<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The most important morphological characteristic of infections caused by <italic>M. avium subsp. hominissuis</italic> (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with <italic>M. avium subsp. hominissuis</italic>. Examinations have been performed on the samples of <italic>Lnn. jejunales, Lnn. ileocolici</italic> and <italic>Lnn. colici</italic> of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome <italic>M. avium subsp. hominissuis</italic>. The microscopic examination of lymph node samples stained by the routine <italic>hematoxyilin-eosin</italic> (HE) method, showed the presence of granulomatous <italic>lymphadenitis</italic>. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of <italic>granulomatous lymphadenitis</italic> in pigs infected with MAH.</p></abstract>

The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.

The Role of Myofibroblasts in Granulomatous Lymphadenitis in Pigs Naturally Infected with <italic>M. Avium Subsp. Hominissuis</italic>

Scientific Veterinary Institute “Novi Sad”

Veterinary Specialized Institute “Kraljevo”

Department of Animal Husbandry, Faculty of Veterinary Medicine

Department of Pathology, Faculty of Veterinary Medicine

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Role of Myofibroblasts in Granulomatous Lymphadenitis in Pigs Naturally Infected with 

The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is...

The Role of Myofibroblasts in Granulomatous Lymphadenitis in Pigs Naturally Infected with M. Avium Subsp. Hominissuis

Published Online: Mar 15, 2018

Page range: 47 - 53

Received: Oct 13, 2016

Accepted: Nov 23, 2017

DOI: https://doi.org/10.1515/macvetrev-2017-0030

Keywords
granuloma, myofibroblast, TGF-β1, TGF-β1RI

© 2018 Vladimir Polaček et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Role of Myofibroblasts in Granulomatous Lymphadenitis in Pigs Naturally Infected with M. Avium Subsp. Hominissuis

Published Online: Mar 15, 2018

Page range: 47 - 53

Received: Oct 13, 2016

Accepted: Nov 23, 2017

DOI: https://doi.org/10.1515/macvetrev-2017-0030

Keywordsgranuloma, myofibroblast, TGF-β1, TGF-β1RI

© 2018 Vladimir Polaček et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
granuloma, myofibroblast, TGF-β1, TGF-β1RI