<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The transition period is the most challenging time for dairy cattle, which is characterised not only by negative energy balance but also by fatty tissue mobilisation.<bold>Material and Methods:</bold> The efficiency of energy pathways, β-oxidation in WBC and glycolysis in RBC (based on deoxyglucose transmembrane transport) were estimated. Insulin in blood plasma was determined using ELISA.<bold>Results:</bold> After calving and up to one month after delivery, a significant drop in blood plasma level was noticed, simultaneously with a rise in β-oxidation from 18.93 ±3.64 to 30.32 ±5.28 pmol/min/mg protein in WBC. A strong negative correlation between these two indices (r = −0.68) was found. During the period of transition to lactation an increase in glucose cross-membrane transportation from 41.44 ±4.92 to 50.49 ±6.41 μmol/h/g Hb was observed. A strong positive correlation between glucose transportation in RBC and β-oxidation in WBC (r = 0.71) was noticed. These data are in agreement with results of studies on dairy cows using liver slices from dairy cows in late pregnancy and different stages of lactation, in which changes in gene expression were analysed.<bold>Conclusion:</bold> It seems that measuring fatty acids oxidation and glycolysis using isolated blood cells may be an adequate and relatively simple method for energy state analysis to estimate the state of dairy cow metabolism and animal health.</abstract>

Introduction: The transition period is the most challenging time for dairy cattle, which is characterised not only by negative energy balance but also by fatty tissue mobilisation.Material and Methods: The efficiency of energy pathways, β-oxidation in WBC and glycolysis in RBC (based on deoxyglucose transmembrane transport) were estimated. Insulin in blood plasma was determined using ELISA.Results: After calving and up to one month after delivery, a significant drop in blood plasma level was noticed, simultaneously with a rise in β-oxidation from 18.93 ±3.64 to 30.32 ±5.28 pmol/min/mg protein in WBC. A strong negative correlation between these two indices (r = −0.68) was found. During the period of transition to lactation an increase in glucose cross-membrane transportation from 41.44 ±4.92 to 50.49 ±6.41 μmol/h/g Hb was observed. A strong positive correlation between glucose transportation in RBC and β-oxidation in WBC (r = 0.71) was noticed. These data are in agreement with results of studies on dairy cows using liver slices from dairy cows in late pregnancy and different stages of lactation, in which changes in gene expression were analysed.Conclusion: It seems that measuring fatty acids oxidation and glycolysis using isolated blood cells may be an adequate and relatively simple method for energy state analysis to estimate the state of dairy cow metabolism and animal health.

Effect of pregnancy and stage of lactation on energy processes in isolated blood cells of dairy cows

Department of Reproduction and Clinic of Farm Animals

Wroclaw University of Environmental and Life Sciences

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: The transition period is the most challenging time for dairy cattle, which is characterised not only by negative energy balance but also by fatty...

Effect of pregnancy and stage of lactation on energy processes in isolated blood cells of dairy cows

Published Online: Jun 12, 2017

Page range: 211 - 215

Received: Apr 14, 2016

Accepted: May 10, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0027

Keywords
dairy cows, energetic metabolism, perinatal period, lactation

© 2017 Bogdan Dębski et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of pregnancy and stage of lactation on energy processes in isolated blood cells of dairy cows

Published Online: Jun 12, 2017

Page range: 211 - 215

Received: Apr 14, 2016

Accepted: May 10, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0027

Keywordsdairy cows, energetic metabolism, perinatal period, lactation

© 2017 Bogdan Dębski et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dairy cows, energetic metabolism, perinatal period, lactation