Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

1. Alarcón B., García-Cañas V., Cifuentes A., González R., Aznar R.: Simultaneous and sensitive detection of three foodborne pathogens by multiplex PCR, capillary gel electrophoresis, and laser-induced fluorescence. J Agric Food Chem 2004, 52, 7180–7186.10.1021/jf049038bSearch in Google Scholar

2. Beutin L., Miko A., Krause G., Pries K., Haby S., Steege K., Albrecht N.: Identification of human-pathogenic strains of Shiga toxin producing Escherichia coli from food by a combination of serotyping and molecular typing of Shiga toxin genes. Appl Environ Microbiol 2007, 73, 4769–4775.10.1128/AEM.00873-07Search in Google Scholar

3. Bhagwat A.A.: Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR. Int J Food Microbiol 2003, 84, 217–224.10.1016/S0168-1605(02)00481-6Search in Google Scholar

4. EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control): The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2014. EFSA J 2015, 13, 4329, 191 pp.doi:10.2903/j.efsa.2015.432910.2903/j.efsa.2015.4329Search in Google Scholar

5. Garrido A., Chapela M.J., Román B., Fajardo P., Vieites J.M., Gabado A.G.: Development of a multiplex real-time PCR method for simultaneous detection of Salmonella enterica, Shigella flexneri and Listeria monocytogenes in processed food samples. Eur Food Res Technol 2012, 234, 571–580.10.1007/s00217-012-1665-3Search in Google Scholar

6. Garrido A., Chapela M.J., Román B., Fajardo P., Vieites J.M.: A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples. Food Control 2013, 30, 76–85.10.1016/j.foodcont.2012.06.029Search in Google Scholar

7. Garrido A., Chapela M.J., Román B., Fajardo P., Vieites J.M., Gabado A.G.: In-house validation of multiplex real-time PCR method for simultaneous detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes. Int J Food Microbiol 2013, 164, 92–98.10.1016/j.ijfoodmicro.2013.03.02423624537Search in Google Scholar

8. Gehring A.G., Albin D.M., Bhunia A.K., Kim H., Reed S.A., Tu S.-I.: Mixed culture enrichment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, and Yersinia enterocolitica. Food Control 2012, 26, 269–273.10.1016/j.foodcont.2012.01.047Search in Google Scholar

9. Hohmann E.L.: Nontyphoidal salmonellosis. Clin Infect Dis 2001, 32, 263–269.10.1086/31845711170916Search in Google Scholar

10. ISO: International Organization for Standardization, EN ISO 11290-1:1996 – Microbiology of food and animal feeding stuffs – Horizontal method for the detection and enumeration of Listeria monocytogenes – Part 1: Detection method. Geneva, Switzerland.Search in Google Scholar

11. ISO: International Organization for Standardization, EN ISO 16654:2001 – Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Escherichia coli O157. Geneva, Switzerland.Search in Google Scholar

12. ISO: International Organization for Standardization, EN ISO 6579:2002 – Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Salmonella spp. Geneva, Switzerland.Search in Google Scholar

13. ISO: International Organization for Standardization, EN ISO 11290-1/A1:2005 – Microbiology of food and animal feeding stuffs – Horizontal method for the detection and enumeration of Listeria monocytogenes – Part 1: Detection method – Amendment 1: modification of the isolation media, of the haemolysis test and inclusion of precision date. Geneva, Switzerland.Search in Google Scholar

14. ISO: International Organization for Standardization, EN ISO 16140:2003 – Microbiology of food and animal feeding stuffs – Protocol for the validation of alternative methods. Geneva, Switzerland.Search in Google Scholar

15. Kagkli D.M., Weber T.P., Van den Bulcke M., Folloni S., Tozzoli R., Morabito S., Ermolli M., Gribaldo L., Van den Eede G.: Application of the modular approach to an in-house validation study of real-time PCR methods for the detection and serogroup determination of Verocytotoxigenic Escherichia coli. Appl Environ Microbiol 2011, 77, 6954–6963.10.1128/AEM.05357-11318710121856838Search in Google Scholar

