<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal origin. The aim of this work was to select a <italic>Yersinia</italic> spp. strain for the plate diffusion method based on sensitivity to a fluoroquinolone, namely flumequine. Another aim was to determine the optimal conditions of the method with the selected strain of <italic>Yersinia ruckeri</italic> CCM 8467 and to determine the detection capability (<italic>CCβ</italic>) of this method for residues of selected fluoroquinolones in milk.<bold>Material and Methods:</bold> Optimum method conditions were set: cell concentration in the test agar at the level of 9.105–106 CFU/mL, discs with a diameter of 12.7 mm, Antimicrobial Inhibitor Test Agar with a pH of 6.0, and incubation at 30°C for at least 18 h and up to 24 h.<bold>Results:</bold> With respect to the maximum residue limit (MRL), the <italic>Y. ruckeri</italic> plate method demonstrated the lowest sensitivity to flumequine. The <italic>CCβ</italic> of the method for flumequine was in the concentration of 100 μg/L−1 (twice the MRL). The study also confirmed that the method exhibits very good sensitivity to the other tested fluoroquinolones, which were marbofloxacin (30 μg/L, 0.4 MRL), ciprofloxacin (10 μg/L, 0.1 MRL), and enrofloxacin (20 μg/L, 0.2 MRL), but lower sensitivity to danofloxacin (42 μg/L, 1.4 MRL).<bold>Conclusion:</bold> The method with the CCM 8467 strain of <italic>Y. ruckeri</italic> showed a higher sensitivity to flumequine than the method with the ATCC 11303 strain of <italic>E. coli</italic>.</abstract>

Introduction: Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal origin. The aim of this work was to select a Yersinia spp. strain for the plate diffusion method based on sensitivity to a fluoroquinolone, namely flumequine. Another aim was to determine the optimal conditions of the method with the selected strain of Yersinia ruckeri CCM 8467 and to determine the detection capability (CCβ) of this method for residues of selected fluoroquinolones in milk.Material and Methods: Optimum method conditions were set: cell concentration in the test agar at the level of 9.105–106 CFU/mL, discs with a diameter of 12.7 mm, Antimicrobial Inhibitor Test Agar with a pH of 6.0, and incubation at 30°C for at least 18 h and up to 24 h.Results: With respect to the maximum residue limit (MRL), the Y. ruckeri plate method demonstrated the lowest sensitivity to flumequine. The CCβ of the method for flumequine was in the concentration of 100 μg/L−1 (twice the MRL). The study also confirmed that the method exhibits very good sensitivity to the other tested fluoroquinolones, which were marbofloxacin (30 μg/L, 0.4 MRL), ciprofloxacin (10 μg/L, 0.1 MRL), and enrofloxacin (20 μg/L, 0.2 MRL), but lower sensitivity to danofloxacin (42 μg/L, 1.4 MRL).Conclusion: The method with the CCM 8467 strain of Y. ruckeri showed a higher sensitivity to flumequine than the method with the ATCC 11303 strain of E. coli.

Detection of fluoroquinolone residues in milk using <italic>Yersinia</italic> spp. strains: towards better sensitivity for flumequine determination

Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology

University of Veterinary and Pharmaceutical Sciences Brno

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of fluoroquinolone residues in milk using  spp. strains: towards better sensitivity for flumequine determination

Introduction: Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal...

Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Published Online: Dec 27, 2017

Page range: 467 - 472

Received: Jun 12, 2017

Accepted: Dec 04, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0061

Keywords
milk, quinolones, residues, plate diffusion method,

© 2017 Pavlína Navrátilová et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Published Online: Dec 27, 2017

Page range: 467 - 472

Received: Jun 12, 2017

Accepted: Dec 04, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0061

Keywordsmilk, quinolones, residues, plate diffusion method,

© 2017 Pavlína Navrátilová et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
milk, quinolones, residues, plate diffusion method,