase structure, built from crystallites in size of 24 nm. The surface of the films was homogenous, composed of closely packed grains and hydrophilic. Due to nanocrystalline structure thin films exhibited good scratch resistance. The results were correlated to the biological activity (<italic>in vitro</italic>) of thin films. Morphological changes of mouse fibroblasts (L929 cell line) after contact with the surface of TiO2 films were evaluated with the use of a contrast-phase microscope, while their viability was tested by MTT colorimetric assay. The viability of cell line upon contact with the surface of nanocrystalline TiO2 film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with surface of examined films was high. This confirms non-cytotoxic effect of TiO2 film surface on mouse fibroblasts.</abstract>

In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission method and optical profiler. Moreover, surface wettability and scratch resistance were determined. It was found that as-deposited coatings were nanocrystalline and had TiO2-anatase structure, built from crystallites in size of 24 nm. The surface of the films was homogenous, composed of closely packed grains and hydrophilic. Due to nanocrystalline structure thin films exhibited good scratch resistance. The results were correlated to the biological activity (in vitro) of thin films. Morphological changes of mouse fibroblasts (L929 cell line) after contact with the surface of TiO2 films were evaluated with the use of a contrast-phase microscope, while their viability was tested by MTT colorimetric assay. The viability of cell line upon contact with the surface of nanocrystalline TiO2 film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with surface of examined films was high. This confirms non-cytotoxic effect of TiO2 film surface on mouse fibroblasts.

Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells

Wroclaw Medical University, Department for Experimental Surgery and Biomaterials Research, Poland

Wroclaw University of Technology, Faculty of Microsystem Electronics and Photonics, Wroclaw, Poland

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of nanocrystalline structure and surface properties of TiO thin films on the viability of L929 cells

In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron...

Influence of nanocrystalline structure and surface properties of TiO₂ thin films on the viability of L929 cells

Published Online: Sep 19, 2015

Page range: 33 - 39

DOI: https://doi.org/10.1515/pjct-2015-0047

Keywords
TiO, surface properties, nanocrystalline thin film, cell morphology, viability of L929 cells

© Małgorzata Osękowska et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells

Published Online: Sep 19, 2015

Page range: 33 - 39

DOI: https://doi.org/10.1515/pjct-2015-0047

KeywordsTiO, surface properties, nanocrystalline thin film, cell morphology, viability of L929 cells

© Małgorzata Osękowska et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of nanocrystalline structure and surface properties of TiO₂ thin films on the viability of L929 cells

Keywords
TiO, surface properties, nanocrystalline thin film, cell morphology, viability of L929 cells