<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The aim of the study was to determine microbiota in the cloacal samples of European herring gulls (<italic>Larus argentatus</italic>) and to compare a variety of genes encoding antimicrobial resistance in cultivable and non-cultivable bacteria.<bold>Material and Methods:</bold> Cloacal samples from European herring gulls were collected from a Kaunas city dump. Cultivable microbiota were isolated, their microbial susceptibility was tested, and genes encoding antimicrobial resistance were detected. Additionally, a metagenomic study was performed using Next-Generation Sequencing (NGS).<bold>Results:</bold> In total, 697 different operational taxonomic units at genus level were detected; however, only 63 taxonomic units were detected at the amount of ≥0.1% of the total number of DNA copies. <italic>Catellicoccus marimammalium</italic> was found to have the highest prevalence. The bacterial amount of other genera was up to 5% with the most highly prevalent being <italic>Psychrobacter</italic> (4.7%), <italic>Helicobacter</italic> (4.5%), unclassified Enterococcaceae (3.2%), <italic>Pseudomonas</italic> (2.9%), and <italic>Brachyspira</italic> (2.6%).<bold>Conclusions:</bold><italic>C. marimammalium</italic> are predominant microbiota in the cloacal samples of <italic>Larus argentatus</italic>. This species of gulls is a reservoir of bacteria carrying a wide-spectrum of genes encoding antimicrobial resistance. The same genes were detected in both cultivable microbiota and in the total DNA of the samples.</abstract>

Introduction: The aim of the study was to determine microbiota in the cloacal samples of European herring gulls (Larus argentatus) and to compare a variety of genes encoding antimicrobial resistance in cultivable and non-cultivable bacteria.Material and Methods: Cloacal samples from European herring gulls were collected from a Kaunas city dump. Cultivable microbiota were isolated, their microbial susceptibility was tested, and genes encoding antimicrobial resistance were detected. Additionally, a metagenomic study was performed using Next-Generation Sequencing (NGS).Results: In total, 697 different operational taxonomic units at genus level were detected; however, only 63 taxonomic units were detected at the amount of ≥0.1% of the total number of DNA copies. Catellicoccus marimammalium was found to have the highest prevalence. The bacterial amount of other genera was up to 5% with the most highly prevalent being Psychrobacter (4.7%), Helicobacter (4.5%), unclassified Enterococcaceae (3.2%), Pseudomonas (2.9%), and Brachyspira (2.6%).Conclusions:C. marimammalium are predominant microbiota in the cloacal samples of Larus argentatus. This species of gulls is a reservoir of bacteria carrying a wide-spectrum of genes encoding antimicrobial resistance. The same genes were detected in both cultivable microbiota and in the total DNA of the samples.

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (<italic>Larus argentatus</italic>)

Lithuanian University of Health Sciences, LT-44307 Kaunas,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls ()

Introduction: The aim of the study was to determine microbiota in the cloacal samples of European herring gulls (Larus argentatus) and to compare a variety of...

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Published Online: Apr 04, 2017

Page range: 27 - 35

Received: Aug 27, 2016

Accepted: Mar 06, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0004

Keywords
European herring gulls, microbiome, antimicrobial resistance, genes

© 2017 Lina Merkeviciene et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Published Online: Apr 04, 2017

Page range: 27 - 35

Received: Aug 27, 2016

Accepted: Mar 06, 2017

DOI: https://doi.org/10.1515/jvetres-2017-0004

KeywordsEuropean herring gulls, microbiome, antimicrobial resistance, genes

© 2017 Lina Merkeviciene et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
European herring gulls, microbiome, antimicrobial resistance, genes