16. Kawasaki S., Fratamico P.M., Horikoshi N., Okada Y., Takeshita K., Sameshima T., Kawamoto S.: Multiplex real-time polymerase chain reaction assay for simultaneous detection and quantification of Salmonella species, Listeria monocytogenes, and Escherichia coli O157:H7 in ground pork samples. Foodborne Pathog Dis 2010, 7, 549–554.10.1089/fpd.2009.046520132032Search in Google Scholar

17. Kędrak-Jabłońska A., Budniak S., Szczawińska A., Reksa M., Krupa M., Szulowski K.: Application of SYBR Green I and TaqMan probe-based real-time PCRs for identification of Listeria spp. and Listeria monocytogenes. Bull Vet Inst Pulawy 2015, 59, 489–494.10.1515/bvip-2015-0073Search in Google Scholar

18. Law J.W., Nurul-Syakima A.M., Chan K.G., Lee L.H.: Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. Front Microbiol 2015, 5, 770.10.3389/fmicb.2014.00770429063125628612Search in Google Scholar

19. Lindner J.D.D., Santarelli M., Yamaguishi C.T., Soccol C.R., Neviani E.: Recovery and identification of bovine colostrum microflora using traditional and molecular approaches. Food Technol Biotechnol 2011, 49, 364–368.Search in Google Scholar

20. Lund M., Madsen M.: Strategies for the inclusion of an internal amplification control in conventional and real time PCR detection of Campylobacter spp. in chicken fecal samples. Mol Cell Probes 2006, 20, 92–99.10.1016/j.mcp.2005.10.00216330185Search in Google Scholar

21. Navas J., Ortiz S., Lopez P., Jantzen M.M., Lopez V., Martinez-Suarez J.V.: Evaluation of effects of primary and secondary enrichment for the detection of Listeria monocytogenes by real-time PCR in retail ground chicken meat. Foodborne Pathog Dis 2006, 3, 347–354.10.1089/fpd.2006.3.34717199516Search in Google Scholar

22. Newell D.G., Koopmans M., Verhoef L., Duizer E., Aidara-Kane A., Sprong H., Opsteegh M., Langelaar M., Threfall J., Scheutz F., van der Giessen J., Kruse H.: Food-borne diseases – The challenges of 20 years ago still persist while new ones continue to emerge. Int J Food Microbiol 2010, 139, 3–15.10.1016/j.ijfoodmicro.2010.01.021713249820153070Search in Google Scholar

23. Norrung B., Buncic S.: Microbial safety of meat in the European Union. Meat Sci 2008, 78, 14–24.10.1016/j.meatsci.2007.07.03222062091Search in Google Scholar

24. Omiccioli E., Amagliani G., Brandi G., Magnani M.: A new platform for real-time PCR detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 in milk. Food Microbiol 2009, 26, 615–622.10.1016/j.fm.2009.04.00819527837Search in Google Scholar

25. Padola N.L.: Advances in detection methods for Shiga toxin-producing Escherichia coli (STEC). Front Microbiol 2014, 5, 277.10.3389/fmicb.2014.00277404617724926291Search in Google Scholar

26. Rodriguez A., Rodriguez M., Cordoba J.J., Andrade M.J.: Design of primers and probes for quantitative real-time PCR method. Methods Mol Biol 2015, 1275, 31–56.10.1007/978-1-4939-2365-6_325697650Search in Google Scholar

27. Suo B., He Y., Tu SI., Shi X.: A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis 2010, 7, 619–628.10.1089/fpd.2009.043020113204Search in Google Scholar

28. Suo B., Wang Y.: Evaluation of a multiplex selective enrichment broth SEL for simultaneous detection of injured Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes. Braz J Microbiol 2013, 44, 737–742.10.1590/S1517-83822013000300011Search in Google Scholar

29. Välimaa A.L., Tilsala-Timisjärvi A., Virtanen E.: Rapid detection and identification methods for Listeria monocytogenes in the food chain – A review. Food Control 2015, 55, 103–114.10.1016/j.foodcont.2015.02.037Search in Google Scholar

30. Wittwer C.T., Herrmann M.G., Gundry C.N., Elenitoba-Johnson K.S.J.: Real-time multiplex PCR assays. Methods 2001, 25, 430–442.10.1006/meth.2001.126511846612Search in Google Scholar

31. Wu V.C.: A review of microbial injury and recovery methods in food. Food Microbiol 2008, 25, 735–744.10.1016/j.fm.2008.04.01118620965Search in Google Scholar