<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The genomes of nine H5 subtypes of low pathogenic avian influenza virus (LPAIV) strains identified in wild birds in Poland between 2010 and 2015 were sequenced, and their phylogenetic relationship was determined.<bold>Material and Methods:</bold> AIV genome segments were amplified by RT-PCR and the PCR products were sequenced using Sanger method. Phylogenetic trees were generated in MEGA6 software and digital genotyping approach was used to visualise the relationship between analysed strains and other AIVs.<bold>Results:</bold> High genetic diversity was found in the analysed strains as multiple subgroups were identified in phylogenetic trees. In the HA tree, Polish strains clustered in two distinct subclades. High diversity was found for PB2, PB1, PA and NP, since 5-8 sublineages could be distinguished. Each strain had a different gene constellation, although relationship of as much as six out of eight gene segments was observed between two isolates. A relationship with poultry isolates was found for at least one segment of each Polish strain.<bold>Conclusion:</bold> The genome configuration of tested strains indicates extensive reassortment, although the preference for specific gene constellation could be noticed. A significant relationship with isolates of poultry origin underlines the need for constant monitoring of the AIV gene pool circulating in the natural reservoir.</abstract>

Introduction: The genomes of nine H5 subtypes of low pathogenic avian influenza virus (LPAIV) strains identified in wild birds in Poland between 2010 and 2015 were sequenced, and their phylogenetic relationship was determined.Material and Methods: AIV genome segments were amplified by RT-PCR and the PCR products were sequenced using Sanger method. Phylogenetic trees were generated in MEGA6 software and digital genotyping approach was used to visualise the relationship between analysed strains and other AIVs.Results: High genetic diversity was found in the analysed strains as multiple subgroups were identified in phylogenetic trees. In the HA tree, Polish strains clustered in two distinct subclades. High diversity was found for PB2, PB1, PA and NP, since 5-8 sublineages could be distinguished. Each strain had a different gene constellation, although relationship of as much as six out of eight gene segments was observed between two isolates. A relationship with poultry isolates was found for at least one segment of each Polish strain.Conclusion: The genome configuration of tested strains indicates extensive reassortment, although the preference for specific gene constellation could be noticed. A significant relationship with isolates of poultry origin underlines the need for constant monitoring of the AIV gene pool circulating in the natural reservoir.

Phylogenetic study of H5 low pathogenic avian influenza viruses detected in wild birds in Poland in 2010−2015

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: The genomes of nine H5 subtypes of low pathogenic avian influenza virus (LPAIV) strains identified in wild birds in Poland between 2010 and 2015...

Phylogenetic study of H5 low pathogenic avian influenza viruses detected in wild birds in Poland in 2010−2015

Published Online: Dec 27, 2017

Page range: 381 - 389

Received: Jun 23, 2017

Accepted: Nov 02, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0054

Keywords
birds, avian influenza virus, H5 subtype, phylogenetic analysis, Poland

© 2017 Edyta Świętoń et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phylogenetic study of H5 low pathogenic avian influenza viruses detected in wild birds in Poland in 2010−2015

Published Online: Dec 27, 2017

Page range: 381 - 389

Received: Jun 23, 2017

Accepted: Nov 02, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0054

Keywordsbirds, avian influenza virus, H5 subtype, phylogenetic analysis, Poland

© 2017 Edyta Świętoń et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
birds, avian influenza virus, H5 subtype, phylogenetic analysis, Poland