<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> A model of fatty liver in postpartum sheep was established to measure blood paraoxonase 1 (PON1) and other biochemical indicators, which were used to predict fatty liver in sheep.<bold>Material and Methods:</bold> Sheep were assigned into two experimental groups: a fatty liver group (T, n = 10) and a healthy control group (C, n = 5). PON1 enzyme activity towards paraoxon as a substrate was quantified spectrophotometrically. The results were analysed by <italic>t</italic>-test and pearson correlation coefficient. Disease was predicted by binary logistic analysis, and diagnostic thresholds were determined by receiver operatingcharacteristic (ROC) analysis.<bold>Results:</bold> The activity of serum PON1 in group T was significantly decreased (P &lt; 0.05) when compared with C group, and liver lipid content and the levels of serum BHBA, NEFA, and TG were significantly increased (P &lt; 0.05). Thresholds were lower than 74.0 U/mL for PON1, higher than 0.97 mmol/L for β-hydroxybutyrate, higher than 1.29 mmol/L for non-esterified fatty acids, higher than 0.24 mmol/L for triglycerides, and lower than 71.35 g/L for total protein.<bold>Conclusion:</bold> This study verified that PON1, BHBA, NEFA, TG, and TP could be used to predict the risk of fatty liver in sheep.</abstract>

Introduction: A model of fatty liver in postpartum sheep was established to measure blood paraoxonase 1 (PON1) and other biochemical indicators, which were used to predict fatty liver in sheep.Material and Methods: Sheep were assigned into two experimental groups: a fatty liver group (T, n = 10) and a healthy control group (C, n = 5). PON1 enzyme activity towards paraoxon as a substrate was quantified spectrophotometrically. The results were analysed by t-test and pearson correlation coefficient. Disease was predicted by binary logistic analysis, and diagnostic thresholds were determined by receiver operatingcharacteristic (ROC) analysis.Results: The activity of serum PON1 in group T was significantly decreased (P < 0.05) when compared with C group, and liver lipid content and the levels of serum BHBA, NEFA, and TG were significantly increased (P < 0.05). Thresholds were lower than 74.0 U/mL for PON1, higher than 0.97 mmol/L for β-hydroxybutyrate, higher than 1.29 mmol/L for non-esterified fatty acids, higher than 0.24 mmol/L for triglycerides, and lower than 71.35 g/L for total protein.Conclusion: This study verified that PON1, BHBA, NEFA, TG, and TP could be used to predict the risk of fatty liver in sheep.

Serum paraoxonase as an indicator for fatty liver in sheep

Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Serum paraoxonase as an indicator for fatty liver in sheep"}

Introduction: A model of fatty liver in postpartum sheep was established to measure blood paraoxonase 1 (PON1) and other biochemical indicators, which were...

Serum paraoxonase as an indicator for fatty liver in sheep

Published Online: Apr 04, 2017

Page range: 97 - 102

Received: Aug 13, 2016

Accepted: Feb 24, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0012

Keywords
sheep, fatty liver, paraoxonase 1, biochemical indices

© 2017 Yu Cao et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Serum paraoxonase as an indicator for fatty liver in sheep

Published Online: Apr 04, 2017

Page range: 97 - 102

Received: Aug 13, 2016

Accepted: Feb 24, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0012

Keywordssheep, fatty liver, paraoxonase 1, biochemical indices

© 2017 Yu Cao et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
sheep, fatty liver, paraoxonase 1, biochemical indices